| Project/Area Number |
17K11961
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontics/Pediatric dentistry
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| Research Institution | Okayama University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
仲野 道代 (松本道代) 岡山大学, 医歯薬学総合研究科, 教授 (30359848)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | Streptococcus mutans / Gbpc / バイオインフォマティクス / グルカン結合タンパクC / グルカン結合タンパク / GbpC / Gbp / 齲蝕 / う蝕 |
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| Outline of Final Research Achievements |
Streptococcus mutans, a major pathogenic bacterium involved in development of dental caries, produces multiple glucan-binding proteins (Gbps) that bind to glucan and other molecules. In our previous study, bioinformatics analysis results identified a glucan-binding domain (GBD) located in the middle of the gbpC encoding GbpC. In addition, an MT8148 strain deficient of GBD was constructed to examine adherence to human umbilical vein epithelial cells (HUVECs). Adhesion to HUVECs by the deficient strain was reduced as compared to that of the parental strain. In this study, we examined the inhibitory effects of an antibody against the peptide encoded by GBD on induction of dental caries using a rat caries model. The caries score in the group that receive adminisration of the antibody was decreased as compared to that of control group. These results suggest that inhibition of the function of the binding domain of GbpC is effective to prevent its invovlvement in dental caries development.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では、バイオインフォマティクスの手法を用いて齲触原性細菌 Streptococcus mutans の表層タンパクの１つであるグルカン結合タンパク GbpC の立体構造からグルカン結合領域を明らかにし、その領域の配列をもとに抗ペプチド抗体を作製した。さらにラット齲蝕実験系において、ラット口腔内に抗ペプチド抗体の直接投与した実験群では、投与しない群と比較して齲蝕抑制効果が認められた。このペプチドの抗体産生誘導の検討や安全性の高いアジュバンドを必要とするなど課題は多いが、実現すればヒトが長年にわたって悩まされている齲蝕を減少させるワクチンの開発へと繋がる可能性がある。
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