Establishment of therapeutic platform using hematopoietic cytokines for cervical spinal cord injury without bony disorders

• Project/Area Number: 17K10903
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neurosurgery
• Research Institution: Yokohama City University
• Principal Investigator: Murata Hidetoshi 横浜市立大学, 医学部, 准教授 (40398524)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2018: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 脊髄症 / 頸髄損傷 / 造血サイトカイン / 顆粒球コロニー刺激因子 / エリスロポイエチン / 治療 / 頚髄損傷 / 脳神経外科学 / 脊髄・脊椎疾患学

Outline of Final Research Achievements

Recently, hematopoietic cytokines (granulocyte colony stimulating factor; G-CSF / erythropoietin; EPO) have been attracting attention for acute spinal cord injury. These cytokines are likely to be effective in clinically safe cervical spinal cord injury consisting of "compressive myelopathy" and "cervical trauma". Using the rat model of chronic spinal cord compression and cervical spinal cord injury without bony disorders, we aimed to clarify the effect of these hematopoietic cytokines to establish the best therapeutic platform.

Academic Significance and Societal Importance of the Research Achievements

圧迫性脊髄症および脊髄損傷に対するG-CSFおよびEPOの効果は明らかとなり、いずれも国際誌 (SPINE2016, PLOS one 2019)で発表した。今後、造血サイトカインの全身性の影響を抑え、より効率的に効果を発揮できれば、臨床的に実用化が可能である。

