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Structural analyses of "abnormal" structures of histone proteins induced by DNA damage using synchrotron radiation circular dichroism spectroscopy

Research Project

Project/Area Number 17K12825
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Risk sciences of radiation and chemicals
Research InstitutionHiroshima University

Principal Investigator

Izumi Yudai  広島大学, 放射光科学研究センター, 助教 (20595772)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
KeywordsDNA損傷応答 / ヒストン / 円二色性 / 翻訳後修飾 / 放射光 / 放射線生物学 / DNA損傷
Outline of Final Research Achievements

This work revealed that the global structural changes in histone H3 proteins induced by lysine-4 or -9 mono-, di-, and tri-methylation, which are part of the critical post-translational modifications for DNA damage responses, using synchrotron radiation circular dichroism spectroscopy. The structural alterations occurred at residues not only close to but also distant from the methylated sites. These alterations would regulate the interactions between modified histones and other molecules and play an important role in DNA damage responses.

Academic Significance and Societal Importance of the Research Achievements

DNA損傷修復機構を理解する上で重要な要因の一つである修飾されたヒストンタンパク質の構造は未解明の部分が多い。本研究は、メチル化によりヒストンタンパク質H3全体の構造が変化し得ることを初めて示した。修飾によって生じるヒストンの構造変化過程を解明することは、DNA損傷修復機構の全容解明に寄与すると期待され、本研究はその端緒を開いたと考えられる。またこれらの成果は、将来的に、ヒストンの修飾を人工的に制御することで抗がん作用などの薬効を得るエピジェネティック薬の開発にもつながると期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (24 results)

All 2020 2019 2018 2017 Other

All Journal Article (5 results) (of which Peer Reviewed: 5 results,  Open Access: 3 results) Presentation (16 results) (of which Int'l Joint Research: 6 results,  Invited: 6 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Structural Alterations of Histone Proteins in DNA-Damaged Cells Revealed by Synchrotron Radiation Circular Dichroism Spectroscopy: A New Piece of the DNA-Damage-Response Puzzle2019

    • Author(s)
      Izumi Yudai
    • Journal Title

      Quantum Beam Science

      Volume: 3 Issue: 4 Pages: 23-23

    • DOI

      10.3390/qubs3040023

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Sample Volume Reduction Using the Schwarzschild Objective for a Circular Dichroism Spectrophotometer and an Application to the Structural Analysis of Lysine-36 Trimethylated Histone H3 Protein2018

    • Author(s)
      Izumi Yudai、Matsuo Koichi
    • Journal Title

      Molecules

      Volume: 23 Issue: 11 Pages: 2865-2865

    • DOI

      10.3390/molecules23112865

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Structural analysis of DNA repair protein XRCC4 applying circular dichroism in an aqueous solution.2018

    • Author(s)
      Nishikubo K, Izumi Y, Matsumoto Y, Fujii K, Matsuo K, Yokoya A.
    • Journal Title

      Radiat. Protect. Dosim.

      Volume: - Issue: 1-2 Pages: 1-4

    • DOI

      10.1093/rpd/ncy275

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Structural analysis of lysine-4 methylated histone H3 proteins using synchrotron radiation circular dichroism spectroscopy2018

    • Author(s)
      Izumi Yudai、Matsuo Koichi、Namatame Hirofumi
    • Journal Title

      Chirality

      Volume: 30 Issue: 5 Pages: 536-540

    • DOI

      10.1002/chir.22849

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Circular dichroism spectroscopic study on structural alterations of histones induced by post-translational modifications in DNA damage responses: Lysine-9 methylation of H32017

    • Author(s)
      Yudai Izumi, Koichi Mastuo, Kentaro Fujii, Akinari Yokoya, Masaki Taniguchi, Hirofumi Namatame
    • Journal Title

      Journal of Radiation Research

      Volume: 59 Issue: 2 Pages: 108-115

    • DOI

      10.1093/jrr/rrx068

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] 放射光円二色性分光によるK4メチル化ヒストンH3の構造解析2020

    • Author(s)
      泉 雄大、松尾 光一
    • Organizer
      第33回日本放射光学会年会 放射光科学合同シンポジウム
    • Related Report
      2019 Annual Research Report
  • [Presentation] 円偏光放射光を利用した量子生命科学研究2019

