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Analysis of brain metastasis specific role of macrophages in breast cancer microenvironment

Research Project

Project/Area Number 17K10546
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field General surgery
Research InstitutionKyoto University

Principal Investigator

SUZUKI EIJI  京都大学, 医学研究科, 准教授 (00612897)

Co-Investigator(Kenkyū-buntansha) 片岡 竜貴  京都大学, 医学研究科, 講師 (20343254)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords乳癌 / 脳転移 / 遺伝子発現解析 / 乳がん / 乳癌脳転移 / トランスレーショナルリサーチ
Outline of Final Research Achievements

Gene expression analysis was performed experimentally with the aim of identifying molecules specific for breast cancer brain metastasis.
A brain metastasis model was established using EMT6, which is a mouse breast cancer cell line. First, EMT6-WT cells were injected into the carotid artery of a mouse, and 4 weeks later, brain tissue and lung tissue were collected, and the tissue was fragmented into cell suspensions and cultured. EMT6 cells were confirmed by culture, and these were identified as brain metastatic cells and designated as EMT6-Br1. EMT6-Br1 cells were injected again from the carotid artery, and brain tissue was cultured again to establish the second generation of EMT6 brain metastatic cells (EMT6-Br2). RNA-seq of EMT6-WT and EMT6-Br2 cells identified scecific genes for brain metastasis. In the future, we will analyze the functions of these genes.

Academic Significance and Societal Importance of the Research Achievements

乳癌脳転移は効果的な治療法が確立されておらず予後不良である。今回乳癌脳転移に関する重要な遺伝子群が同定されることにより新規治療法の開発に繋がることが期待される。ここでは乳癌脳転移細胞に特徴的な遺伝子の同定のみならず乳癌脳転移組織内に存在する免疫細胞など間質細胞の遺伝子的特徴の解析を行う。これにより乳癌細胞間質細胞の相互作用観点からの脳転移制御が可能となり、より治療効果の高い新規治療法の開発ができるものと考える。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Gene expression profile of peripheral blood mononuclear cells may contribute to the identification and immunological classification of breast cancer patients2018

    • Author(s)
      Suzuki Eiji、Sugimoto Masahiro、Kawaguchi Kosuke、Pu Fengling、Uozumi Ryuji、Yamaguchi Ayane、Nishie Mariko、Tsuda Moe、Kotake Takeshi、Morita Satoshi、Toi Masakazu
    • Journal Title

      Breast Cancer

      Volume: 26 Issue: 3 Pages: 282-289

    • DOI

      10.1007/s12282-018-0920-2

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Downregulation of neuropilin-1 on macrophages modulates antibody-mediated tumoricidal activity2017

    • Author(s)
      Kawaguchi Kosuke、Suzuki Eiji、Nishie Mariko、Kii Isao、Kataoka Tatsuki R.、Hirata Masahiro、Inoue Masashi、Pu Fengling、Iwaisako Keiko、Tsuda Moe、Yamaguchi Ayane、Haga Hironori、Hagiwara Masatoshi、Toi Masakazu
    • Journal Title

      Cancer Immunology, Immunotherapy

      Volume: 66 Issue: 9 Pages: 1131-1142

    • DOI

      10.1007/s00262-017-2002-2

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] Exploration of metastatic brain tumor microenvironment specific molecular properties of immune cells and breast cancer cells2019

    • Author(s)
      Eiji Suzuki; Kei Ishii; Takeshi Kotake; Fengling Pu; Eri Hohokabe; Mariko Nishie; Micio Yoshimura; Masakazu Toi
    • Organizer
      Cambridge Healthcare Institute’s 2nd Annual Advancing CNS Biotherapeutics and Crossing the Blood-Brain Barrier
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Gene expression profile of peripheral blood mononuclear cells in breast cancer patients may contribute to the identification and the immunological classification of breast cancer patients by blood test.2017

    • Author(s)
      鈴木栄治
    • Organizer
      米国がん学会
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2021-02-19  

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