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Placenta-derived mesenchymal stem cell and exosome in placental function

Research Project

Project/Area Number 17K11241
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Obstetrics and gynecology
Research InstitutionNagasaki University

Principal Investigator

MIURA Kiyonori  長崎大学, 医歯薬学総合研究科(医学系), 教授 (00363490)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords胎盤 / 妊娠 / 間葉系幹細胞 / 妊娠高血圧腎症 / microRNA / 母体血漿 / 産科婦人科 / エクソソーム / Preeclampsia / 分子マーカー / 産科婦人科学 / RNA / preeclampsia / 胎盤機能 / mRNA
Outline of Final Research Achievements

We isolated and expanded adequate numbers of cells with characteristic features of mesenchymal stem cells (MSCs) from the chorionic plate (CP-MSCs), chorionic villi (CV-MSCs), and decidua basalis (DB-MSCs) of human term placental tissues. All placenta-derived MSCs expressed pregnancy-associated C14MC microRNA (miRNA) (miR-323-3p). Interestingly, the placenta-specific C19MC miRNAs (miR-518b and miR517a) were clearly expressed in CP-MSCs and CV-MSCs of foetal origin. High-efficiency siRNA transfection was confirmed in twice-passaged CV-MSCs with little toxicity, and microarray analysis was used to screen for miR-518b target genes. Subsequently, we determined the reference values for circulating pregnancy-associated placental miRNAs on C19MC in maternal plasma throughout pregnancy. The reference values for circulating pregnancy-associated placental miRNAs in maternal plasma may contribute to the prediction of a preeclampsia before the onset of disease.

Academic Significance and Societal Importance of the Research Achievements

胎盤由来MSCにおける妊娠関連microRNAと病態形成との詳細な分子機序についてはさらなる研究が必要であるが、胎盤由来MSCを利用することで妊娠関連疾患の分子メカニズムの解明に有用なツールとなりうることが示唆された。そして、母体血漿中の妊娠関連microRNAを定量化することが妊娠高血圧症候群の病態評価につながる可能性が示唆された。本研究の成果は、産科疾患の病態解明ならびに早期診断に貢献すると期待される。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (48 results)

All 2021 2020 2019 2018 2017 Other

All Journal Article (27 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 21 results,  Open Access: 4 results,  Acknowledgement Compliant: 1 results) Presentation (16 results) (of which Int'l Joint Research: 2 results,  Invited: 12 results) Book (1 results) Remarks (4 results)

  • [Journal Article] Cervical Superficially Invasive Squamous Cell Carcinoma With Supraclavicular Lymph Node Metastasis: A Case Report2020

    • Author(s)
      Kawashita Sayaka、Matsuda Katsuya、Matsuwaki Takahiro、Kurohama Hirokazu、Ito Masahiro、Kishikawa Masao、Miura Kiyonori、Nakashima Masahiro
    • Journal Title

      International Journal of Gynecological Pathology

      Volume: 40 Issue: 1 Pages: 78-83

    • DOI

      10.1097/pgp.0000000000000679

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Paediatric-onset haploinsufficiency of A20 associated with a novel and de novo nonsense TNFAIP3 mutation2020

    • Author(s)
      Endo Yushiro、Funakoshi Yasutomo、Koga Tomohiro、Furukawa Kaori、Sasaki Daisuke、Miura Kiyonori、Yanagihara Katsunori、Moriuchi Hiroyuki、Kawakami Atsushi
    • Journal Title

      Rheumatology

      Volume: 59 Issue: 11 Pages: e85-e87

    • DOI

      10.1093/rheumatology/keaa206

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] HTLV-1 targets human placental trophoblasts in seropositive pregnant women2020

    • Author(s)
      Tezuka Kenta、Fuchi Naoki、Okuma Kazu、Tsukiyama Takashi、Miura Shoko、Hasegawa Yuri、Nagata Ai、Komatsu Nahoko、Hasegawa Hiroo、Sasaki Daisuke、Sasaki Eita、Mizukami Takuo、Kuramitsu Madoka、Matsuoka Sahoko、Yanagihara Katsunori、Miura Kiyonori、Hamaguchi Isao
    • Journal Title

