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Development of pulp capping material based on the wound healing mechanism of the pulp tissue using bioinformatics

Research Project

Project/Area Number 17K11704
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Conservative dentistry
Research InstitutionOsaka University

Principal Investigator

TAKAHASHI YUSUKE  大阪大学, 歯学部附属病院, 講師 (60397693)

Co-Investigator(Kenkyū-buntansha) 石本 卓也  大阪大学, 工学研究科, 准教授 (50508835)
岡本 基岐  大阪大学, 歯学研究科, 助教 (60755354)
小道 俊吾  大阪大学, 歯学研究科, 特任研究員 (40804456)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords歯学 / 歯髄 / 覆髄 / バイオインフォマティクス / 創傷治癒
Outline of Final Research Achievements

This research aimed to develop a biological pulp capping material which could promote the wound healing process of dentin-pulp complex. We already discovered some proteins which promoted tertiary dentin formation in vivo. Bioinformatic analysis in silico was performed to compare the sequential alignment among protein S100 family proteins or among some mammalian species. As a result, we found some functional domains from the full-length proteins. Then, peptide array analysis was performed to investigate which peptide showed promotive effects on ALP activity of dental pulp cells and we could extract some specific peptides which revealed positive effects. Now, we are performing in vivo pulp capping experiments to investigate which peptide is the most suitable or critical for the wound healing process of dentin-pulp complex as a novel pulp capping material after cavity preparation using rat molars.

Academic Significance and Societal Importance of the Research Achievements

本研究により、これまでの歯科臨床に置いて存在しなかった歯髄の創傷治癒機転に基づく生物学的覆髄材の開発に向けて、大きな科学的知見が得られた。覆髄時に第三象牙質の形成を誘導する効果をもつタンパク質のアミノ酸配列の中から機能的役割を担うペプチド化合物を得て、覆髄材へと応用しようとする試みはこれまでに報告がなく、その学術的意義は非常に高いと考えられる。また、本研究成果を基に新しい覆髄材が開発されたら、歯髄保存の可能性がこれまでよりも向上するとともに、歯の寿命の延長の可能性も上昇し、ひいては健康寿命の延伸へもつながる可能性があり、非常に社会的意義も大きいと考えている。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (26 results)

All 2020 2019 2018 2017

All Journal Article (7 results) (of which Int'l Joint Research: 5 results,  Peer Reviewed: 7 results,  Open Access: 4 results) Presentation (17 results) (of which Int'l Joint Research: 8 results,  Invited: 1 results) Patent(Industrial Property Rights) (2 results) (of which Overseas: 1 results)

  • [Journal Article] Lithium-containing surface pre-reacted glass fillers enhance hDPSC functions and induce reparative dentin formation in a rat pulp capping model through activation of Wnt/β-catenin signaling2019

    • Author(s)
      Ali Manahil、Okamoto Motoki、Komichi Shungo、Watanabe Masakatsu、Huang Hailing、Takahashi Yusuke、Hayashi Mikako
    • Journal Title

      Acta Biomaterialia

      Volume: 96 Pages: 594-604

    • DOI

      10.1016/j.actbio.2019.06.016

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Protein S100-A7 Derived from Digested Dentin Is a Critical Molecule for Dentin Pulp Regeneration2019

    • Author(s)
      Komichi Shungo、Takahashi Yusuke、Okamoto Motoki、Ali Manahil、Watanabe Masakatsu、Huang Hailing、Nakai Takeo、Cooper Paul、Hayashi Mikako
    • Journal Title

      Cells

      Volume: 8 Issue: 9 Pages: 1002-1002

    • DOI

      10.3390/cells8091002

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Surface pre-reacted Glass filler contributes to tertiary dentin Formation through a Mechanism different than that of hydraulic calcium-silicate cement.2019

    • Author(s)
      Okamoto M, Ali M, Komichi S, Watanabe M, Huang H, Ito Yuki, Miura J, Hirose Y, Mizuhira M, Takahashi Y, Okuzaki D, Kawabata S, Imazato S, Hayashi M.
    • Journal Title

      Journal of Clinical Medicine.

