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Analysis of Epithelial mesenchimal trannsion in glioma

Research Project

Project/Area Number 17K10860
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Neurosurgery
Research InstitutionUniversity of Yamanashi

Principal Investigator

KAWATAKI Tomoyuki  山梨大学, 大学院総合研究部, 准教授 (20303406)

Co-Investigator(Kenkyū-buntansha) 齋藤 正夫  山梨大学, 大学院総合研究部, 教授 (90345041)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Keywordsグリオーマ / 上皮間葉転換 / 浸潤能 / ZEB / 膠芽腫 / 浸潤 / ベバシズマブ / 薬剤抵抗性 / 脳神経疾患
Outline of Final Research Achievements

We analyzed the pathogenesis of epithelia mesenchymal transition (EMT) in malignant glioms. We confirmed high expression of EMT transcription factors ZEB1 and ZEB2 with qPCR in 4 kind of human glioblastoma cell lines. In addition, we observed the synergistic inhibition of glioma migration by the siRNA inhibtion of ZEB1/2 expression in vitro. Furthermore, a correlation between expression of ZEB1/2 and histological malignancy was observed in immunohistochemical staining analysis. However, there was no sygnificant synergistic anti- tumor effect of ZEB1/2 inhibition on in the mouse subcutaneous implantation gloma model in vivo. We are going to focus on the relationship between bevacizumab-dependent invasion and these transcription factors.

Academic Significance and Societal Importance of the Research Achievements

本検討では、グリオーマが上皮間葉転換(EMT)という形質転換により、組織における微小環境を巧みに変えて、浸潤や増殖し、これが薬剤抵抗性の根幹であるという仮説に基づき研究を始めた。グリオーマ株やヒトグリオーマ組織におけるEMT関連因子であるZEB1/2の発現が更新し、間葉系の形質を発現し浸潤能を更新している可能性が示唆されたが、Bevacizumab依存性浸潤能には、さらなる検討が必要である。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (4 results)

All 2019 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (3 results)

  • [Journal Article] Expression of ZEBs in gliomas is associated with invasive properties and histopathological grade2018

    • Author(s)
      Suzuki Keiko, Kawataki Tomoyuki, Endo Kaori, Miyazawa Keiji, Kinouchi Hiroyuki, Saitoh Masao.
    • Journal Title

      ONCOLOGY LETTERS

      Volume: 16 Pages: 1758-1764

    • DOI

      10.3892/ol.2018.8852

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] グリオーマにおけるZEB1/2の発現と浸潤能および病理学的悪性度との相関2019

    • Author(s)
      川瀧智之、鈴木景子、齋藤正夫、宮澤恵二、木内博之
    • Organizer
      第19回日本分子脳神経外科学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] グリオーマにおけるZEB1/2の発現と浸潤能および病理学的悪性度との相関2018

    • Author(s)
      川瀧智之、鈴木景子、齋藤正夫、宮澤恵二、木内博之
    • Organizer
      第19回日本分子脳神経外科学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 上皮間葉転換転写因子とグリオーマ浸潤能への関与2017

    • Author(s)
      鈴木景子、川瀧智之、宮澤恵二、齋藤正夫、木内博之
    • Organizer
      第18回日本分子脳神経外科学会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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