The study for development of prediction method of placental adhesion, using cell-free DNA in maternal blood

• Project/Area Number: 17K11254
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Obstetrics and gynecology
• Research Institution: Showa University
• Principal Investigator: Koide Keiko 昭和大学, 医学部, 講師 (90384437)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: 癒着胎盤 / 産科学

Outline of Final Research Achievements

We have analyzed five DNA methylation profile sets of villous DNA and cell-free DNA from the women of the 5th day of postpartum to discover some genes which DNA methylation rate of cell-free DNA in the women of the 5th day of postpartum and of villous DNA are 0% and 100%, respectively.
Using blood from pregnant women in 32 weeks of gestation who had diagnosed with placenta previa,it will be verified whether placenta-derived cell-free DNA volume measurement can be a clinically applicable, pre-delivery adhesion placenta prediction method.

Academic Significance and Societal Importance of the Research Achievements

癒着胎盤は産科危機的出血の要因となり、その管理には高度な医療技術・施設が要求される。よって、癒着胎盤症例の予後を改善させる最大の方法は、分娩前に癒着胎盤を予測し適当な施設で周産期管理を行うことである。本研究により、癒着胎盤群と非癒着胎盤群とで母体血中cell-free DNA量が大きく異なる遺伝子を発見できれば、癒着胎盤群の予測精度の高いカットオフ値を設定することができる。これが実現できれば癒着胎盤の分娩前予測法として臨床診療での活用の可能性は高まり、「分娩前に癒着胎盤を予測し適当な施設で周産期管理を行う」という、癒着胎盤症例の理想的な周産期管理の実現に大きく寄与すると考える。