      Journal of Clinical Investigation

      Volume: 130 Issue: 11 Pages: 6171-6186

    • DOI

      10.1172/jci135525

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] The Incidence of Dichorionic Diamniotic Twin Pregnancy After Single Blastocyst Embryo Transfer and Zygosity: 8 Years of Single-Center Experience2020

    • Author(s)
      Konno Hiroko、Murakoshi Takeshi、Miura Kiyonori、Masuzaki Hideaki
    • Journal Title

      Twin Research and Human Genetics

      Volume: 23 Issue: 1 Pages: 51-54

    • DOI

      10.1017/thg.2020.5

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Significance of p53-Binding Protein 1 Nuclear Foci in Cervical Squamous Intraepithelial Lesions: Association With High-Risk Human Papillomavirus Infection and P16INK4a Expression2020

    • Author(s)
      Kawashita Sayaka、Matsuda Katsuya、Kondo Hisayoshi、Kitajima Yuriko、Hasegawa Yuri、Shimada Takako、Kitajima Michio、Miura Kiyonori、Nakashima Masahiro、Masuzaki Hideaki
    • Journal Title

      Cancer Control

      Volume: 27 Issue: 1 Pages: 1-8

    • DOI

      10.1177/1073274819901170

    • NAID

      120006988289

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] 妊娠初期の見方と所見2020

    • Author(s)
      三浦清徳
    • Journal Title

      臨床産科婦人科

      Volume: 74 Pages: 14-22

    • Related Report
      2019 Research-status Report
  • [Journal Article] Classification of factors involved in nonreportable results of noninvasive prenatal testing (NIPT) and prediction of success rate of second NIPT2019

    • Author(s)
      Suzumori N, Sekizawa A, Samura O, Yotsumoto J, Sago H,et al
    • Journal Title

      Prenatal Diagnosis

      Volume: 39(2) Issue: 2 Pages: 100-106

    • DOI

      10.1002/pd.5408

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] IFPA meeting 2018 workshop report I: Reproduction and placentation among ocean-living species; placental imaging; epigenetics and extracellular vesicles in pregnancy.2019

    • Author(s)
      Acharya G, Bartolomei M, Carter AM, Chamley L, Cotton CF, Hasegawa J, Hasegawa Y, Hayakawa S, Kawaguchi M, Konwar C, Magawa S, Miura K, Nishi H, Salomon C, Sato K, Soejima H, Soma H, Sorensen A, Takahashi H, Tomita T, Whittington CM, Yuan V, O'Tierney-Ginn P.
    • Journal Title

      Placenta

      Volume: 18 Pages: 31201-31203

    • DOI

      10.1016/j.placenta.2019.02.003

    • Related Report
      2019 Research-status Report 2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Malignant transformation from mature cystic teratoma of the ovary2019

    • Author(s)
      Shimada Takako、Higashijima Ai、Fukushima Ai、Komatsu Nahoko、Noguchi Masashi、Ohashi Kazuaki、Hasegawa Yuri、Miura Kiyonori
    • Journal Title

      Journal of Obstetrics and Gynaecology Research

      Volume: 45 Issue: 9 Pages: 1957-1960

    • DOI

      10.1111/jog.14043

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Huge uterine fibroid arising from primary uterine cervical diverticulum: a case report and review of the literatures2019

    • Author(s)
      Noguchi Masashi、Kitajima Michio、Abe Shuhei、Murakami Naoko、Kitajima Yuriko、Miura Kiyonori、Masuzaki Hideaki
    • Journal Title

      Journal of Obstetrics and Gynaecology

      Volume: 39 Issue: 8 Pages: 1186-1187

    • DOI

      10.1080/01443615.2019.1588237

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] Comprehensive immune complexome analysis detects disease-specific immune complex antigens in seminal plasma and follicular fluids derived from infertile men and women2019

