ion transport and its regulation in the inner ear (endolymphatic sac, stria vascularis)
| Project/Area Number |
17K11330
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Otorhinolaryngology
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| Research Institution | Kagawa University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 内耳 / イオン輸送 / 内リンパ嚢 / 血管条 / RT-PCR / リアルタイムPCR / メニエール病 / 内リンパ水腫 / イメージング / mRNA / LCM / ホルモン |
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| Outline of Final Research Achievements |
The endolymphatic sac in the inner ear is thought to absorb endolymph. Endolymphatic sac dysfunction causes an excess of endolymph, a pathological condition in Meniere's disease. A low-salt diet is an effective treatment for Meniere's disease.
We succeeded in extracting and quantifying a small amount of mRNA from the endolymphatic sac epithelium and succeeded in analyzing each portion of the endolymphatic sac. The expression of Na+/K+-ATPase, epithelial Na channel, and thiazide-sensitive Na-Cl cotransporter were significantly higher in the intermediate portion of the endolymphatic sac in the low-salt diet model animals than high-salt diet model animals. It is suggested that the low-salt diet may improve endolymphatic hydrops by activating endolymphatic absorption through activating Na transport in the endolymphatic sac, especially in the intermediate portion of the endolymphatic sac.
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| Academic Significance and Societal Importance of the Research Achievements |
減塩食・高塩食モデル動物を用いた本研究から、減塩食は内リンパ嚢における内リンパの吸収を促進し、内リンパ水腫を改善する可能性があることが示唆された。内リンパ水腫を病態とするメニエール病の治療の一つとして減塩食が勧められることを、動物モデルでも確認し、これまで臨床研究で想像されていた、内リンパ水腫の調節機構の一つを解明することができた。今後、さらに研究を進めることで、内リンパ水腫を病態とするメニエール病などの疾患の経験的治療の根拠を補強し、新たな治療法開発に結び付く可能性がある。
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Report
(4 results)
Research Products
(5 results)
