Development of next generation environmental sensing system using human iPS cells

• Project/Area Number: 17K14525
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Green/Environmental chemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: Tani Hidenori 国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (10635329)
• Project Period (FY): 2017-04-01 – 2020-03-31
• Project Status: Completed (Fiscal Year 2019)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: ヒトiPS細胞 / ノンコーディングRNA / 化学物質 / 環境センシング / バイオマーカー / 細胞・組織 / 生体分子

Outline of Final Research Achievements

In this study, we focused on two biological products as ideal tools for toxicological assessment: long non-coding RNAs (lncRNAs) and human-induced pluripotent stem cells (hiPSCs). lncRNAs are an important class of pervasive non-protein-coding transcripts involved in the molecular mechanisms associated with responses to cellular stresses. hiPSCs possess the capabilities of self-renewal and differentiation into multiple cell types, and they are free of the ethical issues associated with human embryonic stem cells. Here, we identified eight novel lncRNAs that respond to model chemical stresses in hiPSCs. Our results indicated that the lncRNAs responded to general and specific chemical stresses. We propose that these lncRNAs have the potential to be surrogate indicators of chemical stress responses in hiPSCs.

Academic Significance and Societal Importance of the Research Achievements

ヒトiPS細胞由来の分化細胞を用いた化学物質の生体影響評価において、新規生体分子である長鎖ノンコーティングRNAが毒性バイオマーカー、すなわち、サロゲート分子として有用であることを世界で初めて明らかにした。

