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Regulatory Mechanisms of Bronchial Asthma and Tracheal Epithelial Stabilization by Phytochemicals

Research Project

Project/Area Number 17K11899
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeMulti-year Fund
Section一般
Research Field Surgical dentistry
Research InstitutionTokyo Medical and Dental University

Principal Investigator

Wakita Ryo  東京医科歯科大学, 大学院医歯学総合研究科, 准教授 (60376712)

Co-Investigator(Kenkyū-buntansha) 船山 ひろみ  鶴見大学, 歯学部, 講師 (00359530)
Project Period (FY) 2017-04-01 – 2022-03-31
Project Status Completed (Fiscal Year 2021)
Budget Amount *help
¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2019: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywordsフィトケミカル / 気管支平滑筋 / kushenol / カルシウムイオン / 気管上皮 / 気管支喘息 / カルシウムチャネル
Outline of Final Research Achievements

Pretreatment of bronchial smooth muscle cells with kushenol, a compound of Sophora flavescens, resulted in inhibition of intracellular calcium ion concentrations, but suppression of prostaglandin receptors did not attenuate this effect. Considering studies using other herbal herb-derived ingredients, we focused on phosphokinase A (PKA) activation in bronchial smooth muscle cells treated with supernatant of tracheal epithelial cells that were exposed to kushenol, but we did not find any obvious effect. As a guide to PKA activation, we have changed the target to other proteins to confirm the results, but it is also possible that the pathways mediated by phosphokinase C or inositol 3-phosphate are being inhibited. We are planning to examine calcium ion concentrations in the sarcoplasmic reticulum as well as PLC activity.

Academic Significance and Societal Importance of the Research Achievements

漢方に使用される生薬はハーブとともに代替医療の有力な候補とされており、その薬理効果の解析にも注目が集まっている。その一つである苦参には上皮・粘膜抗炎症作用の報告があり、苦参を含む生薬合剤は気管支収縮を抑制することが報告されている。苦参のフィトケミカルであるkushenolの気管支平滑筋に対する直接作用は判明したが、その機序および気管上皮を介した間接作用は既知の生薬成分とは異なり未だ検索の途中にある。本研究の意義は、気管支収縮への調節機構が判明することで、従来は症状の安定化が困難だった気管支喘息への新しい選択肢となりうることにある。

Report

(6 results)
  • 2021 Annual Research Report   Final Research Report ( PDF )
  • 2020 Research-status Report
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (1 results)

All 2019

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Open Access: 1 results)

  • [Journal Article] Effects of Zoledronate on Local and Systemic Production of IL-1β, IL-18, and TNF-α in Mice and Augmentation by Lipopolysaccharide2019

    • Author(s)
      Funayama Hiromi、Tashima Itaru、Okada Satoru、Ogawa Takuya、Yagi Hideki、Tada Hiroyuki、Wakita Ryo、Asada Yoshinobu、Endo Yasuo
    • Journal Title

      Biological and Pharmaceutical Bulletin

      Volume: 42 Issue: 6 Pages: 929-936

    • DOI

      10.1248/bpb.b18-00923

    • NAID

      130007657675

    • ISSN
      0918-6158, 1347-5215
    • Year and Date
      2019-06-01
    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access

URL: 

Published: 2017-04-28   Modified: 2023-01-30  

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