Establishment and Clinical Investigations of Graft Immunocomplex Capture Fluorescence Analysis to detect Donor-Specific Antibodies and HLA Antigen Complexes in the Allograft.
Project/Area Number |
17K10518
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Multi-year Fund |
Section | 一般 |
Research Field |
General surgery
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Research Institution | Kyoto Prefectural University of Medicine |
Principal Investigator |
Yoshimura Norio 京都府立医科大学, 医学(系)研究科(研究院), 特任教授 (00191643)
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Co-Investigator(Kenkyū-buntansha) |
中村 緑佐 京都府立医科大学, 医学(系)研究科(研究院), 講師 (30777959)
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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Keywords | 臓器移植 / 拒絶反応 / 抗体関連拒絶反応 / Graft ICFA / 抗ドナー特異的HLA抗体 / DSA / 抗体関連拒絶 / ICFA / Donor specific antibody |
Outline of Final Research Achievements |
Antibody-mediated rejection (AMR) has recently been paid much. The antibodies involved are usually referred to as donor-specific anti- human leukocyte antigen (HLA) antibodies (DSA). The detection of DSA in serum (s-DSA) is generally required as a criterion for the diagnosis of AMR. However, there are sometimes problems with s-DSA. Thus, we applied the novel assessment of intra graft DSA (g-DSA) from biopsy samples that seems to have an advantage over the assessment of s-DSA. The followings are our main findings 1. accurate diagnosis of AMR, 2. early detection of AMR, 3. detection of responsible DSA, 4. an indicator of complete recovery from, 5. g-DSA can be a marker that implys histological and clinical reversibility of AMR. Timely and adequate anti-humoral intervention might reverse the early phase of AMR with complete clearance of g-DSA. graft ICFA routinely will lead to logical decision-making in therapeutic modalities and improved long-term graft survival.
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Academic Significance and Societal Importance of the Research Achievements |
臓器移植後、生検組織を用いimmunocomplex capture fluorescence analysis(ICFA)法を実施することで 1.正診率の向上の寄与、2.早期発見への貢献、3.複数存在する場合に問題となる抗体の同定、4.治療時の改善指標となる、5.治療介入効果予想が可能となり、抗体関連拒絶が大きな臓器移植生着率低下の原因となる臓器移植での移植臓器生着率への向上、患者生存への寄与が期待される意義を持つ。
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Report
(4 results)
Research Products
(13 results)
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[Journal Article] Clearance of Intra-graft Donor Specific Anti-HLA Antibodies in the Early Stage of Antibody-Mediated Rejection following Rituximab and Apheresis Therapy in Renal Transplantation.2019
Author(s)
Nakamura, T., Yoshimura, N., Akioka, K., Shirouzu, T., Kawai, S., Imanishi, Y. et al.
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Journal Title
Transplant Proceedings
Volume: 51
Issue: 5
Pages: 1365-1370
DOI
Related Report
Peer Reviewed
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[Journal Article] Graft Immunocomplex capture fluorescence analysis can detect intra-graft anti MHC antibodies in mice cardiac transplantation.2019
Author(s)
Nakamura, T., Shirouzu, T., Kawai, S., Matsuyama, T., Harada, S., Nobori, S., et al.
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Journal Title
Transplant Proceedings
Volume: 51
Issue: 5
Pages: 1531-1535
DOI
Related Report
Peer Reviewed
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[Journal Article] Graft Immunocomplex capture fluorescence analysis can detect intra-graft anti MHC antibodies in mice cardiac transplantation.2019
Author(s)
Nakamura, T., Shirouzu, T., Kawai, S., Matsuyama, T., Harada, S., Nobori, S., et al.
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Journal Title
Transplant Proceedings
Volume: 51
Issue: 5
Pages: 1531-1535
DOI
Related Report
Peer Reviewed
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[Presentation] Clearance of Intra-graft Donor Specific Anti-HLA Antibodies in the Early Stage of Antibody-Mediated Rejection following Rituximab and Apheresis Therapy in Renal Transplantation.2018
Author(s)
Nakamura, T., Yoshimura, N., Akioka, K., Shirouzu, T., Kawai, S., Imanishi, Y. et al.
Organizer
Transplantation Science Symposium Asian Regional Meeting
Related Report
Int'l Joint Research
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[Presentation] Detection of Intra-Graft Anti-Blood Group A/B Antibodies following Renal Transplantation.2018
Author(s)
Nakamura, T., Yoshimura, N., Shirouzu, T., Kawai, S., Imanishi, Y., Matsuyama, T., et al.
Organizer
Transplantation Science Symposium Asian Regional Meeting
Related Report
Int'l Joint Research
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[Presentation] Graft Immunocomplex capture fluorescence analysis can detect intra-graft anti MHC antibodies in mice cardiac transplantation.2018
Author(s)
Nakamura, T., Yoshimura, N., Shirouzu, T., Kawai, S., Imanishi, Y., Matsuyama, T., et al.
Organizer
The 12th Korea Japan Transplantation Forum
Related Report
Int'l Joint Research
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