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Structure based analysis of human herpesvirus 6 transactivator IE2

Research Project

Project/Area Number 17K15075
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Structural biochemistry
Research InstitutionKobe University

Principal Investigator

Nishimura Mitsuhiro  神戸大学, 医学研究科, 助教 (40510285)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsヒトヘルペスウイルス6 / X線結晶構造解析 / X線結晶解析 / ウイルス / 蛋白質 / X線構造解析 / 転写活性化因子
Outline of Final Research Achievements

研究成果の概要(英文):
Human herpesvirus 6 infects almost all humans during infancy, although the infection mechanism and pathogenicity still remain unclear. A HHV-6 viral protein, immediate early protein 2 (IE2), expresses immediately after the infection and promote the expression of the other viral factors as a transactivator. In this study, the structure and function of IE2 protein were analyzed. A DNA binding-model of IE2 C-terminal domain (IE2-CTD) was constructed based on the determined structure. Putative functional sites of IE2-CTD were proposed from the structural analysis, and several sites were proved to be significant on the IE2 transactivation function by a mutation analysis.

Academic Significance and Societal Importance of the Research Achievements

本研究ではヒトヘルペスウイルス6の感染開始時に重要な働きを持つIE2についてそのC末端領域の立体構造を詳細に解析し、IE2の転写活性化機能に必要な機能部位を新たに見出すことができた。これらの成果はHHV-6の感染において、実際にIE2がウイルスタンパク質としてどのように機能するかをその立体構造に基づいて解析したものであり、さらに今後IE2がヒト細胞内の宿主因子とどのように相互作用するかを解析していくための手掛かりとなることで、ウイルス感染機構の解明及びその制御法開発へ繋がる事が期待できる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (6 results)

All 2017

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (4 results) (of which Int'l Joint Research: 2 results)

  • [Journal Article] Crystal Structure of the DNA-Binding Domain of Human Herpesvirus 6A Immediate Early Protein 22017

    • Author(s)
      Nishimura Mitsuhiro、Wang Junjie、Wakata Aika、Sakamoto Kento、Mori Yasuko
    • Journal Title

      Journal of Virology

      Volume: 91 Issue: 21

    • DOI

      10.1128/jvi.01121-17

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Purification, Crystallization and X-ray Diffraction Study of the Cterminal Domain of Human Herpesvirus 6A Immediate Early Protein 22017

    • Author(s)
      WANG JUNJIE, NISHIMURA MITSUHIRO, WAKATA AIKA、MORI YASUKO
    • Journal Title

      Kobe Journal Medical Sciences

      Volume: 62

    • NAID

      120006220839

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] ヒトヘルペスウイルス6A 転写活性化因子IE2 C末端領域の立体構造解析2017

    • Author(s)
      西村 光広、若田 愛加、王 俊杰、森 康子
    • Organizer
      第31回ヘルペスウイルス研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] Structure-based analysis of human herpesvirus 6A immediate early protein 22017

    • Author(s)
      Mitsuhiro Nishimura, Aika Wakata, Kento Sakamoto, Wang Junjie, Yasuko Mori
    • Organizer
      10th International Conference on HHV-6 & 7
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Structure of Human Herpesvirus 6A Immediate Early Protein 2 C-terminal Domain Revealed a Similarity with DNA Binding Proteins of Gammaherpesvirus2017

    • Author(s)
      Mitsuhiro Nishimura, Wang Junjie, Aika Wakata, Kento Sakamoto, Yasuko Mori
    • Organizer
      42nd Annual international herpesvirus workshop
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Structural analysis of human herpesvirus 6A immediate early protein 2 C-terminal Domain2017

    • Author(s)
      Mitsuhiro Nishimura, Aika Wakata, Junjie Wang, Yasuko Mori
    • Organizer
      第65回 日本ウイルス学会学術集会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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