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Elucidation of flagellar/ciliary assembly mechanism by tubulin polyglutamylation

Research Project

Project/Area Number 17K15115
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionUniversity of Yamanashi

Principal Investigator

KUBO Tomohiro  山梨大学, 大学院総合研究部, 特任助教 (70778745)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords鞭毛・繊毛 / クラミドモナス / チューブリン / 翻訳後修飾 / ポリグルタミン酸化 / TTLL / 運動性 / 鞭毛 / 繊毛 / チューブリン翻訳後修飾 / ポリグルタミン酸化修飾 / クライオ電子顕微鏡
Outline of Final Research Achievements

To investigate the function of tubulin polyglutamylation in flagellar assembly, I analyzed a novel Chlamydomonas mutant ssa11 lacking a protein homologous to mammalian polyglutamylase TTLL6. When combined with the previously investigated tpg1 mutant (lacking Chlamydomonas TTLL9), ssa11 exhibited a dramatic reduction of axonemal polyglutamylation even more than the tpg1, thereby causing a severe motility defect. Interestingly, ssa11tpg1 had slower flagellar regeneration kinetics compared to wild type. This result suggests that tubulin polyglutamylation affects the stability of axonemal microtubules.

Academic Significance and Societal Importance of the Research Achievements

本研究は、鞭毛のモデル生物クラミドモナスを用い、チューブリン・ポリグルタミン酸化修飾が鞭毛の構築にどのように関与するかを解明しようとするものである。ポリグルタミン酸化酵素TTLL6を欠失した新規の変異株ssa11を解析したところ、ssa11は既存のポリグルタミン酸化修飾変異株tpg1と二重変異にすると鞭毛の構築が顕著に遅くなることが分かった。このことは、ポリグルタミン酸化修飾が軸糸微小管の安定性に何らかの影響を及ぼしていることを示唆する。鞭毛繊毛は多くのヒト繊毛病に関わる。その構築機構を解明することは生物学的・基礎医学的に重要である。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (9 results)

All 2018 2017

All Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 4 results) Presentation (4 results) (of which Int'l Joint Research: 1 results,  Invited: 2 results)

  • [Journal Article] CFAP70 is a novel axoneme-binding protein that localizes at the base of the outer dynein arm and regulates ciliary motility2018

    • Author(s)
      Shamoto N, Narita K. Kubo T, Oda T, Takeda S.
    • Journal Title

      Cells

      Volume: 7 Issue: 9 Pages: 1-12

    • DOI

      10.3390/cells7090124

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Chlamydomonas as a tool to study tubulin polyglutamylation2018

    • Author(s)
      Kubo Tomohiro、Oda Toshiyuki
    • Journal Title

      Microscopy

      Volume: 68 Issue: 1 Pages: 80-91

    • DOI

      10.1093/jmicro/dfy044

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1)2018

    • Author(s)
      Kubo Tomohiro、Hou Yuqing、Cochran Deborah A.、Witman George B.、Oda Toshiyuki
    • Journal Title

      Molecular Biology of the Cell

      Volume: 29 Issue: 9 Pages: 1060-1074

    • DOI

      10.1091/mbc.e17-11-0689

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] RABL2 interacts with the intraflagellar transport-B complex and CEP19 and participates in ciliary assembly2017

    • Author(s)
      Nishijima Yuya、Hagiya Yohei、Kubo Tomohiro、Takei Ryota、Katoh Yohei、Nakayama Kazuhisa
    • Journal Title

      Molecular Biology of the Cell

      Volume: 28 Issue: 12 Pages: 1652-1666

    • DOI

      10.1091/mbc.e17-01-0017

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Electrostatic interaction between polyglutamylated tubulin and the nexin?dynein regulatory complex regulates flagellar motility2017

    • Author(s)
      Kubo Tomohiro、Oda Toshiyuki
    • Journal Title

      Molecular Biology of the Cell

      Volume: 28 Issue: 17 Pages: 2260-2266

    • DOI

      10.1091/mbc.e17-05-0285

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1).2018

    • Author(s)
      Tomohiro Kubo, Yuqing Hou, Deborah Cochran, George B. Witman, and Toshiyuki Oda.
    • Organizer
      第70 回日本細胞生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1)2018

    • Author(s)
      Kubo Tomohiro、Hou Yuqing、Cochran Deborah A.、Witman George B.、Oda Toshiyuki
    • Organizer
      The 18th International Conference on the Cell and Molecular Biology of Chlamydomonas
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Structural biology of Chlamydomonas flagella2017

    • Author(s)
      久保智広、小田賢幸
    • Organizer
      日本顕微鏡学会第73回学術講演会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] IFT81とIFT74 のN末端領域はチューブリン鞭毛内輸送のメインモジュールを形成する2017

    • Author(s)
      Tomohiro Kubo、Jason Brown, Karl Bellve, Branch Craige, Julie Craft, Kevin Forgarty, Karl Lechtreck, George Witman
    • Organizer
      第69回日本細胞生物学会大会
    • Related Report
      2017 Research-status Report
    • Invited

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Published: 2017-04-28   Modified: 2020-03-30  

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