Identification of osteoclast-supporting cells and the investigation of the in vivo functions of the cells using mouse ES cells
| Project/Area Number |
17K11898
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
Okayasu Mari 東京大学, 医学部附属病院, 助教 (10610941)
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| Co-Investigator(Kenkyū-buntansha) |
大庭 伸介 東京大学, 大学院医学系研究科(医学部), 准教授 (20466733)
北條 宏徳 東京大学, 大学院医学系研究科(医学部), 准教授 (80788422)
菅家 康介 東京大学, 医学部附属病院, 登録診療員 (90779810)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
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| Keywords | 骨代謝 / ES細胞 / 骨芽細胞 / 破骨細胞 / ＥＳ細胞 |
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| Outline of Final Research Achievements |
To better understand molecular mechanisms underlying osteoblast-mediated osteoclast functions, we developed a three dimensional differentiation protocol to direct from mouse ES cells to osteoblasts and osteocytes. We confirmed that bone marrow-derived cells differentiated into osteoclasts under co-culture with the ES cells-directed osteoblasts and osteocytes. We further performed gene expression analysis in stepwise differentiation stages from the ES cells to osteoblasts and osteocytes in order to identify novel cell-populations and factors contributing to osteoclast differentiation. These results provided insights into establishments of in vitro bone remodeling model and candidates of factors regulating osteoclast differentiation.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究で確立したマウスES細胞の三次元分化誘導法は、骨芽細胞だけでなく骨細胞への分化誘導を可能にした。これにより、骨芽細胞・骨細胞と破骨細胞のリモデリングをin vitroで再現できる可能性が示された。さらに本研究で得られた新規破骨細胞制御因子の候補は、将来的に研究が進みその効果が実証された場合は、新たな骨疾患治療薬の候補となる可能性を秘めている。
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Report
(4 results)
Research Products
(3 results)
