| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Outline of Final Research Achievements |
We developed a method of bioluminescence imaging to visualize protein secretion by using Gaussia luciferase (GLase), and oscillatory insulin secretion was studied by our imaging method. A new pancreatic beta cell line stably expressing a fusion protein of insulin to GLase (Insulin-GLase), name iGL, was established. Synchronous insulin secretion from 2D- and 3D-cultured iGL cells was visualized by our bioluminescence imaging method. We also improved a method to determine the relative amount of insulin secretion from iGL cells with a luminometer. Thus, we provided useful tools and methods to investigate the mechanism of insulin secretion and diabetes.
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