Research Products

• [Journal Article] 脊髄硬膜動静脈瘻に対する効率的な画像診断の工夫 (2020)
  • Author(s): 宮崎良平 〇村田英俊 佐藤 充 田中貴大 清水信行 末永 潤 中村大志 阿部浩征 長尾景充 立石健祐 山本哲哉
  • Journal Title: 脳卒中の外科 Volume: 48 Pages: 42-48
  • NAID: 130007818430
  • Peer Reviewed
• [Journal Article] 後大脳動脈末梢に発生した感染性動脈瘤に対し、視覚誘発電位(VEP)モニタリング下にコイル塞栓術を行った一例 (2020)
  • Author(s): 新垣勇大 清水信行 村田英俊 五林優子 阿部浩征 長尾景充 佐藤充 末永潤 山本哲哉
  • Journal Title: 脳卒中の外科 Volume: 48 Pages: 134-138
  • Peer Reviewed
• [Journal Article] Development of Quality Indicators of Stroke Centers and Feasibility of Their Measurement Using a Nationwide Insurance Claims Database in Japan ― J-ASPECT Study ― (2019)
  • Author(s): Hasegawa M、Hatayama T、Kondo A、Nagahiro S、Fujimaki T、Amagasaki K、Arita K、Date I、Fujii Y、et al
  • Journal Title: Circulation Journal Volume: 83 Issue: 11 Pages: 2292-2302
  • DOI: 10.1253/circj.CJ-19-0089
  • NAID: 130007733307
  • ISSN: 1346-9843, 1347-4820
  • Year and Date: 2019-10-25
  • Peer Reviewed
• [Journal Article] Human recombinant erythropoietin improves motor function in rats with spinal cord compression-induced cervical myelopathy. (2019)
  • Author(s): Tanaka T, Murata H (equal contribution), Miyazaki R, Yoshizumi T, Sato M, Ohtake M, Tateishi K, Kim P, Yamamoto T.
  • Journal Title: PLoS One. Volume: 10 Issue: 12 Pages: e0214351-e0214351
  • DOI: 10.1371/journal.pone.0214351
  • Peer Reviewed / Open Access
• [Journal Article] 巨大脊髄髄内腫瘍の手術：いかに摘出し、いかに機能を温存するか？ (2019)
  • Author(s): 村田英俊 佐藤 充 宮崎良平 田中貴大 阿部浩征 山本哲哉
  • Journal Title: Spinal Surgery 『脊髄外科』 Volume: 33 Pages: 198-201
  • NAID: 130007705379
  • Peer Reviewed
• [Journal Article] Autologous Transfusion of Blood Aspirated during Suction Decompression in Clipping of Large or Giant Cerebral Aneurysm (2019)
  • Author(s): Ryo Matsuzawa, M.D.*, Hidetoshi Murata, M.D., Ph.D. (equal contribution)*, Mitsuru Sato, M.D., Ryohei Miyazaki, M.D., Takahiro Tanaka, M.D., Nobuyuki Shimizu, M.D., Ph.D., Kensuke Tateishi, M.D., Ph.D., Jun Suenaga, M.D., Ph.D., Tetsuya Yamamoto, M.D., Ph.D.
  • Journal Title: Neurologia medico-chirurgica Volume: 59 Issue: 9 Pages: 351-356
  • DOI: 10.2176/nmc.tn.2018-0299
  • NAID: 130007706969
  • ISSN: 0470-8105, 1349-8029
  • Peer Reviewed / Open Access
• [Journal Article] PI3K/AKT/mTOR pathway alterations promote malignant progression and xenograft formation in oligodendroglial tumors. (2019)
  • Author(s): Tateishi K, Nakamura T, Juratli TA, Williams EA, Matsushita Y, Miyake S, Nishi M, Miller JJ, Ryo A, Yamanaka S, Curry WT, Dias-Santagata D, Yamamoto T, Ichimura K, Batchelor T, Chi AS, Iafrate AJ, Wakimoto H, Cahill DP, et al.
  • Journal Title: Clin Cancer Res. Volume: 印刷中 Issue: 14 Pages: 4375-4387
  • DOI: 10.1158/1078-0432.ccr-18-4144
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 「傍脊柱筋の機能解剖学、姿勢制御と手術アプローチ」 頚椎後方の局所解剖とアプローチ：筋層構築的椎弓形成術 (2019)
  • Author(s): 村田英俊
  • Journal Title: 脊椎脊髄ジャーナル Volume: 32 Pages: 325-332
  • Peer Reviewed
• [Journal Article] 脊髄硬膜動静脈瘻に対する効率的な画像診断の工夫 (2019)
  • Author(s): 宮崎良平 村田英俊 佐藤 充 田中貴大 清水信行 末永 潤 中村大志 阿部浩征 長尾景充 立石健祐 山本哲哉
  • Journal Title: 脳卒中の外科 Volume: 48
  • NAID: 130007818430
  • Peer Reviewed
• [Journal Article] Delayed C5 palsy after anterior cervical decompression surgery; Preoperative foraminal stenosis and postoperative spinal cord shift increase the risk of palsy. (2018)
  • Author(s): Hajime Takase, Hidetoshi Murata, Mitsuru Sato, Takahiro Tanaka, Ryohei Miyazaki, Tetsuya Yoshizumi, Kensuke Tateishi, Nobutaka Kawahara, Tetsuya Yamamoto
  • Journal Title: World Neurosurgery Volume: 120 Pages: e1107-e1119
  • DOI: 10.1016/j.wneu.2018.08.240
  • Peer Reviewed / Open Access
• [Journal Article] Intraventricular Rituximab in Pediatric CD20-positive Refractory Primary Central Nervous System Lymphoma. (2018)
  • Author(s): Wada-Shimosato Y, Ikeda J, Tsujimoto SI, Sasaki K, Yanagimachi M, Kajiwara R, Shiba N, 〇Murata H, Kawahara N, Yamanaka S, Tanoshima R, Ito S.
  • Journal Title: J Pediatr Hematol Oncol. Volume: 17 Issue: 7 Pages: 571-573
  • DOI: 10.1097/mph.0000000000001291
  • Peer Reviewed
• [Journal Article] Three-dimensional multimodality fusion imaging as an educational and planning tool for deep-seated meningiomas. (2018)
  • Author(s): Sato M, Tateishi K, Murata H, Kin T, Suenaga J, Takase H, Yoneyama T, Nishii T, Tateishi U, Yamamoto T, Saito N, Inoue T, Kawahara N.
  • Journal Title: Br J Neurosurg. Volume: 26 Issue: 5 Pages: 1-7
  • DOI: 10.1080/02688697.2018.1485877
  • Peer Reviewed
• [Journal Article] Bow Hunter's Syndrome by Nondominant Vertebral Artery Compression: A Case Report, Literature Review, and Significance of Downbeat Nystagmus as the Diagnostic Clue (2018)
