• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Development of an innovative oocyte collection system in cynomolgus monkey

Research Project

Project/Area Number 17K14977
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Laboratory animal science
Research InstitutionShiga University of Medical Science

Principal Investigator

Seita Yasunari  滋賀医科大学, 動物生命科学研究センター, 特任講師 (80737023)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords過剰排卵 / カニクイザル / 発生工学 / FSH
Outline of Final Research Achievements

Cynomolgus monkeys is considered to be a valuable model organism for human disease modeling. It has been reported by many laboratories that mature oocytes can be recovered from cynomolgus monkey through the ovarian stimulation by human follicle-stimulating hormone (hFSH), yet it is not known whether mature oocytes can be efficiently obtained through second ovarian stimulation by hFSH. We report that some of the ovaries were stimulated efficiently by hFSH even after first ovum pick up, but the others were stimulated poorly by hFSH. The antibodies against hFSH were detected in the serum of female monkeys with the poorly stimulated ovaries. Collectively, these data suggest that production of anti-hFSH antibodies in the serum may cause the poor ovarian response to hFSH stimulation, and the detection of the antibody as well as the observation of the ovary over the course of hFSH administration is useful to predict the favorable second ovarian stimulation by human FSH.

Academic Significance and Societal Importance of the Research Achievements

カニクイザルはヒトにもっとも近い使用可能な実験動物である。ゲノム編集技術の発達から今後多くのヒト疾患モデルがカニクイザルを用いて作出されることが予想され、ゲノム編集に使用される卵の安定的な供給は非常に重要である。本研究では複数回採卵時に採卵数が減少する一因(FSH抗体の産生)を明らかにした事から今後FSH抗体の産生を抑制する採卵方法を開発すれば、卵の安定供給に繋がり、ヒト疾患モデルの効率的な作製に繋がることが予想される。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (5 results)

All 2019 2018 2017

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Poor second ovarian stimulation in cynomolgus monkeys <i>(Macaca fascicularis)</i> is associated with the production of antibodies against human follicle-stimulating hormone2019

    • Author(s)
      SEITA Yasunari、IWATANI Chizuru、TSUCHIYA Hideaki、NAKAMURA Shinichiro、KIMURA Fuminori、MURAKAMI Takashi、EMA Masatsugu
    • Journal Title

      Journal of Reproduction and Development

      Volume: 65 Issue: 3 Pages: 267-273

    • DOI

      10.1262/jrd.2018-156

    • NAID

      130007663050

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Comprehensive evaluation of ubiquitous promoters suitable for the generation of transgenic cynomolgus monkeys†2019

    • Author(s)
      Seita Yasunari、Tsukiyama Tomoyuki、Azami Takuya、Kobayashi Kenichi、Iwatani Chizuru、Tsuchiya Hideaki、Nakaya Masataka、Tanabe Hideyuki、Hitoshi Seiji、Miyoshi Hiroyuki、Nakamura Shinichiro、Kawauchi Akihiro、Ema Masatsugu
    • Journal Title

      Biology of Reproduction

      Volume: 印刷中 Issue: 6 Pages: 1440-1452

    • DOI

      10.1093/biolre/ioz040

    • NAID

      120006594188

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] トランスジェニックカニクイザル作製のためのユビキタスプロモーターの検討2018

    • Author(s)
      清田 弥寿成、築山 智之、浅見 拓哉、岩谷 千鶴、土屋 英明、中村 紳一郎、依馬 正次
    • Organizer
      第111会日本繁殖生物学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Anti-FSH antibodies associate with poor response of ovarian stimulation in IVF of cynomolgus monkey.2017

    • Author(s)
      Yasunari Seita, Jun Matsushita, Chizuru Iwatani, Hideaki Tsuchiya, Masatsugu Ema
    • Organizer
      50th Annual Meeting of the Society for the Study of Reproduction
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Reduced ovarian response after 2nd human FSH administration is caused by production of antibody against human FSH in cynomolgus monkey.2017

    • Author(s)
      Yasunari Seita, Jun Matsushita, Chizuru Iwatani, Hideaki Tsuchiya, Masatsugu Ema
    • Organizer
      The Forth World Congress for Reproductive Biology
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

URL: 

Published: 2017-04-28   Modified: 2020-03-30  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi