Genetic basis for parallel evolution of selfing in green algae

• Project/Area Number: 17K15165
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Evolutionary biology
• Research Institution: Chiba University
• Principal Investigator: Tsuchimatsu Takashi 千葉大学, 大学院理学研究院, 准教授 (60740107)
• Research Collaborator: TSUCHIKANE Yuki ; SEKIMOTO Hiroyuki ; NISHIYAMA Tomoaki ; KAWAGUCHI Yawako
• Project Period (FY): 2017-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
• Keywords: 自家交配 / 比較ゲノム / 自殖 / 交配様式 / 生殖システム / 集団ゲノム

Outline of Final Research Achievements

In this study, we investigated the genomic consequences of evolutionary transitions between heterothallism and homothallism in Closterium peracerosum-strigosum-littorale complex, using the genome-wide polymorphism data from many wild samples. We revealed (1) recurrent transitions between mating types, (2) frequent segmental duplications particularly in homothallic strains, and (3) more accumulations of deleterious mutations in homothallic strains.

Academic Significance and Societal Importance of the Research Achievements

自殖の進化は，何度も繰り返し起きた平行進化の好例である．繰り返し起きた自殖がどのような遺伝メカニズムにより生じ，ゲノムにどのような影響を与えるのかといった問題については，今まで被子植物における研究が主だった．本研究で，接合藻類ヒメミカヅキモにおける自殖進化の遺伝的背景を研究する基盤が整ったことで，自殖の平行進化をより広い分類群で解明し，適応進化にみられる普遍性を検討することができるようになると考えられる．

