| Project/Area Number |
17K11520
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Pediatric surgery
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
宮原 克 順天堂大学, 医学(系)研究科(研究院), 技術員 (00420844)
藤原 なほ 順天堂大学, 医学部, 非常勤講師 (20589543)
岡和田 学 順天堂大学, 医学部, 非常勤講師 (60433778)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | ヒルシュスプルング病 / 臍帯由来幹細胞 / 腸管神経再生 / 細胞治療 / 再生医療 |
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| Outline of Final Research Achievements |
The umbilical cord is a rich source of stem and progenitor cells. We tried to establish the differentiation from umbilical cord stem cells (UCSCs) to enteric nervous system using SOX10-Venus mice. A preliminary experiment had shown that transplanted UCSCs were observed damaged bladder wall after 10 days intravenous injection. These findings suggested that UCSCs might regenerate to area of damaged bladder wall. We could not isolate UCSCs and establish of methods for the differentiation of UCSCs. Previous experiments by our group reported that transplanted SOX10-Venus mouse enteric neural crest-derived cells colonise the aganglionic colon in the Hirschsprung’s disease. Further study is needed to determine whether UCSCs differentiate and generate functioning neuronal networks as a new therapeutic for Hirschsprung’s disease.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究において、トランスジェニックマウスを用いることで臍帯由来幹細胞が障害組織に集積することが容易に観察され、他組織での再生に寄与する可能性も示唆された。今回我々が初めて臍帯由来幹細胞の腸管神経系への分化誘導を試行したため、新規治療法開発への足掛かりになることができたと考えている。
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