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Acquisition of novel naive pluripotent stem cells by activation and regulation of post-transcriptional modification on beta catenin

Research Project

Project/Area Number 17K11037
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Orthopaedic surgery
Research InstitutionKindai University

Principal Investigator

TAKEHARA Toshiyuki  近畿大学, 大学病院, 助教 (60580561)

Co-Investigator(Kenkyū-buntansha) 寺村 岳士  近畿大学, 大学病院, 講師 (40460901)
福田 寛二  近畿大学, 医学部, 教授 (50201744)
末盛 博文  京都大学, ウイルス・再生医科学研究所, 准教授 (90261198)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Keywordsβcatenin / naive型とprimed型 / 多能性幹細胞 / iPS細胞 / 基底状態 / ES細胞 / ヒト多能性幹細胞 / β-catenin / ヒトiPS細胞 / リプログラミング
Outline of Final Research Achievements

The clinical application for regenerative medicine of human iPS cells should be achieved to naive state which shows a more high differentiation potential, self-renwal and genome stability. In this study, we focused on the βcatenin molecule and found that the acquisition of naive human iPS cells was possible without genetic intervention and by altering its post-transcription modification state. These results will refinement the understanding of the pluripotency network and the value of using iPS cells further. In addition, since βcatenin is robustly associated with oncogenic transformation and cell death, the novel function of βcatenin obtained in this study may be an important insight in an unknown cellular phenomenon.

Academic Significance and Societal Importance of the Research Achievements

幅広い領域への再生医療の適用には、高い能力を持つ多能性幹細胞の利用は必須である。しかしながら、現状のヒト多能性幹細胞は増殖能力の低さ、ゲノム不安定性、分化能力の低さを示すことから、これらの問題を解決する必要がある。本研究では、遺伝子組換えを必要とせず、1因子の制御によって非常に能力の高いヒト多能性幹細胞の獲得が可能であった。これはβcateninと多能性ネットワークの新しい関係性を示すと同時に、臨床応用に適したヒト多能性幹細胞を準備することが可能な技術であると考えられる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (3 results)

All 2020 2019

All Presentation (2 results) Patent(Industrial Property Rights) (1 results)

  • [Presentation] 異なる多能性状態:Naive型及びPrimed型に対するβ-cateninの役割2020

    • Author(s)
      竹原俊幸、小野寺勇太、福田寛二、寺村岳士
    • Organizer
      第19回日本再生医療学会総会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 第15回日本生殖発生医学会学術集会2020

    • Author(s)
      竹原俊幸、小野寺勇太、福田寛二、寺村岳士
    • Organizer
      β-cateninを標的とした新たなヒトNaive型多能性幹細胞獲得の試み
    • Related Report
      2019 Annual Research Report
  • [Patent(Industrial Property Rights)] βカテニンこファクター阻害剤によるNaive型多能性幹細胞の作製法2019

    • Inventor(s)
      竹原俊幸
    • Industrial Property Rights Holder
      竹原俊幸
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report

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Published: 2017-04-28   Modified: 2024-12-25  

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