Cell biological significance of the remarkable construction of the human blastocyst called collapse
| Project/Area Number |
17K11215
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Akita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
佐藤 亘 秋田大学, 医学部附属病院, 助教 (10726441)
清水 大 秋田大学, 医学部附属病院, 講師 (60400503)
高橋 和政 秋田大学, 医学部附属病院, 技術系スタッフ (60791910)
佐藤 敏治 秋田大学, 医学系研究科, 助教 (70636183)
三浦 広志 秋田大学, 医学部附属病院, 助教 (80375302)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 胚細胞 / ICM / TE / タイトジャンクション / Na-K ATPase / 胚盤胞 / NA-K ATPase / 発育挙動 / タイムラプス / 発現タンパク質 / 胚選別 / 発現タンパく質 |
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| Outline of Final Research Achievements |
Time-lapse observation and fluorescent immunostaining of mouse blastocysts revealed that the type of hatching and the embryo contraction affected the ectopic distribution of inner cell mass. (Onodera, Kumazawa, Terada PloS ONE 2017). In a study using human blastocysts, the number of cells was actually measured, and it was proved that the number of trophectderm cells in good embryos was significantly higher than that in poor embryos (Iwasawa, Kumazawa, Terada PloS ONE 2019). In the analysis of electrolyte dynamics during embryogenesis using mouse embryos, the temporal and spatial dynamics of Na + and K + from morula to blastocyst were successfully visualized for the first time in the world (Fujishima, Kumazawa, Terada PLoS). ONE 2021). NaK ATPase expression was analyzed and revealed that NaK ATPase consisting of α1 and β3 is important in human blastocysts.
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| Academic Significance and Societal Importance of the Research Achievements |
生殖補助医療技術の向上にはヒト胚についての細胞学的基礎知見が必要である。胚移植に際してより適した胚選択法が求められている。また胚培養環境の検討も十分とは言えない。本研究の成果では、動的挙動が胚自体に与える影響と胚細胞数との関係性から移植胚の選択基準になり得ることを示した。培養環境である培養液では電解質が大きなウェイトを占めている、胚発生における電解質の挙動を知ることは培養環境の向上に大きく資するものである。Na・Kイオンの輸送に関わるタンパク質の構造を明らかにしたことは、胚の機能面の評価につながる。これは生殖補助医療技術の向上につながると期待される。
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Report
(5 results)
Research Products
(15 results)
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[Journal Article] Live visualisation of electrolytes during mouse embryonic development using electrolyte indicators2021
Author(s)
Fujishima A, Takahashi K, Goto M, Hirakawa T, Iwasawa T, Togashi K, Maeda E, Shirasawa H, Miura H, Sato W, Kumazawa Y, Terada Y
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Journal Title
PLoS ONE
Volume: 16
Issue: 1
Pages: e0246337-e0246337
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Cell-free DNA in spent culture medium effectively reflects the chromosomal status of embryos following culturing beyond implantation compared to trophectoderm biopsy.2021
Author(s)
Shitara A, Takahashi K, Goto M, Takahashi H, Iwasawa T, Onadera Y, Makino K, Miura H, Shirasawa H, Sato W, Kumazawa Y, Terada Y
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Journal Title
PLoS ONE
Volume: 16
Issue: 2
Pages: e0246438-e0246438
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The location of “8”-shaped hatching influences inner cell mass formation in mouse blastocysts.2017
Author(s)
Yohei Onodera, Kazumasa Takahashi, Mayumi Goto, Mibuki Anzai, Natsuki Ono, Hiromitsu Shirasawa, Wataru Sato, Hiroshi Miura, Naoki Sato, Akira Sato, Yukiyo Kumazawa, Yukihiro Terada
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Journal Title
PLoS ONE
Volume: 12
Issue: 4
Pages: e0175150-e0175150
DOI
Related Report
Peer Reviewed / Open Access
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