| Project/Area Number |
17K13266
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Chemical biology
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | セレンテラジン / 生物発光 / リン / 硫黄 / 環化反応 / 分子イメージング / ピラジン |
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| Outline of Final Research Achievements |
We have studied on new synthetic methods of coelenterazine analogues bridged with a phosphorus or a sulfur in order to develop a near-infrared bioluminescence system. As the results, we found that sequential treatment of phosphonodithioic acid dithioesters with two Grignard reagents enabled the stepwise introduction of different carbon substituents on the phosphorus atom. We also found that treatment of phosphinic acid thioesters with benzyl Grignard reagents resulted in a substitution reaction at the sulfur atom to give a variety of benzyl sulfides.
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| Academic Significance and Societal Importance of the Research Achievements |
生きた生物の中を可視化するための技術を開発するべく、セレンテラジンと呼ばれる発光基質に対して、リンや硫黄などの第三周期元素を架橋部位として導入することを計画した。本研究では、リンや硫黄を炭素に導入する新しい手法の開発に成功し、この手法を利用することで、リンや硫黄で架橋された化合物群を合成できることを明らかにした。
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