Mechanisms for phase separation of RNA-induced nuclear bodies
| Project/Area Number |
17K15058
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Molecular biology
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | 長鎖ノンコーディングRNA（lncRNA） / NEAT1 / 相分離 / 核内構造体 / 天然変性領域 / RNA結合タンパク質 / ヒト一倍体細胞 / CRISPR/Cas9 / Long noncoding RNA / 相転移 / パラスペックル / Low complexity domain / ヒト一倍体細胞株 / lncRNA / arcRNA |
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| Outline of Final Research Achievements |
Among long noncoding RNAs (lncRNAs) transcribed from vast majority of mammalian genomes, a class of them plays an essential role in construction of nuclear bodies. We analyzed paraspeckle nuclear body constructed by NEAT1 lncRNA as a model to investigate how the lncRNAs dictate their functions. We identified that several NEAT1 RNA domains determine NEAT1 stability, isoform switching, and paraspeckle formation through phase separation. Especially, the middle domain of NEAT1 has an essential role in the formation of phase-separated paraspeckle by interacting with partner RNA-binding proteins.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究により、癌関連ノンコーディングRNAであるNEAT1が核内構造体パラスペックルを作り上げるために必要なRNA領域の同定に成功した。具体的には、複数の機能ドメインが固有の機能を発揮することで構造体が作られることが明らかとなった。本研究成果は、癌を始めとする種々の疾患との関連が明らかになっているNEAT1の疾患における機能を詳細に理解する上で重要である。さらに今後、ノンコーディングRNA機能の体系的な理解に大きく寄与することや応用技術の開発などに繋がることが期待できる。
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Report
(3 results)
Research Products
(29 results)
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[Presentation] NEAT1 modular lncRNA domains dictate formation of phase-separated, distinct, highly ordered paraspeckle2019
Author(s)
Tomohiro Yamazaki, Sylvie Souquere, Hyura Yoshino, Hiro Takakuwa, Takeshi Chujo, Archa H. Fox, Charles S. Bond, Shinichi Nakagawa, Gerard Pierron, Tetsuro Hirose
Organizer
The 20th Takeda Science Foundation Symposium on Bioscience: RNA Neobiology
Related Report
Int'l Joint Research
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[Presentation] Multiple NEAT1 modular RNA domains cooperatively construct phase-separated, distinct, highly ordered paraspeckle nuclear bodies2018
Author(s)
Tomohiro Yamazaki, Souquere Sylvie, Hiro Takakuwa, Hyura Yoshino, Takeshi Chujo, Archa H. Fox, Charles S. Bond, Shinichi Nakagawa, Gerard Pierron, Tetsuro Hirose
Organizer
JAJ RNA 2018
Related Report
Int'l Joint Research
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[Book] Applied RNA Bioscience2018
Author(s)
Seiji Masuda, Shingo Izawa, Sae Kato, Kei Kitahara, Takahiro Yamauchi, Naofumi Ito, Yuji Morita, Atsushi Ogawa, Kei Endo, Naoyuki Kataoka, Intisar Fouad Ali Mursi, Takayoshi Imai, Tomohiro Yamazaki, Eleonora Leucci, Yoshiya Ikawa, Jennifer Chu, Songling Bai, Yuhya Wakasa
Total Pages
286
Publisher
Springer
ISBN
9789811083716
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