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Developing of the radioisotope-imaging system for plants

Research Project

Project/Area Number 17K15355
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Agricultural environmental engineering/Agricultural information engineering
Research InstitutionThe University of Tokyo

Principal Investigator

Sugita Ryohei  東京大学, 大学院農学生命科学研究科(農学部), 助教 (60724747)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords放射線イメージング / 植物栄養 / シンチレータ / ベータ線 / RIイメージング / β線 / 蛍光イメージング / 放射線計測 / 植物 / 栄養元素 / ライブイメージング / 元素輸送 / リアルタイムRIイメージング / 菌根菌
Outline of Final Research Achievements

In this study, we have improved a real-time radioisotope imaging system for taking fluorescence imaging. The method is to perform LED lighting for plants, pulsed fluorescence for fluorescence imaging, and radioisotope imaging during the dark period. The photosynthetic capacity of plants is reduced when pulse lighting is provided at intervals shorter than 50 msec. Therefore, we constructed a system that synchronizes a microcontroller board and a CCD camera to capture images at intervals of 50 msec. The lighting system was successfully controlled of lighting for plants and taking images in msec increments.

Academic Significance and Societal Importance of the Research Achievements

高度な照明システムの構築により、様々な蛍光イメージングを同時に撮影できる見通しである。本システムは他のRIイメージングにも適用可能である。また、視野範囲の拡大にも成功した。これまでは20 cm x 10 cmの視野範囲であり、幼植物のみを解析の対象としてきたが、新たにシンチレータを改良したことで、80 cm x 60 cmの撮影が可能になった。これにより、イネやダイズの収穫期ステージの試料をも撮影の対象になりうる。今後は作物の子実に輸送される養分がどのように輸送されていくのかを解析していくことで、収量増加や施肥の最適化を目指す。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (8 results)

All 2020 2019 2018 2017 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (5 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Remarks (1 results)

  • [Journal Article] Plastic Scintillators Enable Live Imaging of <sup>32</sup>P-labelled Phosphorus Movement in Large Plants2019

    • Author(s)
      Kohei Sugahara, Ryohei Sugita, Natsuko I. Kobayashi, Atsushi Hirose, Tomoko M. Nakanishi, Etsuko Furuta, Masaaki Sensui, Keitaro Tanoi
    • Journal Title

      RADIOISOTOPES

      Volume: 68 Issue: 3 Pages: 73-82

    • DOI

      10.3769/radioisotopes.68.73

    • NAID

      130007614642

    • ISSN
      0033-8303, 1884-4111
    • Year and Date
      2019-03-15
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluation of plastic scintillators for live imaging of 14C-labeled photosynthate movement in plants2018

    • Author(s)
      Ryohei SugitaKohei SugaharaNatsuko I. KobayashiAtsushi HiroseTomoko M. NakanishiEtsuko FurutaMasaaki SensuiKeitaro Tanoi
    • Journal Title

      Journal of Radioanalytical and Nuclear Chemistry

      Volume: 318 Issue: 1 Pages: 579-584

    • DOI

      10.1007/s10967-018-6102-z

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Presentation] 植物体内イオンの可視化に向けたRIイメージング装置の開発2020

    • Author(s)
      杉田亮平
    • Organizer
      第67回応用物理学会春季学術講演会
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] RIライブイメージングを用いた植物体内イオンの可視化2019

    • Author(s)
      杉田亮平
    • Organizer
      Optics & Photonics Japan 2019
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] リアルタイムRIイメージングシステム(RRIS)を用いた元素動態の可視化技術の開発2019

    • Author(s)
      杉田亮平,小川隆宏,廣瀬農,小林奈通子,中西友子,田野井慶太朗
    • Organizer
      第56 回 アイソトープ・放射線研究発表会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Live imaging of ion movement in plants by Real-Time Radioisotope Imaging System (RRIS)2017

    • Author(s)
      Sugita, R.; Kobayashi, N.I.; Hirose, A.; Tanoi, K.; Nakanishi, T.M.
    • Organizer
      IPNC 2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Live imaging of photosynthate and ion movement in plants using Real-Time Radioisotope Imaging System (RRIS)2017

    • Author(s)
      Sugita, R.; Kobayashi, N.I.; Hirose, A.; Tanoi, K.; Nakanishi, T.M.
    • Organizer
      IGER International Symposium
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Remarks] 放射線植物生理学研究室

    • URL

      http://park.itc.u-tokyo.ac.jp/radio-plantphys/index.html

    • Related Report
      2019 Annual Research Report 2018 Research-status Report 2017 Research-status Report

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Published: 2017-04-28   Modified: 2021-02-19  

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