Examination of lymphangiogenesis effect on irradiated lymphatic endothelial cells by adipose tissue-derived stem cells.

• Project/Area Number: 17K11543
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: Nagasaki University
• Principal Investigator: YOSHIMOTO Hiroshi 長崎大学, 医歯薬学総合研究科(医学系), 准教授 (90513309)
• Co-Investigator(Kenkyū-buntansha): 大石 正雄 長崎大学, 病院(医学系), 助教 (90751230)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: リンパ管再生 / リンパ管内皮細胞 / 脂肪組織由来幹細胞 / 放射線照射 / 塩基性線維芽細胞成長因子 / 脂肪由来幹細胞 / 放射線障害 / リンパ管新生

Outline of Final Research Achievements

Irradiated human adipose tissue-derived stem cells (ADSC) have been found to promote cell proliferation, migration and luminogenesis of human cutaneous lymphatic endothelial cells. Irradiation promotes the secretion of basic fibroblast growth factor from ADSC, and is thought to activate vascular endothelial growth factor-C, which is considered to be the most important factor in promoting lymphatic endothelial cell proliferation and lymphangiogenesis.

Academic Significance and Societal Importance of the Research Achievements

本研究により、ヒト脂肪組織由来幹細胞はヒト皮膚リンパ管内皮細胞の細胞増殖能、遊走能および管腔形成能を促進し、さらにヒト脂肪組織由来幹細胞に放射線を照射すると効果がより高くなることが分かった。
放射線治療による四肢の二次性リンパ浮腫に対してヒト脂肪組織由来幹細胞が予防や治療に対して有効である可能性が示唆された。

Research Products

• [Journal Article] Paracrine Effects of Adipose-Derived Stem Cells Promote Lymphangiogenesis in Irradiated Lymphatic Endothelial Cells. (2019)
  • Author(s): Saijo H, Suzuki K, Yoshimoto H, Imamura Y, Yamashita S, Tanaka K.
  • Journal Title: Plast Reconstr Surg. Volume: 143(6) Issue: 6 Pages: 1189e-1200e
  • DOI: 10.1097/prs.0000000000005669
  • Peer Reviewed / Open Access
• [Presentation] Paracrine effect of adipose-derived stem cell promote lymphangiogenesis in irradiated lymphatic endothelial cells (2019)
  • Author(s): Hiroshi Yoshimoto, Hiroto Saijo, Yoshinobu Imamura, kasumi Tanaka
  • Organizer: 41st Annual Boswick Burn & Wound Symposium
  • Int'l Joint Research
• [Presentation] 放射線照射が脂肪組織由来幹細胞のリンパ管新生効果および成長因子分泌能に与える影響 (2018)
  • Author(s): 西條 広人、吉本 浩、今村 禎伸、田中 克己
  • Organizer: 第27回日本形成外科学会基礎学術集会
• [Presentation] 放射線照射が脂肪組織由来幹細胞のリンパ管新生効果に与える影響 (2017)
  • Author(s): 西條 広人、吉本 浩、今村 禎伸、田中 克己
  • Organizer: 日本形成外科学会基礎学術集会