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Construction of Carbon and Sulfur isotopic signatures in chemotrophic and phototrophic microbial ecosystem

Research Project

Project/Area Number 17K14412
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Stratigraphy/Paleontology
Research InstitutionTokyo Institute of Technology

Principal Investigator

Nakagawa Mayuko  東京工業大学, 地球生命研究所, 特任助教 (20647664)

Project Period (FY) 2017-04-01 – 2021-03-31
Project Status Completed (Fiscal Year 2020)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Keywords炭素同位体 / 硫黄同位体 / 微生物 / 代謝 / 炭素同位体比 / 硫黄同位体比 / 炭素循環 / 硫黄循環 / 微生物代謝 / 培養実験 / 環境試料 / 成層湖 / 温泉 / 湖 / 光合成細菌 / 安定同位体 / 古生態
Outline of Final Research Achievements

In this study, pure cultures of chemosynthetic and photosynthetic bacteria were conducted and analyzed the carbon and hydrogen isotope ratios of fatty acids. For sulfur isotope ratios, sulfate reduction and sulfur disproportionation were focused and characterized their quadruple sulfur isotopic signatures.
As for field studies, I was involved in carbon cycle analysis by analyzing hot spring water and sediments in Costa Rica, microbial community and carbon cycle analysis of stratified lake Waku-ike, and climate change analysis by analyzing sulfur isotopes in permafrost lake sediment samples in Mongolia.

Academic Significance and Societal Importance of the Research Achievements

化学合成・光合成細菌バイオマス中脂肪酸の炭素・水素同位体比はそれぞれ炭素固定回路とエネルギー代謝を反映した分別範囲を示した。また、異なる環境下における硫黄代謝の硫黄同位体分別係数を明らかにした。それらの純粋培養結果で得られた分別範囲は堆積物試料中の炭化水素化合物や硫黄化合物がどのような微生物代謝で供給されたかを解析するために重要な基礎データとなる。
実際にフィールド調査にて堆積物の炭素・硫黄同位体比解析により、新たに微生物の寄与を含んだ炭素循環モデルの提案や永久凍土が気候変化によって融解した影響による硫黄同位体的特徴を明らかにした。

Report

(5 results)
  • 2020 Annual Research Report   Final Research Report ( PDF )
  • 2019 Research-status Report
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (33 results)

All 2021 2020 2019 2018 2017 Other

All Int'l Joint Research (7 results) Journal Article (10 results) (of which Int'l Joint Research: 10 results,  Peer Reviewed: 10 results,  Open Access: 3 results) Presentation (16 results) (of which Int'l Joint Research: 9 results,  Invited: 3 results)

  • [Int'l Joint Research] Deep Carbon Observatry/Harvard University/Rutgers University(米国)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] University of Naples Federico II(イタリア)

    • Related Report
      2020 Annual Research Report
  • [Int'l Joint Research] Deep Carbon Observatry/Harvard University/Rutgers University(米国)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] University of Naples Federico II(イタリア)

    • Related Report
      2019 Research-status Report
  • [Int'l Joint Research] Deep Carbon Observatry/Harvard University/Rutgers University(米国)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] University of Naples Federico II(イタリア)

    • Related Report
      2018 Research-status Report
  • [Int'l Joint Research] ラトガース大学/カリフォルニア工科大学(米国)

    • Related Report
      2017 Research-status Report
  • [Journal Article] Effect of tectonic processes on biosphere-geosphere feedbacks across a convergent margin2021

    • Author(s)
      Fullerton Katherine M.、Nakagawa Mayuko、Giovannelli Donato、Lloyd Karen G. et al.
    • Journal Title

      Nature Geoscience

      Volume: 14 Issue: 5 Pages: 301-306

    • DOI

      10.1038/s41561-021-00725-0

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Changes in ATP Sulfurylase Activity in Response to Altered Cyanobacteria Growth Conditions2021

    • Author(s)
      Lucia Gastoldi, Lewis M Ward, Mayuko Nakagawa, Mario Giordano, Shawn E McGlynn
    • Journal Title

      Microbes and Environments

      Volume: 36 Issue: 2 Pages: n/a

    • DOI

      10.1264/jsme2.ME20145

    • NAID

      130008042862

    • ISSN
      1342-6311, 1347-4405
    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Sedimentary rhythm of Mn-carbonate laminae induced by East Asian summer monsoon variability and human activity in Lake Ohnuma, southwest Hokkaido, northern Japan2020