Research Products

• [Journal Article] MicroRNA biomarkers for chemical hazard screening identified by RNA deep sequencing analysis in mouse embryonic stem cells (2020)
  • Author(s): Aoki Hiroshi、Tani Hidenori、Nakamura Kaoru、Sato Hiroaki、Torimura Masaki、Nakazato Tetsuya
  • Journal Title: Toxicology and Applied Pharmacology Volume: 392 Pages: 114929-114929
  • DOI: 10.1016/j.taap.2020.114929
  • Peer Reviewed / Open Access
• [Journal Article] Identification of RNA biomarkers for chemical safety screening in neural cells derived from mouse embryonic stem cells using RNA deep sequencing analysis. (2019)
  • Author(s): ani H, Matsutani T, Aoki H, Nakamura K, Hamaguchi Y, Nakazato T, Hamada M.
  • Journal Title: Biochem Biophys Res Commun. Volume: 512 Issue: 4 Pages: 641-646
  • DOI: 10.1016/j.bbrc.2018.11.141
  • Peer Reviewed / Open Access
• [Journal Article] Short-lived long noncoding RNAs as surrogate indicators for chemical stress in HepG2 cells and their degradation by nuclear RNases (2019)
  • Author(s): Tani Hidenori、Numajiri Ayaka、Aoki Motohide、Umemura Tomonari、Nakazato Tetsuya
  • Journal Title: Scientific Reports Volume: 9 Issue: 1 Pages: 20299-20299
  • DOI: 10.1038/s41598-019-56869-y
  • Peer Reviewed / Open Access
• [Journal Article] Multiplicity in long noncoding RNA in living cells (2018)
  • Author(s): Kurokara R, Komiya R, Oyoshi T, Matsuno Y, Tani H, Katahira M, Hitachi K, Iwashita Y, Yamashita T, Kondo K, Yoneda R, Yamaoki Y, Ueda N, Mashima T, Kobayashi N, Nagata T, Kiyoishi A, Miyake M, Kano F, Murata M, Hamad N, Sasaki K, Shoji N.
  • Journal Title: Biomedical Sciences Volume: 4 Pages: 18-23
  • Peer Reviewed
• [Journal Article] ノンコーディングRNAから生命の謎を解き明かす (2018)
  • Author(s): 谷英典
  • Journal Title: PHARM TECH JAPAN Volume: 5 Pages: 18-19
  • Peer Reviewed
• [Journal Article] 蛍光色素及び修飾核酸を利用した生体分子解析技術の開発とその応用 (2018)
  • Author(s): 谷英典
  • Journal Title: ぶんせき Volume: 8 Pages: 342-342
  • NAID: 130007607524
  • Peer Reviewed
• [Journal Article] 蛍光色素及び修飾核酸を利用した生体分子解析技術の開発とその応用 (2018)
  • Author(s): 谷英典
  • Journal Title: 蛍光色素及び修飾核酸を利用した生体分子解析技術の開発とその応用” 分析化学 Volume: 2 Pages: 109-116
  • NAID: 130007607524
  • Peer Reviewed
• [Journal Article] Rapid monitoring of RNA degradation activity in vivo for mammalian cells. (2017)
  • Author(s): Tani H, Sato H, Torimura M.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 123 Issue: 4 Pages: 523-527
  • DOI: 10.1016/j.jbiosc.2016.11.010
  • NAID: 40021178856
  • Peer Reviewed / Open Access
• [Journal Article] Short-lived long non-coding RNAs as surrogate indicators for chemical exposure and LINC00152 and MALAT1 modulate their neighboring genes. (2017)
  • Author(s): Tani H, Okuda S, Nakamura K, Aoki M, Umemura T.
  • Journal Title: PLoS One Volume: 12 Issue: 7 Pages: e0181628-e0181628
  • DOI: 10.1371/journal.pone.0181628
  • Peer Reviewed / Open Access
• [Journal Article] Identification of RNA biomarkers for chemical safety screening in mouse embryonic stem cells using RNA deep sequencing analysis (2017)
  • Author(s): Hidenori Tani, Jun-ichi Takeshita, Hiroshi Aoki, Kaoru Nakamura, Ryosuke Abe, Akinobu Toyoda, Yasunori Endo, Sadaaki Miyamoto, Masashi Gamo, Hiroaki Sato, Masaki Torimura
  • Journal Title: PLOS ONE Volume: 12 Issue: 7 Pages: e0182032-e0182032
  • DOI: 10.1371/journal.pone.0182032
  • NAID: 120007128838
  • Peer Reviewed / Open Access
• [Journal Article] Effect of methyl p-hydroxybenzoate on the culture of mammalian cell (2017)
  • Author(s): Hidenori Tani, Jun-ichi Takeshita, Hiroshi Aoki, Kaoru Nakamura, Ryosuke Abe, Akinobu Toyoda, Yasunori Endo, Sadaaki Miyamoto, Masashi Gamo, Hiroaki Sato, Masaki Torimura
  • Journal Title: Drug Discoveries & Therapeutics Volume: 11 Issue: 5 Pages: 276-280
  • DOI: 10.5582/ddt.2017.01054
  • NAID: 130006210844
  • ISSN: 1881-7831, 1881-784X
  • Peer Reviewed / Open Access
• [Presentation] 蛍光色素及び修飾核酸を利用した生体分子解析技術の開発とその応用 (2018)
  • Author(s): 谷 英典
  • Organizer: 第67回日本分析化学会年会
  • Invited
• [Presentation] 長鎖ノンコーディングRNAに関するRNA分解からのアプローチ (2018)
  • Author(s): 谷 英典
  • Organizer: 第2回長鎖非コードRNA勉強会
  • Invited
• [Presentation] ノンコーディングRNAに着目した化学物質の生体影響評価技術の開発 (2017)
  • Author(s): 谷 英典
  • Organizer: 京都大学iPS細胞研究所セミナー
  • Invited
• [Presentation] 長鎖ノンコーディングRNAに関するRNA分解からのアプローチ (2017)
  • Author(s): 谷 英典
  • Organizer: 第1回長鎖非コードRNA勉強会
  • Invited
• [Presentation] BRIC-seq: Genome-wide analysis of RNA turnover. (2017)
  • Author(s): Tani H.
  • Organizer: Eukaryotic RNA turnover
  • Int'l Joint Research
• [Presentation] LINC00152及びMALAT1は近傍の遺伝子発現を制御する (2017)
  • Author(s): 谷 英典、奥田 彩也夏、中村 薫、青木 元秀、梅村 知也
  • Organizer: 第19回 日本RNA学会年会
• [Remarks] Hidenori TANI's Homepage
  • URL: https://staff.aist.go.jp/h.tani/