    • Author(s)
      Murakami Naoko、Kitajima Michio、Ohyama Kaname、Aibara Nozomi、Taniguchi Ken、Wei Mian、Kitajima Yuriko、Miura Kiyonori、Masuzaki Hideaki
    • Journal Title

      Clinica Chimica Acta

      Volume: 495 Pages: 545-551

    • DOI

      10.1016/j.cca.2019.05.031

    • NAID

      120006987696

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Impaired early‐phase suppression of glucagon secretion after glucose load is associated with insulin requirement during pregnancy in gestational diabetes2019

    • Author(s)
      Horie Ichiro、Haraguchi Ai、Ito Ayako、Nozaki Aya、Natsuda Shoko、Akazawa Satoru、Mori Yoshitaka、Ando Takao、Higashijima Ai、Hasegawa Yuri、Yoshida Atsushi、Miura Kiyonori、Masuzaki Hideaki、Kawakami Atsushi、Abiru Norio
    • Journal Title

      Journal of Diabetes Investigation

      Volume: 11 Issue: 1 Pages: 232-240

    • DOI

      10.1111/jdi.13096

    • NAID

      120006988318

    • Related Report
      2019 Research-status Report
    • Peer Reviewed
  • [Journal Article] 産後の子宮内容物排出異常に対して駆癌血剤が有効であった7例2019

    • Author(s)
      高野玲、増﨑雅子、吉田敦、三浦清徳、増﨑英明
    • Journal Title

      産婦人科漢方研究のあゆみ

      Volume: 36 Pages: 94-98

    • Related Report
      2019 Research-status Report
  • [Journal Article] 胎盤限局性モザイク2019

    • Author(s)
      三浦清徳
    • Journal Title

      三浦清徳

      Volume: - Pages: 164-168

    • Related Report
      2019 Research-status Report
  • [Journal Article] NIPTで性染色体数的異常は検出できるか?2019

    • Author(s)
      三浦清徳、三浦生子、増崎英明
    • Journal Title

      臨床婦人科産科

      Volume: 73 Pages: 253-257

    • Related Report
      2019 Research-status Report
  • [Journal Article] 胎盤とモザイク2019

    • Author(s)
      三浦清徳
    • Journal Title

      医学のあゆみ

      Volume: 269 Pages: 775-778

    • Related Report
      2019 Research-status Report
  • [Journal Article] 心肺停止し搬送された妊婦に死戦期帝王切開術を施行した一例2019

    • Author(s)
      渡邊灯、長谷川ゆり、永田典子、村上直子、東島愛、松本加奈子、北島道夫、三浦清徳、増﨑英明
    • Journal Title

      日本産婦人科・新生児血液学会誌

      Volume: 269 Pages: 39-40

    • Related Report
      2019 Research-status Report
  • [Journal Article] Development of reference material with assigned value for human T-cell leukemia virus type 1 quantitative PCR in Japan2018

    • Author(s)
      Kuramitsu M、Okuma K、Nakashima M、Sato T、Sasaki D、Hasegawa H、Umeki K、Kubota R、Sasada K、Sobata R、Matsumoto C、Kaneko N、Tezuka K、Matsuoka S、Utsunomiya A、Koh KR、Ogata M、Ishitsuka K、Taki M、Nosaka K、Uchimaru K、Iwanaga M、Sagara Y、Yamano Y、Okayama A、Miura K et al
    • Journal Title

      Microbiology and Immunology

      Volume: 62 Issue: 10 Pages: 673-676

    • DOI

      10.1111/1348-0421.12644

    • NAID

      40021702629

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Maternal age-specific risk for trisomy 21 based on the clinical performance of NIPT and empirically derived NIPT age-specific positive and negative predictive values in Japan2018

    • Author(s)
      Yamada Takahiro、Japan NIPT consortium*、Sekizawa Akihiko、Fujii Yosuke、Hirose Tatsuko、Samura Osamu、Suzumori Nobuhiro、Miura Kiyonori、Sawai Hideaki、Hirahara Fumiki、Murotsuki Jun、Kamei Yoshimasa、Sago Haruhiko
    • Journal Title