  • Author(s): Iida Yu、Murata Hidetoshi、Johkura Ken、Higashida Testuhiro、Tanaka Takahiro、Tateishi Kensuke
  • Journal Title: World Neurosurgery Volume: 111 Pages: 367-372
  • DOI: 10.1016/j.wneu.2017.12.167
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] ラット圧迫性脊髄症モデルに対するエリスロポエチンの効果 (2018)
  • Author(s): 田中 貴大、村田 英俊、佐藤 充、善積 哲也、宮崎 良平、立石 健祐、金 彪、山本 哲哉
  • Journal Title: 脊髄外科 Volume: 32
  • Peer Reviewed
• [Presentation] 脳神経外科におけるプリズムルーペの有用性 (2020)
  • Author(s): 村田英俊
  • Organizer: 第７回 手技にこだわる脳神経外科ビデオカンファランス
  • Invited
• [Presentation] Surgery of intramedullary spinal cord tumors: Technological progress and achievement limits (2019)
  • Author(s): H Murata, M Sato, R Miyazaki, N Shimizu, K Fujii, T Nakamura, N Ikegaya, K Tateishi, J Suenaga, T Yamamoto
  • Organizer: The 13th APCSS, Kaohsiung, Taiwan, November 9, 2019
  • Int'l Joint Research
• [Presentation] Radiological Risk Factors of Surgical Insufficient Effect for Cervical Degenerative Disease (2019)
  • Author(s): Mitsuru Sato、Hidetoshi Murata、Takahiro Tanaka、Ryohei Miyazaki、Tetsuya Yamamoto
  • Organizer: The 13th APCSS, Kaohsiung, Taiwan, November 9, 2019
  • Int'l Joint Research
• [Presentation] Surgical Treatment of Degenerative Cervical Spine Disease in Elderly Patients (2019)
  • Author(s): Takahiro Tanaka1 , Hidetoshi Murata1 , Mitsuru Sato1 , Ryohei Miyazaki1 , Tetsuya Yamamoto1
  • Organizer: The 13th APCSS, Kaohsiung, Taiwan, November 9, 2019
  • Int'l Joint Research
• [Presentation] Surgery of intramedullary spinal cord tumors: Technological progress and achievement limits (2019)
  • Author(s): Hidetoshi Murata, M.D., Ph.D M Sato, R Miyazaki, N Shimizu, K Fujii, T Nakamura, N Ikegaya, K Tateishi, J Suenaga, T Yamamoto
  • Organizer: The 10th Asia Spine, Seoul, Korea, September 21, 2019
  • Int'l Joint Research
• [Presentation] Surgical Treatment for Degenerative Cervical Spine Disease In Old aged Patients (2019)
  • Author(s): Takahiro Tanaka1 , Hidetoshi Murata1 , Mitsuru Sato1 , Ryohei Miyazaki1 , Tetsuya Yamamoto1
  • Organizer: The 10th Asia Spine, Seoul, Korea, September20- 21, 2019
  • Int'l Joint Research
• [Presentation] Transdural approach for thoracic disc herniation: A technical note (2019)
  • Author(s): Keita Fujii，Hidetoshi Murata, Mitsuru Sato，Tetsuya Yamamoto
  • Organizer: The 10th Asia Spine, Seoul, Korea, September20- 21, 2019
  • Int'l Joint Research
• [Presentation] Surgical Treatment of Degenerative Cervical Spine Disease in Elderly Patients (2019)
  • Author(s): T. Tanaka H. Murata
  • Organizer: Global Spine Congress 2019, Toronto Canada
  • Int'l Joint Research
• [Presentation] 脊髄・脳幹部腫瘍の手術～いかに摘出し、いかに機能を温存するか～ (2019)
  • Author(s): 村田英俊
  • Organizer: 第2回 Blue-line Conference 2019
  • Invited
• [Presentation] 脊髄・脳幹部腫瘍の摘出のコツ：いかに摘出し、いかに機能を温存するか (2019)
  • Author(s): 村田英俊
  • Organizer: 第63回中部脊髄外科ワークショップ
  • Invited
• [Presentation] 「脊髄・脳幹部腫瘍の剥離」 (2019)
  • Author(s): 村田英俊
  • Organizer: 第75回 関東脳神経外科懇話会
  • Invited
• [Presentation] Human Recombinant Erythropoietin Improves Motor function in Chronic Cervical Compression Myelopathy Rat Model (2018)
  • Author(s): Takahiro Tanaka, Hidetoshi Murata, Ryohei Miyazaki, Tetsuya Yoshizumi, Mitsuru Sato, Makoto Ohtake, Kensuke Tateishi, Phyo Kim, Tetsuya Yamamoto
  • Organizer: GLOBAL SPINE CONGRESS 2018
  • Int'l Joint Research
• [Presentation] Human Recombinant Erythropoietin Improves Motor function in Chronic Cervical Compression Myelopathy Rat Model (2018)
  • Author(s): Takahiro Tanaka, Hidetoshi Murata, Ryohei Miyazaki, Tetsuya Yoshizumi, Mitsuru Sato, Makoto Ohtake, Kensuke Tateishi, Phyo Kim, Tetsuya Yamamoto
  • Organizer: The 9th Annual Meeting of ASIA SPINE
  • Int'l Joint Research
• [Presentation] ラット頚椎症性脊髄症モデルに対するエリスロポエチンの効果 (2018)
  • Author(s): 田中 貴大、村田 英俊、佐藤 充、善積 哲也、宮崎 良平、立石 健祐、金 彪、山本 哲哉
  • Organizer: 第33回 日本脊髄外科学会
• [Presentation] Human Recombinant Erythropoietin Improves Motor function in Chronic Cervical Compression Myelopathy Rat Model (2018)
  • Author(s): Takahiro Tanaka, M.D., Hidetoshi Murata, M.D., Ph.D., Ryohei Miyazaki, M.D., Makoto Ohtake, M.D., Ph.D, Mitsuru Sato, M.D., Tetsuya Yoshizumi, M.D., Ph.D., Kensuke Tateishi, M.D., Ph.D., Phyo Kim, M.D., Ph.D., Tetsuya Yamamoto, M.D., Ph.D.
  • Organizer: GLOBAL SPINE CONGRESS 2018
  • Int'l Joint Research
• [Presentation] ラット頚椎症性脊髄症モデルに対するエリスロポエチンの効果：第1報 (2017)
  • Author(s): 田中 貴大、村田 英俊、佐藤 充、善積 哲也、宮崎 良平、金 彪
  • Organizer: 第32回日本脊髄外科学会
• [Presentation] ラット頚椎症性脊髄症モデルに対するエリスロポエチンの効果 (2017)
  • Author(s): 田中 貴大、村田 英俊、佐藤 充、善積 哲也、宮崎 良平、金 彪
  • Organizer: 第76回日本脳神経外科学会総会