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Mechanisms of cell differentiation in the Arabidopsis root cap revealed by long-term time-lapse imaging

Research Project

Project/Area Number 17K15140
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Plant molecular biology/Plant physiology
Research InstitutionNara Institute of Science and Technology

Principal Investigator

GOH Tatsuaki  奈良先端科学技術大学院大学, 先端科学技術研究科, 助教 (80511419)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords根冠 / 細胞内膜輸送 / タイムラプスイメージング / シロイヌナズナ / タイムラプス観察 / 細胞分化 / 植物 / 発生・分化 / 根
Outline of Final Research Achievements

The root cap consists of several layers of central columella and lateral root cap, and maintains its shape by balanced proliferation of proximal stem cells and autonomous release of distal mature cells. Time-lapse observation revealed dynamic subcellular rearrangements (vacuolization and shrinkage of amyloplasts) were operated in the outermost root cap layer during maturation and detachment. We also found that regulator protein of membrane traffic was involved in the subcellular rearrangement and root cap detachment.

Academic Significance and Societal Importance of the Research Achievements

これまでに分子遺伝学的解析から根冠の分化や機能を制御する変異体や遺伝子が明らかにされてきたが,根冠の細胞層がどのようにして多様な機能を発揮しているのかは未解明なままであった。本研究において,長時間の連続観察から,根冠の細胞層が位置に応じて,構造と機能を大きく転換していることが明らかになった。このことは根冠機能の発現メカニズムについての理解を深めることに大きく貢献した。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (12 results)

All 2019 2018 2017

All Journal Article (5 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 5 results,  Open Access: 2 results) Presentation (7 results) (of which Int'l Joint Research: 1 results)

  • [Journal Article] Lateral inhibition by a peptide hormone-receptor cascade during Arabidopsis lateral root founder cell formation.2019

    • Author(s)
      Toyokura K, Goh T, Shinohara H, Shinoda A, Kondo Y, Okamoto Y, Uehara T, Fujimoto K, Okushima Y, Ikeyama Y, Nakajima K, Mimura T, Tasaka M, Matsubayashi Y and Fukaki H.
    • Journal Title

      Developmental Cell

      Volume: 48 Issue: 1 Pages: 64-75

    • DOI

      10.1016/j.devcel.2018.11.031

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Root branching toward water involves posttranslational modification of transcription factor ARF72018

    • Author(s)
      Orosa-Puente B、Leftley N、von Wangenheim D、Banda J、Srivastava AK.、Hill K、Truskina J、Bhosale R、Morris E、Srivastava M、Kmpers B、Goh T、Fukaki H、Vermeer JEM.、Vernoux T、Dinneny JR.、French AP.、Bishopp A、Sadanandom A、Bennett MJ.
    • Journal Title

      Science

      Volume: 362 Issue: 6421 Pages: 1407-1410

    • DOI

      10.1126/science.aau3956

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Long-term live-cell imaging approaches to study lateral root formation in Arabidopsis thaliana2018

    • Author(s)
      Goh Tatsuaki
    • Journal Title

      Microscopy

      Volume: 68 Issue: 1 Pages: 4-12

    • DOI

      10.1093/jmicro/dfy135

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Distinct sets of tethering complexes, SNARE complexes, and Rab GTPases mediate membrane fusion at the vacuole in Arabidopsis2018

    • Author(s)
      Takemoto Kodai、Ebine Kazuo、Askani Jana Christin、Kruger Falco、Gonzalez Zaida Andres、Ito Emi、Goh Tatsuaki、Schumacher Karin、Nakano Akihiko、Ueda Takashi
    • Journal Title

      Proc Natl Acad Sci USA.

      Volume: 115 Issue: 10

    • DOI

      10.1073/pnas.1717839115

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Shaping 3D Root System Architecture2017

    • Author(s)
      Morris Emily C.、Griffiths Marcus、Golebiowska Agata、Mairhofer Stefan、Burr-Hersey Jasmine、Goh Tatsuaki、von Wangenheim Daniel、Atkinson Brian、Sturrock Craig J.、Lynch Jonathan P.、Vissenberg Kris、Ritz Karl、Wells Darren M.、Mooney Sacha J.、Bennett Malcolm J.
    • Journal Title

      Current Biology

      Volume: 27 Issue: 17 Pages: R919-R930

    • DOI

      10.1016/j.cub.2017.06.043

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] シロイヌナズナ根冠細胞の剥離過程における周期的な細胞挙動の解析2019

    • Author(s)
      郷達明,上野皓輝,宮島俊介,中島敬二
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Unravelling temporally coordinated cell divisions in the Arabidopsis root meristem by a motion-tracking microscope system2019

    • Author(s)
      Katsutoshi Imizu,Shunsuke Miyashima,Tatsuaki Goh,Keiji Nakajima
    • Organizer
      第60回日本植物生理学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 根冠細胞の分化過程における細胞内膜輸送経路の役割2018

    • Author(s)
      郷達明, 小堤彩水, 小園紗希, 金錘明, 遠藤高帆, 宮島俊介, 中島敬二
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] シロイヌナズナRKD4による初期胚発生制御機構の解析2018

    • Author(s)
      渡部貴史, 金錘明, 遠藤高帆, 郷達明, 中島 敬二
    • Organizer
      日本植物学会第82回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 長時間連続観察による根冠細胞の剥離様式の解析2017

    • Author(s)
      郷達明,宮島俊介,上野皓輝,中島敬二
    • Organizer
      日本植物学会第81回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Mechanisms of root cap maturation and cell detachment in Arabidopsis2017

    • Author(s)
      Goh T, Ueno K, Kamiya M, Miyashima S, Nakajima K
    • Organizer
      Bilateral Closure Symposium of GDRI Integrative Plant Biology Network Program
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 長時間連続観察によるシロイヌナズナ根冠細胞の周期的な剥離様式の解析2017

    • Author(s)
      郷達明,上野皓輝,宮島俊介,中島敬二
    • Organizer
      第59回日本植物生理学会年会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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