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Development of real-time coding imaging method for minimally invasive in vivo nano measurement of the heart

Research Project

Project/Area Number 17K15102
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biophysics
Research InstitutionChubu University

Principal Investigator

SHINTANI Seine  中部大学, 生命健康科学部, 助教 (40650536)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2019: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Keywords電子顕微鏡ライブイメージング / コンピュテーショナルフォトグラフィ / 走査型電子顕微鏡 / 液体レンズ / 画像解析 / 生物物理学 / バイオイメージング / 低侵襲In vivoナノ計測 / 心臓イメージング / ロボット顕微鏡システム / 符号化撮像 / 電子顕微鏡 / In vivo imaging / 心臓拍動
Outline of Final Research Achievements

The method of analyzing the behavior of biomolecules and their aggregates inside cells is almost limited to live imaging with an optical microscope using an objective lens with a high numerical aperture. However, using a 100x oil immersion objective with a numerical aperture of 1.4, the depth of focus is less than 300 nanometers. In order to measure a three-dimensional and dynamic measurement target with high resolution, we have developed the following two measurement methods. 1. An optical microscope system that extends the depth of focus by performing deconvolution of the point spread function on the image obtained by moving the liquid lens whose curvature changes with the applied voltage at high speed. 2. A method of live imaging a biological sample in a solution environment with a scanning electron microscope. It is expected to be applied to microscopic live imaging of three-dimensional and dynamic measurement targets such as the heart.

Academic Significance and Societal Importance of the Research Achievements

従来困難であった、立体的で動的な計測対象の高分解能なライブイメージングを2種類の方法で可能にした。液体レンズを組み入れた光学顕微鏡系は、端的に言えば従来の光学顕微鏡系の高さ方向の計測領域を拡張するものである。もう一方の溶液環境下の生体試料を走査型電子顕微鏡でライブイメージングする方法は、高分解能計測と立体的で動的な対象の計測を両立させる方法として進歩性が大きい。具体的には、高さ方向の計測領域が光学顕微鏡系の50倍以上で直径20nmの繊維の計測も行えた。Abbeの公式に基づく光学顕微鏡の分解能は200nmである。その10倍以上の分解能をもつ本手法は様々な計測に活用できると期待される。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (35 results)

All 2020 2019 2018 2017 Other

All Journal Article (8 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 8 results,  Open Access: 8 results) Presentation (20 results) (of which Int'l Joint Research: 12 results,  Invited: 1 results) Remarks (7 results)

  • [Journal Article] Single-cell temperature mapping with fluorescent thermometer nanosheets2020

    • Author(s)
      Oyama Kotaro、Gotoh Mizuho、Hosaka Yuji、Oyama Tomoko G.、Kubonoya Aya、Suzuki Yuma、Arai Tomomi、Tsukamoto Seiichi、Kawamura Yuki、Itoh Hideki、Shintani Seine A.、Yamazawa Toshiko、Taguchi Mitsumasa、Ishiwata Shin’ichi、Fukuda Norio
    • Journal Title

      Journal of General Physiology

      Volume: 152 Issue: 8

    • DOI

      10.1085/jgp.201912469

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Thermal activation of thin filaments in striated muscle2020

    • Author(s)
      Shuya Ishii, Kotaro Oyama, Seine A. Shintani, Fuyu Kobirumaki-Shimozawa, Shin'Ichi Ishiwata, Norio Fukuda.
    • Journal Title

      Frontiers in Physiology, section Striated Muscle Physiology

      Volume: 11 Pages: 278-278

    • DOI

      10.3389/fphys.2020.00278

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Curriculum for Programming and Artificial Intelligence Education for Biomedical Science Students -From the Basics of the Python Language to Artificial Intelligence Algorithms for Generation-2020

    • Author(s)
      Shintani SA
    • Journal Title

      Bulletin of Institute of Life and Health Sciences, Chubu University

      Volume: 16 Pages: 40-49

    • NAID

      120006825340

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Tri-functional calcium-deficient calcium titanate coating on titanium metal by chemical and heat treatment2019

    • Author(s)
      Yamaguchi S, Le P, Ito M, Shintani SA, Takadama H
    • Journal Title

      Coatings

      Volume: 9 Issue: 9 Pages: 561-561

    • DOI

      10.3390/coatings9090561

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of myofibril passive elastic properties on the mechanical communication between mo-tor proteins on adjacent sarcomeres.2019

