| Project/Area Number |
17K11730
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Conservative dentistry
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| Research Institution | Chiba Prefectural University of Health Sciences (2018-2020)
Kyushu University of Nursing and Social Welfare (2017) |
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Principal Investigator |
Ishikawa Yuko 千葉県立保健医療大学, 健康科学部, 教授 (40401757)
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| Co-Investigator(Kenkyū-buntansha) |
大島 勇人 新潟大学, 医歯学系, 教授 (70251824)
中富 満城 九州歯科大学, 歯学部, 講師 (10571771)
斎藤 浩太郎 新潟大学, 医歯学系, 助教 (10733719)
依田 浩子 新潟大学, 医歯学系, 准教授 (60293213)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 歯髄 / ソニック・ヘッジホッグシグナル / 静的幹細胞 / Gliタンパク / Ptch1受容体 / マウス / 発生 / Shhシグナル / Gliたんぱく / 歯学 / 細胞・組織 / 発生・分化 |
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| Outline of Final Research Achievements |
In molars, dense H2B-GFP-label-retaining cells (H2B-GFP-LRCs) were densely distributed throughout the dental pulp during P1 to postnatal week 2 (P2W) and decreased in number by postnatal P3W, whereas the number of dense H2B-GFP-LRCs in the subodontoblastic layer increased in number at P2W. Gli1+ and Ptch1+ cells were distributed throughout the enamel organ and dental pulp, including the odontoblast and subodontoblastic layers. In incisors, the apical bud contained H2B-GFP-LRCs, Gli1+ cells, and Ptch1+ cells. The addition of Shh antibody to explants induced a decrease in the number of Sox2+ cells due to the increase in apoptotic cells in the apical bud.
Thus, the Shh-Ptch-Gli signaling pathway plays a role in maintaining quiescent adult stem cells and regulating the function of odontoblasts.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、ShhレセプターであるPtch1・2の異なる発現パターンに着目し、Shhシグナルと静的幹細胞維持機構との関連を解明したことは、Shhの新たな役割の解明に繋がった。また、マウス切歯および臼歯発生過程における静的幹細胞維持とShhシグナルとの関連を解明したことから、静的幹細胞を用いた歯髄再生療法への展開に貢献できる。
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