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The research on vaccine development for prevention of congenital cytomegalovirus infection

Research Project

Project/Area Number 17K10185
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Embryonic/Neonatal medicine
Research InstitutionGifu Pharmaceutical University (2018-2019)
Fukushima Medical University (2017)

Principal Investigator

Koshizuka Tetsuo  岐阜薬科大学, 薬学部, 准教授 (20416267)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywordsヒトサイトメガロウイルス / ヘルペスウイルス / ユビキチンリガーゼ / Nedd4 / サイトメガロウイルス / ユビキチン / Itch / ユビキチン化 / 先天性サイトメガロウイルス感染症
Outline of Final Research Achievements

Human cytomegalovirus (HCMV) UL42 is a C-terminal anchored membrane protein which interacts with the Nedd4 family Itch at the N-terminal cytoplasmic domain. Although UL42 was thought to be a unique gene of HCMV, we identified the molecules having the same function in other herpesviruses. The expression of UL42 induced the phosphorylation of c-Jun, a transcriptional factor and JNK. On the other hand, c-Jun phosphorylation was not significantly changed in fibroblasts infected with UL42 deficient HCMV, suggesting that the other HCMV genes was important to c-Jun phosphorylation.

Academic Significance and Societal Importance of the Research Achievements

本研究によりHCMV UL42が水痘ワクチン株で大きな変異のある水痘・帯状疱疹ウイルス(VZV)ORF0の相同分子であり、細胞内シグナル伝達を制御する機能を持つことが分かった。UL42もORF0も宿主ユビキチンリガーゼの活性を制御する能力を持ち、細胞内や体内におけるウイルス増殖に関わると考えられる。VZV ORF0が水痘ワクチン株の弱毒化に関わるのであれば、同様の変異を他のヘルペスウイルスに導入することで新たなワクチンの開発に繋がる可能性がある。今後、これらの分子がウイルスの病原性に与える影響を調べる予定である。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2017

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 1 results) Presentation (2 results)

  • [Journal Article] Detection of engraftment of donor-derived antibody producing cells in a lung transplant recipient by anti-cytomegalovirus IgG avidity test2019

    • Author(s)
      Koshizuka Tetsuo、Matsuda Yasushi、Suzuki Hirotoshi、Kanno Ryoko、Ikuta Kazufumi、Kobayashi Takahiro、Kondo Takashi、Okada Yoshinori、Suzutani Tatsuo
    • Journal Title

      Transplant Immunology

      Volume: 53 Pages: 34-37

    • DOI

      10.1016/j.trim.2018.12.003

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Congenital cytomegalovirus infection via a re-infected mother with original antigenic sin: A case report2018

    • Author(s)
      Koshizuka Tetsuo、Toriyabe Kuniaki、Sato Yuko、Ikuta Kazufumi、Ikeda Tomoaki、Suzutani Tatsuo
    • Journal Title

      International Journal of Infectious Diseases

      Volume: 77 Pages: 87-89

    • DOI

      10.1016/j.ijid.2018.10.006

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] A two-step culture method utilizing secreted luciferase recombinant virus for detection of anti-cytomegalovirus compounds2018

    • Author(s)
      Koshizuka Tetsuo、Sato Yuko、Kobiyama Shoe、Oshima Mami、Suzutani Tatsuo
    • Journal Title

      Microbiology and Immunology

      Volume: 62 Issue: 10 Pages: 651-658

    • DOI

      10.1111/1348-0421.12645

    • NAID

      40021702623

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Analysis of relationships between polymorphisms in the genes encoding the pentameric complex and neutralization of clinical cytomegalovirus isolates2018

    • Author(s)
      Kobayashi Ryo、Abe Mao、Oguri Kohdai、Torikai Masaharu、Nishimura Tomohiro、Mori Hiroaki、Koshizuka Tetsuo、Inoue Naoki
    • Journal Title

      Vaccine

      Volume: 36 Issue: 40 Pages: 5983-5989

    • DOI

      10.1016/j.vaccine.2018.08.054

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Herpesviruses possess conserved proteins for interaction with Nedd4 family ubiquitin E3 ligases.2018

    • Author(s)
      Koshizuka T, Kobayashi T, Ishioka K, Suzutani T.
    • Journal Title

      Sci Rep.

      Volume: 8(1) Issue: 1 Pages: 4447-4447

    • DOI

      10.1038/s41598-018-22682-2

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] A method for detection of anti-cytomegalovirus compounds with secreted luciferase recombinant virus.2018

    • Author(s)
      Tetsuo Koshizuka, Yuko Sato, Shoe Kobiyama, Mami Oshima, Tatsuo Suzutani.
    • Organizer
      日本ウイルス学会
    • Related Report
      2018 Research-status Report
  • [Presentation] The ubiquitin ligase regulation mechanism conserved among herpesviruses2017

    • Author(s)
      腰塚哲朗、小林敬広、森康子、錫谷達夫
    • Organizer
      日本ウイルス学会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2022-01-27  

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