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Functional analysis of chondroitin sulfate consists of osseointegratoin

Research Project

Project/Area Number 17K11802
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Dental engineering/Regenerative dentistry
Research InstitutionNagoya University

Principal Investigator

Shuhei Tsuchiya  名古屋大学, 医学部附属病院, 助教 (20569785)

Co-Investigator(Kenkyū-buntansha) 黒田 健介  名古屋大学, 未来材料・システム研究所, 准教授 (00283408)
Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsオッセオインテグレーション / コンドロイチン4硫酸 / 骨髄由来間葉系幹細胞 / プロテオグリカン / 骨芽細胞 / 歯科インプラント / インプラント / 糖鎖 / 歯学 / タンパク質
Outline of Final Research Achievements

Human marrow mesenchyma stem cell which was controlled Chondroitin 4-O-Sulfotransferase 1 (C4ST-1) gene expression by the gene silencing method(sh-BMSCs) was used in this study and cultured it on a titanium disc.
sh-BMSCs has character of stem cell. After culturing sh-BMSCs on a titanium disc one and two weeks, cell proliferation and osteogenic differentiation was not significant difference. After culturing sh-BMSCs for two weeks, and observing the proteoglycan layer which intervened between titanium and BMSCs, the area of proteoglycan was decreased. Gene expression of IDO of sh-BMSCs which I cultured with a titanium board and IL-6 decreased in comparison with BMSCs. In addition, the growth factor which sh-BMSCs which cultured with a titanium disc secreted reduced gene expression of the M1 macrophage inflammation-shaped with a macrophage.
C4ST-1 in BMSCs on titanium play an important role of proteoglycan layer on titanium, it makes affect immune tolerance ability.

Academic Significance and Societal Importance of the Research Achievements

本研究の意義は、チタン上に形成されるプロテオグリカン層の機能の1つを明らかにしたことである。これは、歯科インプラント学の最重要事項であるオッセオインテグレーションの基本原理の一部を解明することができた。
本研究の重要性は、生体材料の周囲で引き起こされている生物学的な反応を明らかにすることによって、チタンのみではなく、臨床で使われている生体材料の安全性および効能効果の評価項目に応用することができる。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (21 results)

All 2019 2018 2017 Other

All Journal Article (6 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 6 results,  Open Access: 5 results) Presentation (12 results) (of which Invited: 1 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Design of a Randomized Controlled Clinical Study of tissue-engineered osteogenic materials using bone marrow-derived mesenchymal cells for Maxillomandibular bone defects in Japan: the TEOM study protocol2019

    • Author(s)
      Shimizu Shinobu、Tsuchiya Shuhei、Hirakawa Akihiro、Kato Katsuyoshi、Ando Masahiko、Mizuno Masaaki、Osugi Masashi、Okabe Kazuto、Katagiri Wataru、Hibi Hideharu
    • Journal Title

      BMC Oral Health

      Volume: 19 Issue: 1 Pages: 69-69

    • DOI

      10.1186/s12903-019-0753-1

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] 先天性無フィブリノゲン血症患者の抜歯術における周術期管理を行った1例2019

    • Author(s)
      岡部一登,土屋周平,酒井 陽,松下嘉泰,杉本圭佑,日比英晴
    • Journal Title

      日本口腔外科学会雑誌

      Volume: 65 Issue: 8 Pages: 507-512

    • DOI

      10.5794/jjoms.65.507

    • NAID

      130007731829

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A Mouse Distraction Osteogenesis Model2018

    • Author(s)
      Fujio Masahito、Osawa Yusuke、Matsushita Masaki、Ogisu Kota、Tsuchiya Shuhei、Kitoh Hiroshi、Hibi Hideharu
    • Journal Title

      Journal of Visualized Experiments

      Volume: 141 Issue: 141 Pages: 0-0

    • DOI

      10.3791/57925

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Ophthalmoplegia considered to be Tolosa-Hunt syndrome: A case report2018

    • Author(s)
      Okabe Kazuto, Kaneko Ryuji, Kawai Takamasa, Mochizuki Daisuke, Tsuchiya Shuhei, HIbi Hideharu
    • Journal Title

      JOURNAL OF ORAL AND MAXILLOFACIAL SURGERY MEDICINE AND PATHOLOGY

      Volume: 30 Issue: 5 Pages: 461-465

    • DOI

      10.1016/j.ajoms.2018.04.001

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Kaempferol-immobilized titanium dioxide promotes formation of new bone: effects of loading methods on bone marrow stromal cell differentiation in vivo and in vitro2018

    • Author(s)
      S. Tsuchiya, K. Sugimoto, H. Kamio, K. Okabe, K. Kuroda, M. Okido, H. Hibi
    • Journal Title

