| Project/Area Number |
17K11813
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | The Nippon Dental University |
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Principal Investigator |
Xiao Li 日本歯科大学, 生命歯学部, 講師 (80548256)
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| Co-Investigator(Kenkyū-buntansha) |
佐伯 周子 日本歯科大学, 生命歯学部, 准教授 (90281435)
岡村 尚 日本歯科大学, 生命歯学部, 助教 (80795154)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 神経再生 / アルツハイマー病 / ヒト歯髄幹細胞 / 酸化ストレス / アミロイドβ / 酸化ストレス耐性 / ヒト乳歯歯髄細胞 / 神経保護 / 培養脳スライス / 神経細胞新生 / 海馬組織 / ヒト歯髄由来幹細胞 / 中枢神経再生 / 再生医学 / 細胞組織 / 神経科学 / 老化 |
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| Outline of Final Research Achievements |
This study first reconstructed an in vitro three-dimensional model of Alzheimer's disease. Oxidative stress-tolerant stem cells from human exfoliated deciduous teeth (OST-SHED) were created by H2O2-stimulation. OST-SHED could protect the brain tissue from oxidative stress, reduce the accumulation of amyloid β (Aβ), and increase activity of Neprilysin (one of the Aβdegrading enzymes). Although OST-SHED had lower cell viability, the neuronal protection
of OST-SHED was significantly superior to that of normal SHED. Our findings suggest that SHED, especially OST-SHED, could prevent oxidative stress induced brain damage and improve the pathology of Alzheimer's disease. OST-SHED can be explored as a new therapeutic tool for IBD, TBI and neurodegenerative disorders.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究は初めてIn vitroにおいてアルツハイマー病の病態モデルを構築し、酸化ストレス耐性歯髄幹細胞の開発に成功した。この病態モデルと酸化ストレス耐性歯髄幹細胞を用いて、神経再生、抗酸化、Aβクリアランス促進、抗老化を総合的に行う治療法を開発し、世界に先駆けて認知症を始めとする神経変性疾患の根本的な治療法を開発することにつながると考えられる。
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