      Journal of Human Genetics

      Volume: 63 Issue: 10 Pages: 1035-1040

    • DOI

      10.1038/s10038-018-0453-8

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fetal cell-free DNA fraction in maternal plasma for the prediction of hypertensive disorders of pregnancy2018

    • Author(s)
      Suzumori , Sekizawa A,Yotsumoto J, et al
    • Journal Title

      Eur J Obstet Gynecol Reprod Biol

      Volume: 224 Pages: 165-169

    • DOI

      10.1016/j.ejogrb.2018.03.048

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Reference values for circulating pregnancy-associated microRNAs in maternal plasma and their clinical usefulness in uncomplicated pregnancy and hypertensive disorder of pregnancy2018

    • Author(s)
      Murakami Y, Miura K, Sato S, Higashijima A, Hasegawa Y, Miura S, Yoshiura KI, Masuzaki H
    • Journal Title

      Journal of Obstetrics and Gynaecology Research

      Volume: 印刷中 Issue: 5 Pages: 840-851

    • DOI

      10.1111/jog.13610

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Natural course of HTLV-1 proviral DNA levels in carriers during pregnancy2018

    • Author(s)
      Fuchi N, Miura K, Tsukiyama T, Sasaki D, Ishihara K, Tsuruda K, Hasegawa H, Miura S, Yanagihara K, Masuzaki H
    • Journal Title

      Journal of Infectious Diseases

      Volume: 217 Issue: 9 Pages: 1383-1389

    • DOI

      10.1093/infdis/jiy017

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Decreased cell-free but not exosomal miR-518b in maternal plasma is caused by amniocentesis2018

    • Author(s)
      Miura K, Higashijima A, Hasegawa Y, Miura S, Yoshiura KI, Masuzaki H
    • Journal Title

      Clinical and Experimental Obstetrics & Gynecology

      Volume: 印刷中

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Maternal age-specific risk for trisomy 21 based on the clinical performance of NIPT and empirically derived NIPT age-specific positive and negative predictive values in Japan2018

    • Author(s)
      Yamada T, Sekizawa A, Fujii Y, Hirose T, Samura O, Suzumori N, Miura K, Sawai H, Hirahara F, and Sago H
    • Journal Title

      Journal of Human Genetics

      Volume: 印刷中

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Proviral features of human T cell leukemia virus type 1 in carriers with indeterminate western blot analysis results.2017

    • Author(s)
      Kuramitsu M, Sekizuka T, Yamochi T, Firouzi S, Sato T, Umeki K, Sasaki D, Hasegawa H, Kubota R, Sobata R, Matsumoto C, Kaneko N, Momose H, Araki K, Saito M, Nosaka K, Utsunomiya A, Koh K, Ogata M, Uchimaru K, Iwanaga M, Sagara Y, Yamano Y, et al.
    • Journal Title

      J Clin Microbiol.

      Volume: 55(9) Issue: 9 Pages: 2838-2849

    • DOI

      10.1128/jcm.00659-17

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Current status of non-invasive prenatal testing in Japan.2017

    • Author(s)
      Samura O, Sekizawa A, Suzumori N, Sasaki A, Wada S, Hamanoue H, Hirahara F, Sawai H, Nakamura H, Yamada T, Miura K, Masuzaki H, Nakayama S, Okai T, Kamei Y, et al.
    • Journal Title

      J Obstet Gynaecol Res

      Volume: 43 Issue: 8 Pages: 1245-1255

    • DOI

      10.1111/jog.13373

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Fetiform teratoma was a parthenogenetic tumour arising from a mature ovum2017

    • Author(s)
      Miura K, Kurabayashi T, Satoh C, Sasaki K, Ishiguro T, Yoshiura KI and Masuzaki H
    • Journal Title

      Journal of Human Genetics

      Volume: 印刷中 Issue: 9 Pages: 803-808

    • DOI

      10.1038/jhg.2017.45

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] 出生前診断-ゲノム医学からゲノム医療へ-2020

    • Author(s)
      三浦清徳
    • Organizer
      第72回日本産科婦人科学会・学術講演会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] HTLV-1母子感染2020

