Mechanisms of cell differentiation in the Arabidopsis root cap revealed by long-term time-lapse imaging
| Project/Area Number |
17K15140
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nara Institute of Science and Technology |
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Principal Investigator |
GOH Tatsuaki 奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (80511419)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 根冠 / 細胞内膜輸送 / タイムラプスイメージング / シロイヌナズナ / タイムラプス観察 / 細胞分化 / 植物 / 発生・分化 / 根 |
|---|
| Outline of Final Research Achievements |
The root cap consists of several layers of central columella and lateral root cap, and maintains its shape by balanced proliferation of proximal stem cells and autonomous release of distal mature cells. Time-lapse observation revealed dynamic subcellular rearrangements (vacuolization and shrinkage of amyloplasts) were operated in the outermost root cap layer during maturation and detachment. We also found that regulator protein of membrane traffic was involved in the subcellular rearrangement and root cap detachment.
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| Academic Significance and Societal Importance of the Research Achievements |
これまでに分子遺伝学的解析から根冠の分化や機能を制御する変異体や遺伝子が明らかにされてきたが，根冠の細胞層がどのようにして多様な機能を発揮しているのかは未解明なままであった。本研究において，長時間の連続観察から，根冠の細胞層が位置に応じて，構造と機能を大きく転換していることが明らかになった。このことは根冠機能の発現メカニズムについての理解を深めることに大きく貢献した。
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Lateral inhibition by a peptide hormone-receptor cascade during Arabidopsis lateral root founder cell formation.2019
Author(s)
Toyokura K, Goh T, Shinohara H, Shinoda A, Kondo Y, Okamoto Y, Uehara T, Fujimoto K, Okushima Y, Ikeyama Y, Nakajima K, Mimura T, Tasaka M, Matsubayashi Y and Fukaki H.
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Journal Title
Developmental Cell
Volume: 48
Issue: 1
Pages: 64-75
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Shaping 3D Root System Architecture2017
Author(s)
Morris Emily C.、Griffiths Marcus、Golebiowska Agata、Mairhofer Stefan、Burr-Hersey Jasmine、Goh Tatsuaki、von Wangenheim Daniel、Atkinson Brian、Sturrock Craig J.、Lynch Jonathan P.、Vissenberg Kris、Ritz Karl、Wells Darren M.、Mooney Sacha J.、Bennett Malcolm J.
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Journal Title
Current Biology
Volume: 27
Issue: 17
Pages: R919-R930
DOI
Related Report
Peer Reviewed / Int'l Joint Research
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