Research Products

• [Journal Article] Exploring genetic diversity and signatures of horizontal gene transfer in nodule bacteria associated with Lotus japonicus in natural environments (2019)
  • Author(s): Bamba, M., Aoki, S., Kajita, T., Setoguchi H., Watano, Y., Sato, S., and Tsuchimatsu, T.
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 印刷中 Issue: 9 Pages: 1110-1120
  • DOI: 10.1094/mpmi-02-19-0039-r
  • Peer Reviewed / Open Access
• [Journal Article] Plant adaptation and speciation studied by population genomic approaches (2019)
  • Author(s): Bamba Masaru、Kawaguchi Yawako W.、Tsuchimatsu Takashi
  • Journal Title: Development, Growth & Differentiation Volume: 61 Issue: 1 Pages: 12-24
  • DOI: 10.1111/dgd.12578
  • Peer Reviewed / Open Access
• [Journal Article] Evolutionary Dynamics of Plant Populations Studied by Population Genomics: Genomics of Adaptation (2018)
  • Author(s): TSUCHIMATSU Takashi
  • Journal Title: KAGAKU TO SEIBUTSU Volume: 56 Issue: 5 Pages: 317-323
  • DOI: 10.1271/kagakutoseibutsu.56.317
  • NAID: 130007635167
  • ISSN: 0453-073X, 1883-6852
  • Year and Date: 2018-04-20
  • Peer Reviewed
• [Journal Article] NLR locus-mediated trade-off between abiotic and biotic stress adaptation in Arabidopsis (2017)
  • Author(s): Ariga Hirotaka、Katori Taku、Tsuchimatsu Takashi、Hirase Taishi et al.
  • Journal Title: Nature Plants Volume: 3 Issue: 6 Pages: 17072-17072
  • DOI: 10.1038/nplants.2017.72
  • NAID: 120006309713
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] African genomes illuminate the early history and transition to selfing inArabidopsis thaliana (2017)
  • Author(s): Durvasula Arun、Fulgione Andrea、Gutaker Rafal M.、Alacakaptan Selen Irez、Flood Pedraic J.、Neto Celia、Tsuchimatsu Takashi、Burbano Hernan A.、Pico F. Xavier、Alonso-Blanco Carlos、Hancock Angela M.
  • Journal Title: PNAS Volume: 114 Issue: 20 Pages: 5213-5218
  • DOI: 10.1073/pnas.1616736114
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Patterns of polymorphism at the self-incompatibility locus in 1,083 Arabidopsis thaliana genomes. (2017)
  • Author(s): Tsuchimatsu, T., Goubet, P.M., Gallina, S., Holl, A.C., Fobis-Loisy, I., Berges, H., Marande, W., Prat, E., Meng, D., Long, Q., Platzer, A., Nordborg, M., Vekemans, X., Castric, V.
  • Journal Title: Molecular Biology and Evolution Volume: 印刷中 Issue: 8 Pages: 1878-1889
  • DOI: 10.1093/molbev/msx122
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Genome sequencing reveals the origin of the allotetraploid Arabidopsis suecica. (2017)
  • Author(s): Novikova, P.Y., Tsuchimatsu, T., Simon, S., Nizhynska, V., Voronin, V., Burns, R., Fedorenko, O.M., Holm, S., Sall, T., Prat, E., Marande, W., Castric, V. and Nordborg, M.
  • Journal Title: Molecular Biology and Evolution Volume: 34 Pages: 957-968
  • DOI: 10.1093/molbev/msw299
  • Peer Reviewed / Int'l Joint Research
• [Journal Article] Population genomics reveals evolutionary processes of plant populations (2017)
  • Author(s): 土松隆志
  • Journal Title: Leading Author's Volume: 6 Pages: e006
  • DOI: 10.7875/leading.author.6.e006
  • Peer Reviewed
• [Presentation] 自家受精の平行進化のゲノム基盤 (2019)
  • Author(s): 土松隆志
  • Organizer: 神戸大学大学院農学研究科インターゲノミクスセミナー
  • Invited
• [Presentation] 植物の適応形質のゲノム科学 (2018)
  • Author(s): 土松隆志
  • Organizer: 京都大学生態学研究センター研究集会「異なるマクロ生物学分野のインタープレイ」
  • Invited
• [Presentation] アブラナ科植物における自家不和合性と自家受精の進化 (2018)
  • Author(s): 土松隆志
  • Organizer: 日本育種学会ワークショップ「アブラナ科植物における自家不和合性研究の最前線と育種現場での利用」
  • Invited
• [Presentation] 南米産ペチュニア属植物の自家和合系統を用いたS遺伝子座の配列解析 (2018)
  • Author(s): 坂本奈緒子，渡辺均，上原浩一，土松隆志
  • Organizer: 日本植物学会
• [Presentation] 接合藻ヒメミカヅキモ種内系統間にみられるゲノム構造の変異：生殖様式の進化との関連 (2018)
  • Author(s): 川口也和子，土金勇樹，西山智明，関本弘之，土松隆志
  • Organizer: 日本植物学会
• [Presentation] 植物における自家不和合性と自家受精の進化：アブラナ科・ナス科植物を例に (2018)
  • Author(s): 土松隆志
  • Organizer: 日本進化学会シンポジウム「生態発生進化」
  • Invited
• [Presentation] 南米産ペチュニア属植物を用いた自家不和合性遺伝子座配列解析 (2018)
  • Author(s): 坂本奈緒子，渡辺均，上原浩一，土松隆志
  • Organizer: 日本進化学会
• [Presentation] 2種類の生殖様式を持つ接合藻ヒメミカヅキモにおけるゲノム構造の系統間比較 (2018)
  • Author(s): 川口也和子，土金勇樹，西山智明，関本弘之，土松隆志
  • Organizer: 日本進化学会
• [Presentation] 全ゲノム配列解析と交互接種実験から探るマメ科植物-根粒菌間の共生特異性の進化 (2018)
  • Author(s): 番場大，青木誠志朗，梶田忠，瀬戸口浩彰，綿野泰行，佐藤修正，土松隆志
  • Organizer: 日本進化学会
• [Presentation] Diversity and horizontal gene transfer of nodule bacteria associated with Lotus japonicus in natural environments (2018)
  • Author(s): Bamba, M., Aoki, S., Kajita, T., Watano, Y., Setoguchi, H., Sato, S., Tsuchimatsu, T.
  • Organizer: Annual Meeting of the Society for Molecular Biology and Evolution
  • Int'l Joint Research
• [Presentation] 植物における自家不和合性と自家受精の進化 (2018)
  • Author(s): 土松隆志
  • Organizer: 遺伝学研究所研究会「マクロ生態学と遺伝学の融合」
  • Invited
• [Presentation] ゲノムから探るシロイヌナズナにおける生殖システムの進化 (2018)
  • Author(s): 土松 隆志
  • Organizer: 総合研究大学院大学 先導科学考究
  • Invited
• [Presentation] シロイヌナズナの集団ゲノムデータから探る生殖システムの進化 (2017)
  • Author(s): 土松 隆志
  • Organizer: 東北大学 第117回生態適応セミナー
  • Invited
• [Presentation] 集団ゲノミクスから探る生殖システムの進化：シロイヌナズナを例に (2017)
  • Author(s): 土松 隆志
  • Organizer: 東京大学大学院理学系研究科生物科学セミナー
  • Invited
• [Presentation] 1001ゲノムデータから探るシロイヌナズナ自家不和合性遺伝子座の進化過程 (2017)
  • Author(s): 土松 隆志，Goubet PM, Nordborg M, Vekemans X, Castric
  • Organizer: 日本植物学会第81回大会
• [Presentation] リシーケンスデータから探るシロイヌナズナ自家不和合性遺伝子座の進化過程 (2017)
  • Author(s): 土松 隆志，Goubet PM, Nordborg M, Vekemans X, Castric, V
  • Organizer: 日本進化学会第19回大会
• [Presentation] Population genomics reveals the mating system evolution in Arabidopsis (2017)
  • Author(s): Tsuchimatsu T
  • Organizer: The 5th International Symposium “Frontier Science in Ecology, Genetics and Genomics
  • Int'l Joint Research / Invited
• [Presentation] Patterns of polymorphism at the self-incompatibility locus in 1,083 Arabidopsis thaliana genomes (2017)
  • Author(s): Tsuchimatsu T, Goubet PM, Nordborg M, Vekemans X, Castric, V
  • Organizer: Cold Spring Harbor Asia Conference on Plant Cell and Developmental Biology
  • Int'l Joint Research
• [Book] 分子生物学 (2018)
  • Author(s): J. Zlatanova、K. E. van Holde、田村 隆明
  • Total Pages: 608
  • Publisher: 東京化学同人
  • ISBN: 9784807909490
• [Book] 植物はなぜ自家受精をするのか (2017)
  • Author(s): 土松 隆志
  • Total Pages: 170
  • Publisher: 慶應義塾大学出版会
  • ISBN: 4766422996
• [Book] 科学の最前線を歩く (2017)
  • Author(s): 東京大学教養学部
  • Total Pages: 291
  • Publisher: 白水社
  • ISBN: 4560095639
• [Remarks] Tsuchimatsu Lab
  • URL: https://tsuchimatsu.wordpress.com
• [Remarks] Tsuchimatsu Lab
  • URL: https://tsuchimatsu.wordpress.com/