      International Journal of Nanomedicine

      Volume: 13 Pages: 1665-1676

    • DOI

      10.2147/ijn.s150786

    • Related Report
      2018 Research-status Report 2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Osteoradionecrosis of the jaw caused by periapical periodontitis: a case report2017

    • Author(s)
      Keisuke Sugimoto, Shuhei Tsuchiya, Kenji Hara, Yoshihiro Matsushita, Masahito Fujio, Hideharu Hibi
    • Journal Title

      Oral and Maxillofacial Surgery, Medicine and Pathology

      Volume: 29 Issue: 4 Pages: 328-333

    • DOI

      10.1016/j.ajoms.2017.02.002

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] オッセオインテグレーションを構成するコンドロイチン4硫酸の機能解析2019

    • Author(s)
      神尾尚伸、土屋周平、黒田健介、興戸正純、外山直人、藤尾正人、日比英晴
    • Organizer
      第64回日本口腔外科学会総会・学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] コンドロイチン4硫酸転移酵素―1が頭頸部扁平上皮癌に与える影響2019

    • Author(s)
      土屋周平、神尾尚伸、太田優也、外山直人、日比英晴
    • Organizer
      第64回日本口腔外科学会総会・学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] TNF-α刺激歯根膜由来間葉系幹細胞が頭頸部扁平上皮癌に対する抗腫瘍効果2019

    • Author(s)
      小原豪、土屋周平、太田優也、神尾尚伸、岡部一登、日比英晴
    • Organizer
      第64回日本口腔外科学会総会・学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 大気圧プラズマ処理したチタンがマクロファージの極性変化と骨形成に与える影響2019

    • Author(s)
      外山直人、土屋周平、黒田健介、藤尾正人、岡部一登、荻須宏太、神尾尚伸、日比英晴
    • Organizer
      第64回日本口腔外科学会総会・学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Le For Ⅰ型骨切り術の固定法と術後の骨片変位量の検討2019

    • Author(s)
      藤尾正人、佐世暁、荻須宏太、土屋周平、酒井陽、日比英晴
    • Organizer
      第64回日本口腔外科学会総会・学術大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] TNF-α刺激歯髄由来間葉系幹細胞はTRAILを介して口腔扁平上皮癌を抑制する2019

    • Author(s)
      太田優也、土屋周平、神尾尚伸、岡部一登、日比英晴
    • Organizer
      第43回日本頭頸部癌学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 伸展刺激下で得た細胞培養上清における骨形成・血管新生能の検討2019

    • Author(s)
      荻須宏太、藤尾正人、土屋周平、岡部一登、外山直人、日比英晴
    • Organizer
      第73回日本口腔科学会学術集会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 伸展刺激下で得た細胞培養上清における骨形成・血管新生能の検討2019

    • Author(s)
      荻須宏太,藤尾正人,土屋周平,岡部一登,外山直人,日比英晴
    • Organizer
      第73回日本口腔科学会
    • Related Report
      2018 Research-status Report
  • [Presentation] Le Fort I 型骨切り術の固定法と術後安定性の検討2018

    • Author(s)
      藤尾正人,佐世暁,荻須宏太,土屋周平,酒井陽,日比英晴
    • Organizer
      第63回日本口腔外科学会
    • Related Report
      2018 Research-status Report
  • [Presentation] チタン表面の大気圧プラズマ処理がマクロファージの挙動におよぼす影響の検討2018

    • Author(s)
      外山直人,土屋周平,原憲史,藤尾正人,岡部一登,佐世暁,荻須宏太,神尾尚伸,日比英晴
    • Organizer
      第63回日本口腔外科学会
    • Related Report
      2018 Research-status Report
  • [Presentation] 伸展刺激下で得た細胞培養上清における骨形成能の検討2018

    • Author(s)
      荻須宏太,藤尾正人,土屋周平,神尾尚伸,外山直人,日比英晴
    • Organizer
      第63回日本口腔外科学
    • Related Report
      2018 Research-status Report
  • [Presentation] 骨髄由来間葉系細胞による顎骨再生療法の評価と臨床応用2018

    • Author(s)
      土屋周平、清水忍、片桐渉
    • Organizer
      第17回日本再生医療学会総会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Book] The Sinus Bone Graft, Third Edition / Tissue-Engineered Bone and Cell-Conditioned Media2019

    • Author(s)
      Hibi H, Katagiri W, Tsuchiya S, Omori M, Ueda M
    • Total Pages
      288
    • Publisher
      Quintessence Publishing Co Inc
    • ISBN
      9780867157918
    • Related Report
      2019 Annual Research Report
  • [Remarks] 名古屋大学医学部附属病院歯科口腔外科

    • URL

      https://zzzgundam2008.wixsite.com/nu-oandm-surgery

    • Related Report
      2018 Research-status Report
  • [Remarks] 名古屋大学教員データシステム

    • URL

      http://profs.provost.nagoya-u.ac.jp/view/html/100001602_ja.html

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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