    • Author(s)
      泉 雄大
    • Organizer
      量子生命科学セミナー
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Structural changes of histone protein in DNA-damaged cells revealed by synchrotron radiation circular dichroism spectroscopy2019

    • Author(s)
      Yudai Izumi
    • Organizer
      The 2019 Korean-Japanese Student Workshop Pusan National University & Hiroshima University
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Secondary structure analysis of histone H2A-H2B proteins extracted from heated cells using circular dichroism spectroscopy2019

    • Author(s)
      Yudai Izumi and Koichi Matsuo
    • Organizer
      3rd QST International Symposium “Quantum Life Science”
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] エピジェネティクス研究への放射光・量子ビームの利用2019

    • Author(s)
      泉 雄大
    • Organizer
      第25回HiSOR研究会 小型放射光リングによる多彩な量子ビームの発生と応用
    • Related Report
      2019 Annual Research Report
  • [Presentation] 放射光円二色性分光によるK4/K9メチル化ヒストンH3の構造解析2019

    • Author(s)
      泉 雄大、松尾 光一
    • Organizer
      第13回日本エピジェネティクス研究会年会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Synchrotron radiation circular dichroism spectroscopy for structural characterization of chiral materials2018

    • Author(s)
      Yudai Izumi
    • Organizer
      MIRAI Materials Science Workshop 2018 Spring
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] DNA損傷に誘起されるヒストン構造変化の円二色性分光による解析2018

    • Author(s)
      泉 雄大
    • Organizer
      研究会「光・物質・生命・宇宙におけるキラリティ」
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 円二色性分光によるメチル化ヒストンH3の構造解析2018

    • Author(s)
      泉 雄大、松尾 光一
    • Organizer
      量子生命科学研究会第2回学術集会
    • Related Report
      2018 Research-status Report
  • [Presentation] 放射光円二色性分光を用いたK4メチル化ヒストンH3の構造解析2018

    • Author(s)
      泉 雄大、松尾 光一
    • Organizer
      第61回放射線化学討論会
    • Related Report
      2018 Research-status Report
  • [Presentation] Structural Analysis of Lysine-4 Methylated Histone H3 Using VUV-CD Spectroscopy2018

    • Author(s)
      Y. Izumi and K. Matsuo
    • Organizer
      The 22nd Hiroshima International Symposium on Synchrotron Radiation
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 放射光円二色性分光による メチル化ヒストンH3の構造解析2018

    • Author(s)
      泉 雄大、松尾 光一、藤井 健太郎、横谷 明徳
    • Organizer
      第31回日本放射光学会年会 放射光科学合同シンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] 放射光円二色性分光によるメチル化ヒストンH3の構造解析2017

    • Author(s)
      泉 雄大
    • Organizer
      第60回放射線化学討論会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 放射線生物学・量子生命科学研究における放射光円二色性分光の展開と可能性―ヒストン構造変化の観測を事例として―2017

    • Author(s)
      泉 雄大
    • Organizer
      日本放射線影響学会第60回大会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Structural analysis of lysine-4 methylated histone H3 proteins using synchrotron radiation circular dichroism spectroscopy2017

    • Author(s)
      Y. Izumi and K. Matsuo
    • Organizer
      Chirality 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Methylation-induced structural alteration of histone H3 characterized by synchrotron radiation circular dichroism spectroscopy2017

    • Author(s)
      Y. Izumi and K. Matsuo
    • Organizer
      1st QST International Symposium “Quantum Life Science”
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Book] Secondary structures of histone H3 proteins with unmethylated and methylated lysine-4 and -9 residues: Characterization using circular dichroism spectroscopy, In S. Jurga and J. Barciszewski (Eds.), The DNA, RNA, and Histone Methylomes2019

    • Author(s)
      Yudai Izumi
    • Total Pages
      16
    • Publisher
      Springer
    • Related Report
      2019 Annual Research Report
  • [Remarks] 広島大学 研究者総覧

    • URL

      http://seeds.office.hiroshima-u.ac.jp/profile/ja.138c038a163e3911520e17560c007669.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] メチル化によるヒストンタンパク質構造変化の初観測

    • URL

      http://www.hsrc.hiroshima-u.ac.jp/research/result/42.html

    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

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