    • Author(s)
      9.Takumi Washio*, Seine A. Shintani, Hideo Higuchi, Seiryo Sugiura and Toshiaki Hisada.
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 1-11

    • DOI

      10.1038/s41598-019-45772-1

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Microscopic heat pulses activate cardiac thin filaments2019

    • Author(s)
      Ishii Shuya、Oyama Kotaro、Arai Tomomi、Itoh Hideki、Shintani Seine A.、Suzuki Madoka、Kobirumaki-Shimozawa Fuyu、Terui Takako、Fukuda Norio、Ishiwata Shin'ichi
    • Journal Title

      Journal of General Physiology

      Volume: 151 Issue: 6 Pages: 860-869

    • DOI

      10.1085/jgp.201812243

    • Related Report
      2019 Annual Research Report 2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Development of a general-purpose algorithm to measure motion and state of attention object - Study report on method of using deep learning for quantitative science research -2018

    • Author(s)
      Shintani SA
    • Journal Title

      Annual report of Research Institute of Life and Health Sciences

      Volume: 14 Pages: 45-49

    • NAID

      120006521087

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Dynamic properties of bio-motile systems as a liquid-crystalline structure2017

    • Author(s)
      Shin'ichi Ishiwata, Makito Miyazaki, Katsuhiko Sato, Koutaro Nakagome, Seine A. Shintani, Fuyu Kobirumaki-Shimozawa, Norio Fukuda, Kazuya Suzuki, Jun Takagi, Yuta Shimamoto, and Takeshi Itabashi
    • Journal Title

      Mol. Cryst. Liq. Cryst.

      Volume: 印刷中 Issue: 1 Pages: 127-150

    • DOI

      10.1080/15421406.2017.1289445

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Molecular mechanisms of contraction rhythm homeostasis in warmed cardiomyocytes.2020

    • Author(s)
      Seine A. Shintani, Shin’ichi Ishiwata, Norio Fukuda
    • Organizer
      The 97th Annual Meeting of the Physiological Society of Japan
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Formation of alumina layer on titanium alloy by micro-arc oxidation for bearing surface of artificial joint.2019

    • Author(s)
      Hiroaki Takadama, Rohit Khanna, Seine A. Shintani, and Seiji Yamaguchi
    • Organizer
      The 13th Pacific Rim Conference of Ceramic Societies
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Apatite formation and antibacterial activity of Ti and Ti-6Al-4V alloy incorporated with Ca, Sr and Ag ions.2019

    • Author(s)
      Rina Iwatsuki, Seiji Yamaguchi, Seine A. Shintani and Hiroaki Takadama
    • Organizer
      The 13th Pacific Rim Conference of Ceramic Societies
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elucidation of molecular mechanism of contraction rhythm homeostasis in cardiac myocytes. (Symposium)2019

    • Author(s)
      Seine A. Shintani, Takumi Washio
    • Organizer
      The 57th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Elucidation of molecular mechanism of contraction rhythm homeostasis in cardiac myocytes. (Poster)2019

    • Author(s)
      Seine A. Shintani, Takumi Washio
    • Organizer
      The 57th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 心臓のマルチスケールな構造に備わっている温度を活用する仕組みの研究2019

    • Author(s)
      新谷正嶺,樋口秀男,鷲尾巧
    • Organizer
      温熱生理研究会
    • Related Report
      2019 Annual Research Report
  • [Presentation] 心筋細胞に備わる収縮リズム恒常性の分子機構の解明2019

    • Author(s)
      新谷正嶺
    • Organizer
      第7回「知の創発」研究拠点プロジェクト研究会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] Rapid heating induces high-frequency sarcomeric oscillations in living rat neonatal cardiomyocytes2019

    • Author(s)
      Shintani SA., Oyama K., Ishiwata S. and Fukuda N.
    • Organizer
      9th FAOPS Congress
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Microscopic heat pulses induce activation of cardiac thin filaments in the in vitro motility assay2019

    • Author(s)
      Ishii S., Oyama K., Arai T., Itoh H., Shintani SA., Suzuki M., Kobirumaki-Shimozawa F., Terui T., Fukuda N. and Ishiwata S.
    • Organizer
      9th FAOPS Congress
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Mechanical wave characteristics generated by sarcomere group of cardiac / skeletal muscle2018

