Mechanism of tooth development based on mechanosensor

• Project/Area Number: 17K11616
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Morphological basic dentistry
• Research Institution: Kyushu University
• Principal Investigator: Wada Hiroko 九州大学, 歯学研究院, 助教 (70380706)
• Co-Investigator(Kenkyū-buntansha): 清島 保 九州大学, 歯学研究院, 教授 (20264054) ; 城戸 瑞穂 佐賀大学, 医学部, 教授 (60253457) ; 永田 健吾 九州大学, 歯学研究院, 助教 (90189134) ; 藤井 慎介 九州大学, 歯学研究院, 講師 (60452786) ; 長谷川 佳那 九州大学, 歯学研究院, 助教 (30793989)
• Project Period (FY): 2017-04-01 – 2021-03-31
• Project Status: Completed (Fiscal Year 2020)
• Budget Amount: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 歯 / 発生 / メカノセンサー / 歯胚

Outline of Final Research Achievements

Mechanosensitive ion channel Piezo1 might play an important role during development of tooth and periodontal tissue which function as receptors of mastication and occlusal pressure. In this study, we investigated to analyze an expression pattern and function of Piezo1 during the development of mouse lower first molars to clarify how Piezo1 is associated with tooth morphogenesis. As results, we indicated that Piezo1 mRNA showed a specific expression pattern in the developing tooth germ, and that Piezo1 might be involved in proliferation of both mDE6 and mouse tooth germ and in differentiation of ameloblasts in the tooth germ.

Academic Significance and Societal Importance of the Research Achievements

すべての臓器や器官は上皮間葉相互作用によって構成されており、歯の発生においても上皮間葉相互作用が極めて重要なステップであることが明らかになっている。「歯の再生医療」の実現のためには、上皮間葉相互作用を含む歯の発生メカニズムの解明が必須とされ、多数の研究室の取り組みにより多くの研究結果が報告されているが、未だ不明な点が多いのが現状である。本研究により、Piezo1が歯の形態形成に深く関わっていることを明らかにすることで、生物学的な意義だけでなく、歯の再生治療を含めた新規治療法の開発が期待され、「歯の再生医療」の実現に向けての意義が考えられる。

Research Products

• [Journal Article] The TRPV4-AKT axis promotes oral squamous cell carcinoma cell proliferation via CaMKII activation. (2020)
  • Author(s): Fujii S., Tajiri, Y., Hasegawa, K., Matsumoto,S., Yoshimoto, RU., Wada, H., Kishida, S., Kido, MA., Yoshikawa, H., Ozeki, S., Kiyoshima, T.
  • Journal Title: Lab Invest. Volume: 100(2) Issue: 2 Pages: 311-323
  • DOI: 10.1038/s41374-019-0357-z
  • NAID: 120006938538
  • Peer Reviewed / Open Access
• [Journal Article] Accelerated dentinogenesis by inhibiting the mitochondrial fission factor, dynamin related protein 1 (2018)
  • Author(s): Matsuishi Yumiko I.、Kato Hiroki、Masuda Keiji、Yamaza Haruyoshi、Hirofuji Yuta、Sato Hiroshi、Wada Hiroko、Kiyoshima Tamotsu、Nonaka Kazuaki
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 495 Issue: 2 Pages: 1655-1660
  • DOI: 10.1016/j.bbrc.2017.12.026
  • Peer Reviewed / Open Access
• [Presentation] A case of extramammary Paget's disease occuring in the hard palate of patient with oral squamous cell carcinoma (2018)
  • Author(s): Hiroko Wada, Shintaro Kawano, Seiji Nakamura and Tamotsu Kiyoshima
  • Organizer: 日本臨床口腔病理学会
• [Presentation] Expression and function of Piezo1 and Piezo2 in tooth germ development (2018)
  • Author(s): Misaki Abe, Hiroko Wada, Tamotsu Kiyoshima
  • Organizer: Kyudai Oral Bioscience 2018
  • Int'l Joint Research
• [Presentation] A case of Plasmablastic lymphoma arising in oral cavity and extraoral site (2017)
  • Author(s): Hiroko Wada, Koki Nagano, Yurie Mikami, Misaki Abe, Tomohiro Yamada, Yoshihide Mori and Tamotsu Kiyoshima
  • Organizer: 日本臨床口腔病理学会