    • Author(s)
      三浦清徳
    • Organizer
      第90回日本感染症学会西日本地方会学術集会・第63回日本感染症学会中日本地方会学術集会・第68回日本化学療法学会西日本支部総会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] 周産期と生殖2019

    • Author(s)
      三浦清徳
    • Organizer
      第42回日本母体胎児医学会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] HBV,HCV, HTLV-12019

    • Author(s)
      三浦清徳
    • Organizer
      第71回日本産科婦人科学会学術講演会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] HTLV-1の母子感染抑止プロジェクト2019

    • Author(s)
      三浦清徳
    • Organizer
      第93回日本感染症学会総会・学術講演会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] 出生前診断の遺伝カウンセリング2018

    • Author(s)
      三浦清徳
    • Organizer
      第70回日本産科婦人科学会学術講演会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 遺伝性乳がん卵巣がん症候群について2018

    • Author(s)
      三浦清徳
    • Organizer
      第14回九州LBC研究会学術集会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 3.Clinical significances of C19MC and C14MC microRNAs in perinatal management2018

    • Author(s)
      三浦清徳
    • Organizer
      International Federation of Placenta Associations (IFPA)2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 生殖医療・出生前診断2018

    • Author(s)
      三浦清徳
    • Organizer
      日本人類遺伝学会第63回大会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 周産期カウンセリングにおける必須知識2018

    • Author(s)
      三浦清徳
    • Organizer
      第4回日本産科婦人科遺伝診療学会
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Identification of mesenchymal stem cells derived from human placental tissue2018

    • Author(s)
      Miura K, Fuchi N, Li TS, Masuzaki H
    • Organizer
      The 21th International Conference on Prenatal Diagnosis and Therapy
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 出生前診断における遺伝カウンセリング体制2017

    • Author(s)
      三浦清徳、増崎英明
    • Organizer
      第3回日本産科婦人科遺伝診療学会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 母体血を用いた胎児染色体検査で13トリソミー偽陽性であった1例2017

    • Author(s)
      三浦清徳、尾崎 守、長谷川ゆり、三浦生子、増崎英明
    • Organizer
      第25回日本胎盤学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] ガイドラインや見解で出生前診断はどう変わったか2017

    • Author(s)
      三浦清徳、増崎英明
    • Organizer
      第53回日本周産期・新生児医学会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 本邦における超音波検査による胎児診断の現状に関する調査2017

    • Author(s)
      三浦清徳、増崎英明
    • Organizer
      第90回日本超音波医学会学術集会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Decreased cell-free but not exosomal miR-518b in maternal plasma is caused by amniocentesis2017

    • Author(s)
      Miura K, Higashijima A, Miura S, Hasegawa Y, Masuzaki H
    • Organizer
      第69回日本産科婦人科学会学術講演会
    • Related Report
      2017 Research-status Report
  • [Book] Fetal Morph Functional Diagnosis. Chapter 22 Gene Disorders and Genetic Counseling2021

    • Author(s)
      Miura S and Miura K
    • Total Pages
      354
    • Publisher
      Springer
    • Related Report
      2020 Annual Research Report
  • [Remarks] 長崎大学産婦人科

    • URL

      https://www.med.nagasaki-u.ac.jp/gyneclgy/

    • Related Report
      2020 Annual Research Report
  • [Remarks] 長崎大学産婦人科ホームページ

    • URL

      http://www.med.nagasaki-u.ac.jp/gyneclgy/

    • Related Report
      2019 Research-status Report
  • [Remarks] 長崎大学医学部産婦人科ホームページ

    • URL

      http://www.med.nagasaki-u.ac.jp/gyneclgy/

    • Related Report
      2018 Research-status Report
  • [Remarks] 長崎大学医学部産婦人科

    • URL

      http://www.med.nagasaki-u.ac.jp/gyneclgy/

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2022-01-27  

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