| Project/Area Number |
17K15538
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Hirosaki University |
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Principal Investigator |
Okano Daisuke 弘前大学, 医学研究科, 助手 (00719023)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | リンパ管 / 三次元培養 / CD73 / ライブイメージング / 形態形成 / 細胞分化・組織形成 / 形態形成運動 |
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| Outline of Final Research Achievements |
We showed that a primary lymphatic vessels network is formed on a plane in a three-dimensional lymphatic vessel tissue model. And then lymphangiogenesis occurs from there, and budding, elongation, migration, and anastomosis are repeated to form a secondary lymphatic vessels network. Intracellular vesicles of lymphatic endothelial cells changed into lymphatic lumen during lymphangiogenesis. It was suggested that CD73 expression and adenosine receptor expression of fibroblasts in a three-dimensional lymphatic vessel model are involved in the lymphatic network formation ability.
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| Academic Significance and Societal Importance of the Research Achievements |
リンパ管新生はがんのリンパ行性転移だけでなく免疫、むくみやアンチエイジングなど様々な場面での関与があるにもかかわらず不明な点が多い。本研究ではタイムラプス・ライブイメージングでリンパ管形態形成を明らかにし、線維芽細胞のCD73がリンパ管ネットワーク形成に関与していることが示唆された。がんの病態解明や、ひいては新たな治療戦略の確立に寄与することが期待される。
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