| Project/Area Number |
17K11237
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Shimane University |
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Principal Investigator |
Kanasaki Haruhiko 島根大学, 学術研究院医学・看護学系, 准教授 (10325053)
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| Co-Investigator(Kenkyū-buntansha) |
折出 亜希 島根大学, 学術研究院医学・看護学系, 講師 (00423278)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 月経異常 / 女性内分泌 / 生殖内分泌 / キスペプチン / アクチビン / インヒビン / フォリスタチン / 視床下部 / GnRH / 下垂体 / ゴナドトロピン / 神経内分泌 / ＨＰＧ ｑａｘｉｓ |
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| Outline of Final Research Achievements |
In the mHypoA-55 ARC cells, activin significantly increased Kiss-1 gene expression. Follistatin alone reduced Kiss-1 expression within these cells. Interestingly, activin-induced Kiss-1 gene expression was completely abolished by follistatin. Inhibin A, but not inhibin B reduced Kiss-1 expression. Activin-increased Kiss-1 expression was also abolished by inhibin A. Pretreatment of the cells with follistatin or inhibin A significantly inhibited kisspeptin- or GnRH-induced Kiss-1 gene expression in mHypoA-55 cells. The subunits that compose activin and inhibin, as well as follistatin were expressed in mHypoA-55 cells. We found that expression of the inhibin  subunit and follistatin genes was modulated in the presence of E2 in mHypoA-55 ARC cells.
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| Academic Significance and Societal Importance of the Research Achievements |
女性の月経周期は視床下部-下垂体-性腺軸により精巧に調節されているが、体重の増減や過度な運動、ストレスの存在などにより簡単に破綻する。視床下部-下垂体-性腺軸の頂点に存在するとされる脳内キスペプチンニューロンの制御機構を知ることは、女性内分泌の詳細を解き明かす手掛かりになると思われます。
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