    • Author(s)
      Katsuta Nagayoshi、Naito Sayuri、Ikeda Hisashi、Tanaka Kuniaki、Murakami Takuma、Ochiai Shinya、Miyata Yoshiki、Shimizu Mayuko、Hayano Asuka、Fukui Konami、Hasegawa Hitoshi、Nagao Seiya、Nakagawa Mayuko、Nagashima Kana、Niwa Masakazu、Murayama Masafumi、Kagawa Masako、Kawakami Shin-ichi
    • Journal Title

      Quaternary Science Reviews

      Volume: 248 Pages: 106576-106576

    • DOI

      10.1016/j.quascirev.2020.106576

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] First investigation and absolute calibration of clumped isotopes in N 2 O by mid‐infrared laser spectroscopy2020

    • Author(s)
      Kantnerova Kristyna、Yu Longfei、Zindel Daniel、Zahniser Mark S.、Nelson David D.、Tuzson B?la、Nakagawa Mayuko、Toyoda Sakae、Yoshida Naohiro、Emmenegger Lukas、Bernasconi Stefano M.、Mohn Joachim
    • Journal Title

      Rapid Communications in Mass Spectrometry

      Volume: 34 Issue: 15

    • DOI

      10.1002/rcm.8836

    • Related Report
      2020 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A fluorination method for measuring 13C-13C isotopologue of C2 molecules2020

    • Author(s)
      Taguchi, K., Yamamoto, T., Nakagawa, M., Gilbert, A., Ueno, Y.,
    • Journal Title

      Rapid Communications in Mass Spectropetry

      Volume: 34 Issue: 11 Pages: 8761-8761

    • DOI

      10.1002/rcm.8761

    • Related Report
      2020 Annual Research Report 2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Siberian Permafrost Thawing Accelerated at the B?lling/Aller?d and Preboreal Warm Periods During the Last Deglaciation2019

    • Author(s)
      Katsuta Nagayoshi、Matsumoto Genki I.、Hase Yoshitaka、Tayasu Ichiro、Haraguchi Takashi F.、Tani Eriko、Shichi Koji、Murakami Takuma、Naito Sayuri、Nakagawa Mayuko、Hasegawa Hitoshi、Kawakami Shin‐ichi
    • Journal Title

      Geophysical Research Letters

      Volume: 46 Issue: 23 Pages: 13961-13971

    • DOI

      10.1029/2019gl084726

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Helium, inorganic and organic carbon isotopes of fluids and gases across the Costa Rica convergent margin2019

    • Author(s)
      Barry Peter H.、Nakagawa Mayuko、Giovannelli Donato、Maarten de Moor J.、Schrenk Matthew、Seltzer Alan M.、Manini Elena、Fattorini Daniele、di Carlo Marta、Regoli Francesco、Fullerton Katherine、Lloyd Karen G.
    • Journal Title

      Scientific Data

      Volume: 6 Issue: 1 Pages: 2052-4463

    • DOI

      10.1038/s41597-019-0302-4

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Hidden Concepts in the History and Philosophy of Origins-of-Life Studies: a Workshop Report.2019

    • Author(s)
      Mariscal, C., Barahona, A., Aubert-Kato, N. Nakagawa, M., et al.
    • Journal Title

      Origins of Life and Evolution of Biospheres

      Volume: 49 Issue: 3 Pages: 111-145

    • DOI

      10.1007/s11084-019-09580-x

    • Related Report
      2019 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Forearc carbon sink reduces long-term volatile recycling into the mantle2019

    • Author(s)
      PH Barry et al. (共著者37名中25番目)
    • Journal Title

      Nature

      Volume: 568 Issue: 7753 Pages: 487-492

    • DOI

      10.1038/s41586-019-1131-5

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Effect of light wavelength on hot spring microbial mat biodiversity2018

    • Author(s)
      Nishida Akifumi、Thiel Vera、Nakagawa Mayuko、Ayukawa Shotaro、Yamamura Masayuki
    • Journal Title

      PLOS ONE

      Volume: 13 Issue: 1 Pages: e0191650-e0191650

    • DOI

      10.1371/journal.pone.0191650

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 好熱性硫黄不均化菌の四種硫黄同位体指標2020

    • Author(s)
      中川 麻悠子, 梅澤 和寛, 小島 久弥, 福井 学, 吉田 尚弘, 上野 雄一郎
    • Organizer
      2020年度日本地球化学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] SIトレーサブルな安定炭素同位体比標準ガスの開発2020