      Volume: 8 Issue: 9 Pages: 11-11

    • DOI

      10.3390/jcm8091440

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of tissue inhibitor of metalloprotease 1 on human pulp cells in vitro and rat pulp tissue in vivo.2019

    • Author(s)
      Okamoto Motoki、Takahashi Yusuke、Komichi Shungo、Ali Manahill、Watanabe Masakatsu、Hayashi Mikako
    • Journal Title

      International Endodontic Journal

      Volume: ー Issue: 7 Pages: 1051-1062

    • DOI

      10.1111/iej.13099

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dentinogenic effects of extracted dentin matrix components digested with matrix metalloproteinases2018

    • Author(s)
      Okamoto Motoki、Takahashi Yusuke、Komichi Shungo、Cooper Paul R.、Hayashi Mikako
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 10690-10690

    • DOI

      10.1038/s41598-018-29112-3

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Application of a direct pulp capping cement containing S-PRG filler2018

    • Author(s)
      Takahashi Yusuke、Okamoto Motoki、Komichi Shungo、Imazato Satoshi、Nakatsuka Toshiyuki、Sakamoto Shuji、Kimoto Katsuya、Hayashi Mikako
    • Journal Title

      Clinical Oral Investigations

      Volume: 23 Issue: 4 Pages: 1723-1731

    • DOI

      10.1007/s00784-018-2596-6

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel evaluation method of dentin repair by direct pulp capping using high-resolution micro-computed tomography2018

    • Author(s)
      Okamoto Motoki、Takahashi Yusuke、Komichi Shungo、Ali Manahil、Yoneda Naomichi、Ishimoto Takuya、Nakano Takayoshi、Hayashi Mikako
    • Journal Title

      Clinical Oral Investigations

      Volume: - Issue: 8 Pages: 1-9

    • DOI

      10.1007/s00784-018-2374-5

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] The role of mTOR signal on Tertiary dentin formation2020

    • Author(s)
      Okamoto M, Takahashi Y, Ali M, Watanabe M, Huang H, Matsumoto S, Kuriki N, Komichi S, Hayashi M
    • Organizer
      98th General Session & Exhibition of the IADR
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Evaluation of Inflammatory Response During Wound Healing Process In Caries Affected Dental Pulp2020

    • Author(s)
      Huang H, Okamoto M, Ali M, Watanabe M, Matsumoto S, Takahashi Y, Komichi S, Hayashi M
    • Organizer
      98th General Session & Exhibition of the IADR
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Bioactivity and Biocompatibility of Lithium Contained Surface Pre-reacted Glass Fillers2019

    • Author(s)
      Ali M, Okamoto M, Komichi S, Watanabe M, Huang H, Takahashi Y, Hayashi M
    • Organizer
      97th General Session & Exhibition of the IADR
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The role of mTOR signal on reactionary and reparative dentin formation in vivo2019

    • Author(s)
      Okamoto M, Takahashi Y, Komichi S, Ali M, Watanabe M, Huang H, Cooper P, Hayashi M
    • Organizer
      IADR Pulp Biology and Regeneration Group Satellite Symposium
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 生物学的覆髄材料の臨床的評価を目指したラット覆髄モデルの開発2019

    • Author(s)
      岡本基岐, 高橋雄介, 黄 海玲, 小道俊吾, S. Ali Manahil, 渡邉昌克, 松本紗也子, 林 美加子
    • Organizer
      第151回日本歯科保存学会2019年度秋季学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 生物学的根拠に基づくペプチド覆髄剤の開発2019

    • Author(s)
      高橋雄介, 渡邉昌克, 岡本基岐, 小道俊吾, S. Ali Manahil, 黄 海玲, 松本紗也子, 林 美加子
    • Organizer
      第151回日本歯科保存学会2019年度秋季学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Designing a new pulp capping material: from fundamental data to material development2019

    • Author(s)
      Takahashi Y
    • Organizer
      25th European Dental Materials Conference
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 歯髄創傷治癒過程におけるProtein S100-A7の局在解析2018