    • Author(s)
      Shintani SA.
    • Organizer
      The 56th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] A Effect of metal ions on type I collagen production of L929 fibroblasts.2018

    • Author(s)
      Yamaguchi S., Niwa Y., Ito M., Shintani SA. and Takadama H.
    • Organizer
      The 29th European Society for Biomaterials Annual Conference
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Iodine-loaded bioactive titanium metal and its alloys by chemical and heat treatment2018

    • Author(s)
      Yamaguchi S., Ito M., Shintani SA., Nakamura T. and Takadama H.
    • Organizer
      30th Symposium and Annual Meeting of the International Society for Ceramics in Medicine
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] Molecular mechanism of self-oscillatory contraction of cardiac muscle2018

    • Author(s)
      Shintani SA., Washio T., Hwang Y., Kaya M. and Higuchi H.
    • Organizer
      12th International Symposium on Nanomedicine
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] 温めた心筋細胞に備わる収縮リズム恒常性とその分子機構の考察2018

    • Author(s)
      新谷正嶺、鷲尾巧、樋口秀男
    • Organizer
      平成30年度 温熱生理研究会
    • Related Report
      2018 Research-status Report
  • [Presentation] 実験とシミュレーションの比較から考察する温めた心筋細胞に生じる収縮リズム恒常性の役割2018

    • Author(s)
      新谷正嶺、鷲尾巧、樋口秀男
    • Organizer
      2018年 生体運動研究合同班会議
    • Related Report
      2017 Research-status Report
  • [Presentation] 温めた心筋細胞に備わった収縮リズム恒常性2018

    • Author(s)
      新谷正嶺、鷲尾巧
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 光熱変換顕微鏡を用いた温度センシングの一細胞解析2018

    • Author(s)
      大山廣太郎,Zeeb Vadim、新井智美、伊藤秀城、新谷正嶺、鈴木団、福田紀男、石渡信一
    • Organizer
      第95回日本生理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Constant beating frequency of sarcomeres in cardiomyocytes regulated ingeniously by collective motion of myosin molecules2017

    • Author(s)
      Shintani SA, Washio T, Higuchi H
    • Organizer
      The 55th Annual Meeting of the Biophysical Society of Japan
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 心筋細胞に備わった昇温誘起の収縮振動はミオシンの巧みな集団運動で応答性を保ち振動周期を一定に調整する2017

    • Author(s)
      新谷正嶺、鷲尾巧、樋口秀男
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Research-status Report
  • [Presentation] サルコメア収縮の蛍光イメージングと光操作技術の開発2017

    • Author(s)
      大山廣太郎、新谷正嶺、塚本精一、小比類巻生、下澤東吾、鈴木団、石渡信一、福田紀男
    • Organizer
      ジストロフィン欠損モデル動物を基盤とした筋ジストロフィーの新しい治療法開発 平成2 9年度 研究班会議
    • Related Report
      2017 Research-status Report
  • [Remarks] researchmapの研究所情報ページ

    • URL

      https://researchmap.jp/Seine_A_Shintani

    • Related Report
      2019 Annual Research Report
  • [Remarks] 所属大学の教員情報ページ

    • URL

      https://www.chubu.ac.jp/about/faculty/profile/b44e9f346b010f4dcaa34e30b1ced0d3e3c67b9a.html

    • Related Report
      2019 Annual Research Report
  • [Remarks] 研究成果のプレスリリース

    • URL

      https://www3.chubu.ac.jp/research/news/25144/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 研究成果のプレスリリース

    • URL

      https://www3.chubu.ac.jp/research/news/24905/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 大学ジャーナルの研究成果の記事

    • URL

      https://univ-journal.jp/26703/

    • Related Report
      2019 Annual Research Report
  • [Remarks] 中部大学 教員情報 新谷正嶺

    • URL

      https://www.chubu.ac.jp/about/faculty/profile/b44e9f346b010f4dcaa34e30b1ced0d3e3c67b9a.html

    • Related Report
      2018 Research-status Report
  • [Remarks] researchmap 研究者情報 新谷正嶺

    • URL

      https://researchmap.jp/7000003401/

    • Related Report
      2018 Research-status Report

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Published: 2017-04-28   Modified: 2024-01-30  

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