| Project/Area Number |
17K11848
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Oita University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
松尾 哲孝 大分大学, 医学部, 准教授 (10284788)
佐々木 隆子 大分大学, 医学部, 客員研究員 (30133193)
矢野 博之 大分大学, 医学部, 客員研究員 (50448552)
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| Project Period (FY) |
2017-04-01 – 2023-03-31
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| Project Status |
Completed (Fiscal Year 2022)
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| Budget Amount *help |
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | コラーゲン発現 / 非コードRNA / マイクロRNA / 長鎖非コードRNA / 硬組織 / 細胞外マトリックス / circ（環状）RNA / コラーゲン遺伝子 / 遺伝子発現 / 遺伝子発現調節 / コラーゲン / 転写因子 / 微小環境 |
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| Outline of Final Research Achievements |
Collagens are major constituents of microenvironment of the hard tissues such as the bone and the dentin. Type I collagen with other minor type of collagens forms the collagen fibrils to perform cell proliferation and migration of the osteoblasts and the odontoblasts. The gene expression is regulated in the many steps. We have mainly investigated the transcriptional regulation of the collagen genes. In the present study, we examined translational gene regulation. Non-coding-protein RNAs, microRNA, long non-coding RNA, and circular RNA, regulate the gene expression. MicroRNAs are approximately 20-25 nucleotides in length and act as negative regulators of target genes. The results showed that miR-29b regulates the Col5a1 gene expression through binding to the 3’UTR in the osteoblasts. lncRNAs, which are several hundred nucleotides in length, are also regulate the gene expression. The expression of type I collagen was thought to be regulated by a couple of lncRNAs.
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| Academic Significance and Societal Importance of the Research Achievements |
コラーゲンは体タンパクの約３０％を占め、特に硬組織の主成分であり、コラーゲン遺伝子からコラーゲン線維形成までのメカニズムを解明することは極めて重要である。コラーゲンは骨や歯の強度に直接関与するものであり、高齢化社会を迎え、骨粗鬆症や歯牙の劣化や喪失等が医療において問題視されるなかで、本研究はこれらの再生医療につながる研究である。
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