Elucidation of functional role of long non-coding RNA in the invasion of hepatocellular carcinoma cells

• Project/Area Number: 17K10662
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Digestive surgery
• Research Institution: National Center for Global Health and Medicine (2018-2019); The University of Tokyo (2017)
• Principal Investigator: Karako (Tang) Takashi (Wei) 国立研究開発法人国立国際医療研究センター, その他部局等, 上級研究員 (00313213)
• Co-Investigator(Kenkyū-buntansha): 秋光 信佳 東京大学, アイソトープ総合センター, 教授 (40294962)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 肝細胞癌 / 長鎖ノンコーディングRNA / 脈管浸潤 / 治療

Outline of Final Research Achievements

The present study aimed to elucidate the role of long-non-coding RNA in the proliferation and the invasion of hepatocellular carcinoma (HCC) cells. We focused the long non-coding RNAs that was suggested to have the relation to the worse prognosis of HCC patients. We transfer the siRNA to HCC cells to reduce the expression of the focused long-non-coding RNA and evaluate the proliferation and the invasion of those HCC cells in vitro. As results, the number of invasive cells was decreased in the PRC1-AS1 knockdown or the LINC00665 knockdown cells compared with the control cells. The proliferation of those cells was not altered. The overexpression of PRC1-AS1 and/or LINC00665 is suggested to have the relation to the HCC prognosis.

Academic Significance and Societal Importance of the Research Achievements

本研究では、肝細胞癌（HCC）細胞におけるPRC1-AS1及びLINC00665の発現上昇が癌細胞の浸潤に関与することを示唆した。この成果は、高頻度な再発が問題となっているHCCの病態悪化における長鎖ノンコーディングRNAの役割の解明に貢献するものと考えられる。また、長鎖ノンコーディングRNAが関与する新しいメカニズムの提示が、開発が立ち遅れているHCCに対する治療法の新規標的の探索に有効であると期待される。

Research Products

• [Journal Article] The impact of apurinic-apyrimidinic endonuclease I on hepatocyte immuno-inflammatory factors and cell apoptosis (2019)
  • Author(s): Sawakami Tatsuo、Sun Zhipeng、Inagaki Yoshinori、Hasegawa Kiyoshi、Tang Wei、Xu Guangzhong、Zhang Nengwei
  • Journal Title: BioScience Trends Volume: 13 Issue: 6 Pages: 539-545
  • DOI: 10.5582/bst.2019.01316
  • NAID: 130007788556
  • ISSN: 1881-7815, 1881-7823
  • Year and Date: 2019-12-31
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Super-aged society: Constructing an integrated information platform of self-recording lifelogs and medical records to support health care in Japan (2019)
  • Author(s): Karako Kenji、Chen Yu、Song Peipei、Tang Wei
  • Journal Title: BioScience Trends Volume: 13 Issue: 3 Pages: 276-278
  • DOI: 10.5582/bst.2019.01124
  • NAID: 130007682090
  • ISSN: 1881-7815, 1881-7823
  • Year and Date: 2019-06-30
  • Peer Reviewed / Open Access
• [Journal Article] On medical application of neural networks trained with various types of data (2018)
  • Author(s): Karako Kenji、Chen Yu、Tang Wei
  • Journal Title: BioScience Trends Volume: 12 Issue: 6 Pages: 553-559
  • DOI: 10.5582/bst.2018.01264
  • NAID: 130007575205
  • ISSN: 1881-7815, 1881-7823
  • Year and Date: 2018-12-31
  • Peer Reviewed / Open Access
• [Journal Article] Cinobufacini inhibits epithelial-mesenchymal transition of human hepatocellular carcinoma cells through c-Met/ERK signaling pathway (2018)
  • Author(s): Qi Fanghua、Wang Jinjing、Zhao Lin、Cai Pingping、Tang Wei、Wang Zhixue
  • Journal Title: BioScience Trends Volume: 12 Issue: 3 Pages: 291-297
  • DOI: 10.5582/bst.2018.01082
  • NAID: 130007405574
  • ISSN: 1881-7815, 1881-7823
  • Year and Date: 2018-06-30
  • Peer Reviewed / Open Access
• [Journal Article] Modeling the rare diseases process in dish (2018)
  • Author(s): Tang Wei
  • Journal Title: Intractable & Rare Diseases Research Volume: 7 Issue: 2 Pages: 72-72
  • DOI: 10.5582/irdr.2018.01048
  • NAID: 130007396129
  • ISSN: 2186-361X, 2186-3644
  • Year and Date: 2018-05-31
  • Peer Reviewed / Open Access
• [Journal Article] Bu?Shen?Ning?Xin decoction suppresses osteoclastogenesis by modulating RANKL/OPG imbalance in the CD4+ T lymphocytes of ovariectomized mice (2018)
  • Author(s): Zhang Jia?Li、Qiu Xue?Min、Zhang Na、Tang Wei、Gober Hans?J???rgen、Li Da?Jin、Wang Ling
  • Journal Title: International Journal of Molecular Medicine Volume: 印刷中
  • DOI: 10.3892/ijmm.2018.3645
  • Peer Reviewed / Open Access
• [Journal Article] Combination of Cinobufacini and Doxorubicin Increases Apoptosis of Hepatocellular Carcinoma Cells through the Fas- and Mitochondria-Mediated Pathways (2017)
  • Author(s): Xia Jufeng、Inagaki Yoshinori、Gao Jianjun、Qi Fanghua、Song Peipei、Han Guohua、Sawakami Tatsuo、Gao Bo、Luo Chuan、Kokudo Norihiro、Hasegawa Kiyoshi、Sakamoto Yoshihiro、Tang Wei
  • Journal Title: Am J Chin Med. Volume: 45 Issue: 07 Pages: 1537-1556
  • DOI: 10.1142/s0192415x17500835
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] The management of hepatocellular carcinoma and advancement of international consensus (2019)
  • Author(s): Tang Wei
  • Organizer: The 10th Asia-Pacific Primary Liver Cancer Expert Meeting
  • Int'l Joint Research
• [Presentation] Clinical research and significance of DCP in patients with HCC. (2018)
  • Author(s): Tang W.
  • Organizer: The Amual Meeting of the Chinese Medical Association of biliary surgery and Chinese Committee of Biliary Surgeons.
• [Presentation] Preoperative Predictors of Tumor Recurrence. (2018)
  • Author(s): Tang W.
  • Organizer: The First Northeast Asia International Hepatobiliary and Pancreatic Surgery Forum.
  • Int'l Joint Research
• [Presentation] Biomarkers -- evaluation of clinical utility in surveillance and early diagnosis for HCC. (2018)
  • Author(s): Tang W.
  • Organizer: 2018 International Hepatobiliary Surgery Forum & The 1th Chinese Transplantation Conference.
  • Int'l Joint Research
• [Presentation] Biomarkers: screening and early diagnosis for hepatocellular carcinoma. (2017)
  • Author(s): Tang W
  • Organizer: National Congress of the diagnosis and treatment of biliary tract tumor 2017.
  • Int'l Joint Research / Invited
• [Presentation] Biomarkers: Evaluation of early diagnosis and prognosis for patients with HCC. (2017)
  • Author(s): Tang W
  • Organizer: 2017 Xinhua International Surgical Forum.
  • Int'l Joint Research / Invited
• [Presentation] DCP expression and the prognosis for Chinese patients with HCC. (2017)
  • Author(s): Tang W
  • Organizer: Jiangsu International Liver Meeting 2017.
  • Int'l Joint Research / Invited