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Analysis of fetal-maternal interaction in hypoxic environment

Research Project

Project/Area Number 17K15357
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Animal production science
Research InstitutionHokkaido University

Principal Investigator

Bai Hanako  北海道大学, 農学研究院, 助教 (60775443)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywords子宮 / ウシ / 低酸素 / 反芻動物 / 遺伝子発現 / 胚
Outline of Final Research Achievements

This study focused on the difference between oxygen concentration in the atmosphere and in utero environment. The objective of this study are: 1. Establishment of hypoxic cell culture system using bovine embryo and endometrial cells, 2. Identification and analysis of important candidate factors of bovine embryo and endometrial cells, and 3. Construction of hypoxic response pathway(s) necessary for pregnancy establishment.
1. I tried culture using bovine embryo and trophoblast cell CT-1 and F3 cells, but we could not stably maintain. Endometrial cells were compared in normal and hypoxic conditions. With regard to 2, and 3, I focused on only endometrial epithelial cells. Now I examine expression analysis of protein levels under hypoxic conditions and are investigating candidate factors.

Academic Significance and Societal Importance of the Research Achievements

本研究では、通常は大気中と同じ条件で行っている細胞培養実験を子宮内環境を想定した酸素濃度での行い、その中で発現変動する候補因子を解析した。まだ精査が必要な段階であるが、これら因子およびその機能の検証により、妊娠成立のための知見が得られることが期待できる。得られる結果は新規知見であるため学術的意義は大きく、現在私たちヒトにおいても不妊は社会的問題となっていることからその社会的意義も大きいと考えている。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (14 results)

All 2019 2018 2017

All Journal Article (11 results) (of which Peer Reviewed: 11 results,  Open Access: 5 results) Presentation (3 results)

  • [Journal Article] Evaluation of the immune status of peripheral blood monocytes from dairy cows during the periparturition period.2019

    • Author(s)
      Bai H, Talukder MAS, Kunii H, Itoh T, Kawahara M, Takahashi M.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 65

    • NAID

      130007689002

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Overgrowth of mice generated from postovulatory aged oocyte spindles.2019

    • Author(s)
      Shiina K, Komatsu M, Yokoi F, Bai H, Takahashi T, Kawahara M.
    • Journal Title

      FASEB BioAdvances

      Volume: 印刷中 Issue: 7 Pages: 1-11

    • DOI

      10.1096/fba.2019-00005

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Significance of CCN2 expression in bovine preimplantation development2018

    • Author(s)
      Akizawa Hiroki、Yanagawa Yojiro、Nagano Masashi、Bai Hanako、Takahashi Masashi、Kawahara Manabu
    • Journal Title

      Animal Science Journal

      Volume: 90 Issue: 1 Pages: 49-54

    • DOI

      10.1111/asj.13126

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Involvement of interferon-tau in the induction of apoptotic, pyroptotic, and autophagic cell death-related signaling pathways in the bovine uterine endometrium during early pregnancy2018

    • Author(s)
      Suzuki T, Sakumoto R, Hayashi K, Ogiso T, Kunii H, Shirozu T, Kim SW, Bai H, Kawahara M, Kimura K, Takahashi M.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 64 Issue: 6 Pages: 495-502

    • DOI

      10.1262/jrd.2018-063

    • NAID

      130007530502

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pregnancy-induced expression of interferon-stimulated genes in cervical and vaginal mucosal membrane.2018

    • Author(s)
      Kunii H, Koyama K, Ito T, Suzuki T, Balboula AZ, Shirozu T, Bai H, Nagano M, Kawahara M, Takahashi M.
    • Journal Title

      Journal of Dairy Science

      Volume: 101 Issue: 9 Pages: 8396-8400

    • DOI

      10.3168/jds.2017-14251

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Reciprocal regulation of TEAD4 and CCN2 for the trophectoderm development of the bovine blastocyst.2018

    • Author(s)
      Hiroki Akizawa, Ken Kobayashi , Hanako Bai, Masashi Takahashi, Shinjiro Kagawa, Hiroaki Nagatomo, Manabu Kawahara
    • Journal Title

      Reproduction

      Volume: 印刷中 Issue: 6 Pages: 563-571

    • DOI

      10.1530/rep-18-0043

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Identification and expression analysis of cDNA encoding insulin-like growth factor 2 in horses2018

    • Author(s)
      Kikuchi K, Sasaki K, Akizawa H, Tsukahara H, Bai H, Takahashi M, Nambo Y, Hata H, Kawahara M.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 64 Issue: 1 Pages: 57-64

    • DOI

      10.1262/jrd.2017-124

    • NAID

      130006405024

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Evaluating the electrical impedance and mucus-related gene expression of uterine endometrial tissues in mares2018

    • Author(s)
      Kikuchi K, Kozai K, Hojo T, Sakatani M, Okuda K, Bai H, Kawahara M, Takahashi M.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 64 Issue: 2 Pages: 193-197

    • DOI

      10.1262/jrd.2017-128

    • NAID

      130006699687

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Maternal recognition and establishment of pregnancy in ruminants2018

    • Author(s)
      Bai H, Kawahara M, Takahashi M
    • Journal Title

      Reviews on Animal Nutrition and Metabolism

      Volume: 62 Pages: 19-25

    • NAID

      40021510403

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Novel endogenous retrovirus-derived transcript expressed in the bovine placenta is regulated by WNT signaling.2017

    • Author(s)
      Sakurai T, Nakagawa S, Bai H, Bai R, Kusama K, Ideta A, Aoyagi Y, Kaneko K, Iga K, Yasuda J, Miyazawa T, Imakawa K.
    • Journal Title

      Biochemical Journal

      Volume: 474 Issue: 20 Pages: 3499-3512

    • DOI

      10.1042/bcj20170531

    • NAID

      120006649525

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Enhancement of sperm motility and viability by turmeric by-product dietary supplementation in roosters2017

    • Author(s)
      Yan W, Kanno C, Oshima E, Kuzuma Y, Kim SW, Bai H, Takahashi M, Yanagawa Y, Nagano M, Wakamatsu JI, Kawahara M.
    • Journal Title

      Animal reproduction science.

      Volume: 185 Pages: 195-204

    • DOI

      10.1016/j.anireprosci.2017.08.021

    • NAID

      120006523735

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] ウシ子宮内膜上皮細胞を用いた暑熱負荷培養およびインターフェロン・タウ応答性の検証2018

    • Author(s)
      小松 正明,鈴木 惇文,国井 宏樹,川原 学,木村 康二,高橋 昌志,唄 花子
    • Organizer
      日本繁殖生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 乳牛の分娩前後における末梢血単核球のストレスおよび免疫関連因子の解析2018

    • Author(s)
      唄 花子,Shabur Md Abdus Talukder,国井 宏樹,伊藤 月乃,川原 学,高橋 昌志
    • Organizer
      日本繁殖生物学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 反芻動物の妊娠認識と成立機構.2018

    • Author(s)
      唄花子、川原学、高橋昌志.
    • Organizer
      家畜栄養生理研究会
    • Related Report
      2017 Research-status Report

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Published: 2017-04-28   Modified: 2020-03-30  

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