Development of next generation environmental sensing system using human iPS cells
Project/Area Number |
17K14525
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Green/Environmental chemistry
|
Research Institution | National Institute of Advanced Industrial Science and Technology |
Principal Investigator |
Tani Hidenori 国立研究開発法人産業技術総合研究所, エネルギー・環境領域, 主任研究員 (10635329)
|
Project Period (FY) |
2017-04-01 – 2020-03-31
|
Project Status |
Completed (Fiscal Year 2019)
|
Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
Keywords | ヒトiPS細胞 / ノンコーディングRNA / 化学物質 / 環境センシング / バイオマーカー / 細胞・組織 / 生体分子 |
Outline of Final Research Achievements |
In this study, we focused on two biological products as ideal tools for toxicological assessment: long non-coding RNAs (lncRNAs) and human-induced pluripotent stem cells (hiPSCs). lncRNAs are an important class of pervasive non-protein-coding transcripts involved in the molecular mechanisms associated with responses to cellular stresses. hiPSCs possess the capabilities of self-renewal and differentiation into multiple cell types, and they are free of the ethical issues associated with human embryonic stem cells. Here, we identified eight novel lncRNAs that respond to model chemical stresses in hiPSCs. Our results indicated that the lncRNAs responded to general and specific chemical stresses. We propose that these lncRNAs have the potential to be surrogate indicators of chemical stress responses in hiPSCs.
|
Academic Significance and Societal Importance of the Research Achievements |
ヒトiPS細胞由来の分化細胞を用いた化学物質の生体影響評価において、新規生体分子である長鎖ノンコーティングRNAが毒性バイオマーカー、すなわち、サロゲート分子として有用であることを世界で初めて明らかにした。
|
Report
(4 results)
Research Products
(18 results)
-
-
-
-
[Journal Article] Multiplicity in long noncoding RNA in living cells2018
Author(s)
Kurokara R, Komiya R, Oyoshi T, Matsuno Y, Tani H, Katahira M, Hitachi K, Iwashita Y, Yamashita T, Kondo K, Yoneda R, Yamaoki Y, Ueda N, Mashima T, Kobayashi N, Nagata T, Kiyoishi A, Miyake M, Kano F, Murata M, Hamad N, Sasaki K, Shoji N.
-
Journal Title
Biomedical Sciences
Volume: 4
Pages: 18-23
Related Report
Peer Reviewed
-
-
-
-
-
-
[Journal Article] Identification of RNA biomarkers for chemical safety screening in mouse embryonic stem cells using RNA deep sequencing analysis2017
Author(s)
Hidenori Tani, Jun-ichi Takeshita, Hiroshi Aoki, Kaoru Nakamura, Ryosuke Abe, Akinobu Toyoda, Yasunori Endo, Sadaaki Miyamoto, Masashi Gamo, Hiroaki Sato, Masaki Torimura
-
Journal Title
PLOS ONE
Volume: 12
Issue: 7
Pages: e0182032-e0182032
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
[Journal Article] Effect of methyl p-hydroxybenzoate on the culture of mammalian cell2017
Author(s)
Hidenori Tani, Jun-ichi Takeshita, Hiroshi Aoki, Kaoru Nakamura, Ryosuke Abe, Akinobu Toyoda, Yasunori Endo, Sadaaki Miyamoto, Masashi Gamo, Hiroaki Sato, Masaki Torimura
-
Journal Title
Drug Discoveries & Therapeutics
Volume: 11
Issue: 5
Pages: 276-280
DOI
NAID
ISSN
1881-7831, 1881-784X
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-