    • Author(s)
      小道俊吾, 高橋雄介, 岡本基岐, S Ali Manahil, 渡邉昌克, 林 美加子
    • Organizer
      第148回日本歯科保存学会2018年度春季学術大会
    • Related Report
      2018 Research-status Report
  • [Presentation] Biocompatibility of S-PRG filler cements in rat subcutaneous implantation model2018

    • Author(s)
      Okamoto M, Ali Manahil, Komichi S, Watanabe M, Naruse H, Ito Y, Takahashi Y, Hayashi M
    • Organizer
      96th General Session & Exhibition of the IADR
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Lithium contained S-PRG cement facilitated hDPSCs migration and functional vascularity2018

    • Author(s)
      Ali M, Okamoto M, Komichi S, Watanabe M, Takahashi Y, Hayashi M
    • Organizer
      96th General Session & Exhibition of the IADR
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Lithium containing S-PRG fillers enhanced tertiary dentin formation via Wnt/β-catenin pathway activation2018

    • Author(s)
      Ali M, Okamoto M, Komichi S, Watanabe M, Takahashi Y, Hayashi M
    • Organizer
      IFEA 11th World Endodontic Congress
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 象牙質-歯髄複合体の創傷治癒を促進する象牙質基質分解産物の同定と機能解析2017

    • Author(s)
      小道俊吾, 高橋雄介, 岡本基岐, S. Ali Manahil, 林 美加子
    • Organizer
      第146回日本歯科保存学会2017年度春季学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] リチウムイオン含有S-PRGセメントの直接覆髄剤としての応用2017

    • Author(s)
      S. Ali Manahil, 岡本 基岐, 小道 俊吾, 高橋 雄介, 伊藤 善博, 林 美加子
    • Organizer
      第146回日本歯科保存学会2017年度春季学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 高解像度Micro-CTを用いた覆髄剤より誘導された第三象牙質の定量定性評価2017

    • Author(s)
      岡本 基岐, 小道 俊吾, 高橋 雄介, Manahil Saeed, 米田 直道, 石本 卓也, 中野 貴由, 林 美加子
    • Organizer
      第37回日本骨形態計測学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Er:YAG laserの歯髄保存療法における有効性の検討2017

    • Author(s)
      岡本 基岐, 小道 俊吾, 伊藤 勇紀, 栗木 菜々子, 渡邉 昌克, 高橋 雄介, 林 美加子
    • Organizer
      第29回日本レーザー歯学会総会・学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] リチウムイオン含有S-PRGセメントが歯髄細胞に与える影響2017

    • Author(s)
      S. Ali Manahil、岡本 基岐、小道 俊吾、渡邉 昌克、伊藤 善博、高橋 雄介、林 美加子
    • Organizer
      第147回日本歯科保存学会2017年度秋季学術大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 低酸素誘導因子(HIF-1a)による第三象牙質形成に与える影響2017

    • Author(s)
      岡本基岐, 小道俊吾, S. Ali Manahil, 渡邉昌克, 高橋雄介, 林 美加子
    • Organizer
      第147回日本歯科保存学会2017年度秋季学術大会
    • Related Report
      2017 Research-status Report
  • [Patent(Industrial Property Rights)] 歯科用組成物2019

    • Inventor(s)
      岡本基岐、高橋雄介、林 美加子他
    • Industrial Property Rights Holder
      岡本基岐、高橋雄介、林 美加子他
    • Industrial Property Rights Type
      特許
    • Filing Date
      2019
    • Related Report
      2019 Annual Research Report
    • Overseas
  • [Patent(Industrial Property Rights)] 象牙質-歯髄組織を再生させる新規直接覆髄材料2018

    • Inventor(s)
      岡本基岐、高橋雄介、林美加子他
    • Industrial Property Rights Holder
      岡本基岐、高橋雄介、林美加子他
    • Industrial Property Rights Type
      特許
    • Industrial Property Number
      2018-205226
    • Filing Date
      2018
    • Related Report
      2018 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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