    • Author(s)
      石川 真帆、下坂 琢哉、豊田 栄、山田 桂太、中川 麻悠子、Zhang Naizhong、吉田 尚弘
    • Organizer
      2020年度日本地球化学会
    • Related Report
      2020 Annual Research Report
  • [Presentation] 安定同位体で探る分子・生物・生態系の起源2020

    • Author(s)
      中川麻悠子
    • Organizer
      Kavli IPMU/ELSI/IRCN 第5回合同一般講演会
    • Related Report
      2020 Annual Research Report
    • Invited
  • [Presentation] Abandance of 13C-13C bonding in biotic and abiotic ethane measured by a new C2F6 method2020

    • Author(s)
      Koudai Taguchi, Tomonari Yamamoto, Mayuko Nakagaw, Alexis Gilbert, Yuichiro Ueno
    • Organizer
      Goldschmidt2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Iron-Rich Hot Springs in Japan, a Microbiogeochemical Guided Tour2020

    • Author(s)
      Li-Hau F, Nakagawa M, Ward L, Ueno Y & McGlynn S
    • Organizer
      Goldschmidt2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Equilibrium and Kinetic Fractionation on S-O Clumping of Sulfate2020

    • Author(s)
      Ueno Y, Nakagawa M, Katsuta T, Aoki R & Yoshida N
    • Organizer
      Goldschmidt2020
    • Related Report
      2020 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Helium and carbon isotopes in southern Costa Rica and western Panama2019

    • Author(s)
      Peter H Barry
    • Organizer
      AGU Fall Meeting 2019
    • Related Report
      2019 Research-status Report
    • Int'l Joint Research
  • [Presentation] 多次元アイソトポログ解析で分子の起源・環境を知る2019

    • Author(s)
      中川 麻悠子
    • Organizer
      第73回 イオン反応研究会 講演会
    • Related Report
      2019 Research-status Report
    • Invited
  • [Presentation] Carbon and hydrogen stable isotopic signatures of fatty acids produced by reductive tricarboxylic acid cycle2019

    • Author(s)
      M Nakagawa
    • Organizer
      Stable Isotopes in Biosphere Systems Workshops
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Chemical and biological carbon sinks in the Costa Rican Forearc: First insights from the Biology Meets Subduction project2018

    • Author(s)
      MJ de Moor, PH Barry, KG Lloyd, D Giovannelli, M Schrenk, M Nakagawa, CJ Ramirez, K Pratt, DR Hummer, TM Lopez
    • Organizer
      AGU Fall Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Multivariate and Network Analysis of Microbe-Environment Interactions Across a Geochemically Active Subduction Zone in Northern Costa Rica2018

    • Author(s)
      KM Fullerton, D Giovannelli, M Nakagawa, MO Schrenk, KG Lloyd
    • Organizer
      AGU Fall Meeting
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Carbon and Hydrogen stable isotopic signatures of fatty acids produced by reductive tricarboxylic acid cycle2018

    • Author(s)
      M Nakagawa, D Giovannelli, C Vetriani, M Kameya, A Gilbert, K Yamada, and N Yoshida
    • Organizer
      Extremophiles 2018
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 酸素濃度の異なる微生物生態系における脂肪酸 δC 及び δH 解析2018

    • Author(s)
      奥村 優 ,中川麻悠子,丹 佑太,豊田 栄 ,山田桂太 ,吉田尚弘
    • Organizer
      2018年度地球化学会年会
    • Related Report
      2018 Research-status Report
  • [Presentation] 安定同位体的指標を用いた生態系における一次生産の寄与評価2017

    • Author(s)
      中川麻悠子
    • Organizer
      光合成セミナー
    • Related Report
      2017 Research-status Report
  • [Presentation] 成層湖における季節及び深度毎の微生物相変化による炭素循環への影響2017

    • Author(s)
      中川麻悠子・大八木英夫・丹佑太・豊田栄・吉田尚弘
    • Organizer
      2017年度日本地球化学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] RELATIVE CONTRIBUTION ESTIMATIONS OF METABOLIC PROCESSES IN MICROBIAL ECOSYSTEMS WITH USING MULTIPLE ISOTOPIC ANALYSIS.2017

    • Author(s)
      Mayuko Nakagawa, Gilbert Alexis, Maxime Julien, Keita Yamada, Naohiro Yoshida
    • Organizer
      AbSciCon 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2017-04-28   Modified: 2023-01-30  

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