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Development of method for central nervous regeneration using stem cell-derived exosomes

Research Project

Project/Area Number 17K15378
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionOsaka Prefecture University (2018)
Gifu University (2017)

Principal Investigator

Nishida Hidetaka  大阪府立大学, 生命環境科学研究科, 准教授 (00622804)

Research Collaborator MAEDA Sadatoshi  岐阜大学, 応用生物科学部, 教授 (50377694)
KAMISHINA Hiroaki  岐阜大学, 応用生物科学部, 准教授 (50506847)
AKIYOSHI Hideo  大阪府立大学, 生命環境科学研究科, 教授 (50420740)
HATOYA Shingo  大阪府立大学, 生命環境科学研究科, 准教授 (40453138)
Prockop Darwin  Texas A&M Health Science Center, Professor
Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2018: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywordsエクソソーム / 間葉系幹細胞 / 中枢神経再生 / トランスレーショナルリサーチ / 再生医療 / 幹細胞 / 分離 / 抗炎症作用 / 移植・再生医療 / 脳・神経 / 臨床
Outline of Final Research Achievements

we developed that mesenchymal stem/stromal cells can be activated by incubation for 48 hours in a chemically defined and protein-free medium to produce stem cell-derived exosomes. We developed a scalable chromatographic protocol for isolating exosomes from canine and human mesenchymal stem/stromal cells. These exosomes reduced neuroinflammation as reflected by reductions in IL-1β. The results suggest that canine and human mesenchymal stem/stromal cells-derived exosomes can provide a novel therapy for central nervous system diseases.

Academic Significance and Societal Importance of the Research Achievements

本研究では、 培養上清中に存在するエクソソームに着目し、精製後に凍結保存することによって “ready-to-use”を実現可能にした新たな再生医療の治療技術の提供が実現可能となった。本研究で得られた結果を総合的に評価し、次のターゲットであるイヌの中枢神経損傷に対する臨床研究につなげていく予定である。イヌの中枢神経損傷症例は自然発症疾患であり、ヒトのト ランスレーショナルリサーチモデルとして重要な役割を担っている。本研究は伴侶動物の再生医 療を推進させるだけでなく、ヒト臨床応用への有効性を検討するための信頼性の高い情報を提供することが可能となる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (11 results)

All 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (8 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Remarks (1 results)

  • [Int'l Joint Research] Texas A&M University/University of California, Davis(米国)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Identification of reference genes for microRNAs of extracellular vesicles isolated from plasma samples of healthy dogs by ultracentrifugation, precipitation, and membrane affinity chromatography methods2019

    • Author(s)
      Narita M, Nishida H, Asahina R, Nakata K, Yano H, Ueda T, Inden M, Akiyoshi H, Maeda S, Kamishima H
    • Journal Title

      American Journal of Veterinary Research

      Volume: in press

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] 無血清培地におけるイヌ骨髄間葉系幹細胞由来Extracellular Vesicleの回収法の検討2019

    • Author(s)
      吉﨑香琳、西田英高、桑原由季菜、三重慧一郎、秋吉秀保、城潤一郎、田畑泰彦
    • Organizer
      第18回日本再生医療学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Development of novel therapy for spinal cord injury using extracellular vesicles derived from mesenchymal stem/stromal cells in dogs2018

    • Author(s)
      Nishida H, Yoshiazaki K, Kuwahara Y, Mie K, Akiyoshi H
    • Organizer
      8th Annual Congress of Asia Society of Veterinary Surgery
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 幹細胞由来Extracellular Vesicleを用いた中枢神経治療法の開発2018

    • Author(s)
      西田英高、吉﨑香琳、桑原由季菜、三重慧一郎、秋吉秀保、Kim Dong-Ki、Prockop Darwin
    • Organizer
      第8回DDS再生医療研究会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Clinical application and future directions of mesenchymal stem/stromal cell therapy for spinal cord injury in dogs2018

    • Author(s)
      Nishida H
    • Organizer
      2018 World Congress of Tissue Engineering and Regenerative Medicine International Society
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 犬の頚部椎間板ヘルニアに対するMRI伸延撮像法の検討2018

    • Author(s)
      西田英高、中田浩平、山﨑緑、中野有希子、田中宏、中山正成、秋吉秀保、前田貞俊、神志那弘明
    • Organizer
      第 44 回獣医神経病学会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 幹細胞由来液性因子を用いた治療戦略2018

    • Author(s)
      西田英高
    • Organizer
      第13回日本獣医再生医療学会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 脊髄再生の道のりと今後の展望2018

    • Author(s)
      西田英高
    • Organizer
      第13回日本獣医再生医療学会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] qRT-PCRを用いた犬の血漿エクソソームmiRNA定量法の確立2017

    • Author(s)
      成田桃子、西田英高、朝比奈良太、中田浩平、矢野大仁、前田貞俊、神志那弘明
    • Organizer
      第4回日本細胞外小胞学会/第9回日本RNAi研究会
    • Related Report
      2017 Research-status Report
  • [Remarks] 大阪府立大学 獣医外科教室

    • URL

      http://www.vet.osakafu-u.ac.jp/surg/

    • Related Report
      2018 Annual Research Report

URL: 

Published: 2017-04-28   Modified: 2020-03-30  

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