• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effect of redox metabolic flux regulated by cancer metabolism on anticancer drug resistance

Research Project

Project/Area Number 17K15673
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Experimental pathology
Research InstitutionNippon Medical School

Principal Investigator

Ishino Kousuke  日本医科大学, 医学部, 講師 (60584878)

Project Period (FY) 2017-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Keywords2-デオキシグルコース / 膵がん細胞株 / メトホルミン / がん代謝 / 併用投与 / 相乗効果 / 抗がん剤耐性 / 抗酸化代謝物 / 2-デオキシ-D-グルコース(2DG) / 抗がん剤感受性 / MIAPaCa2 細胞 / A549細胞 / シスプラチン / N-アセチルシステイン(NAC) / 活性酸素種(ROS) / 酸化ストレス / ドライバー遺伝子
Outline of Final Research Achievements

Many cancer cells also exert resistance to the action of anti-cancer agents because they reprogram metabolism in a glycolytic-dominant manner and respond to adverse environments. The glucose analog 2-deoxyglucose (2DG) not only inhibits glycolysis and suppresses the growth of cancer cells, but also enhances the action of drugs such as anticancer agents. In the present study, we found that 2DG synergistically enhanced the action of cisplatin or metformin on pancreatic cancer cells. It was also found that the combination of 2DG and metabolite suppressed the action of 2DG on cells. It is expected that the cancer treatment will be further advanced by further understanding the action of 2DG.

Academic Significance and Societal Importance of the Research Achievements

本研究は、がんが栄養の使い方を変化させてブドウ糖を多量に使うようになっている性質を利用して新しい薬の候補を調べた研究です。がん細胞がブドウ糖を使うのを防ぐ2DGという化合物を、抗がん剤などの薬(シスプラチンやメトホルミン)と一緒にがん細胞に加えると、薬の効き目が高くなることがわかりました。この飲み合わせが効果的な理由を調べることで、いろいろながんに効く薬を作るヒントを調べられる可能性があります。

Report

(4 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • Research Products

    (18 results)

All 2019 2018 2017 Other

All Journal Article (6 results) (of which Peer Reviewed: 6 results,  Open Access: 3 results) Presentation (10 results) (of which Int'l Joint Research: 1 results) Remarks (2 results)

  • [Journal Article] Farnesoid X receptor induces cell death and sensitizes to TRAIL-induced inhibition of growth in colorectal cancer cells through the up-regulation of death receptor 5.2019

    • Author(s)
      Hotta M, Sakatani T, Ishino K, Wada R, Kudo M, Yokoyama Y, Yamada T, Yoshida H, Naito Z
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 519 Issue: 4 Pages: 824-831

    • DOI

      10.1016/j.bbrc.2019.09.033

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of protein disulfide isomerase A3 and its clinicopathological association in gastric cancer2019

    • Author(s)
      Shimoda Tomohiro、Wada Ryuichi、Kure Shoko、Ishino Kousuke、Kudo Mitsuhiro、Ohashi Ryuji、Fujita Itsuo、Uchida Eiji、Yoshida Hiroshi、Naito Zenya
    • Journal Title

      Oncology Reports

      Volume: 41 Pages: 2265-2272

    • DOI

      10.3892/or.2019.6999

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Downregulation of protein disulfide-isomerase A3 expression inhibits cell proliferation and induces apoptosis through STAT3 signaling in hepatocellular carcinoma2019

    • Author(s)
      Kondo R, Ishino K, Wada R, Takata H, Peng WX, Kudo M, Kure S, Kaneya Y, Taniai N, Yoshida H, Naito Z
    • Journal Title

      International Journal of Oncology

      Volume: 54 Pages: 1409-1421

    • DOI

      10.3892/ijo.2019.4710

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] Nuclear Morphological Changes in Papillary Thyroid Carcinoma Cell: The Utility of a 3-Dimensional (3D) Holographic Microscopy in Cytology2018

    • Author(s)
      Kure Shoko、Kudo Mitsuhiro、Ishino Kousuke、Wada Ryuichi、Naito Zenya
    • Journal Title

      Journal of Cytology & Histology

      Volume: 09 Issue: 06 Pages: 527-527

    • DOI

      10.4172/2157-7099.1000527

    • Related Report
      2018 Research-status Report
    • Peer Reviewed
  • [Journal Article] 2-Deoxy-D-glucose increases GFAT1 phosphorylation resulting in endoplasmic reticulum-related apoptosis via disruption of protein N-glycosylation in pancreatic cancer cells2018

    • Author(s)
      Ishino K, Kudo M, Peng WX, Kure S, Kawahara K, Teduka K, Kawamoto Y, Kitamura T, Fujii T, Yamamoto T, Wada R, Naito Z
    • Journal Title

      Biochem Biophys Res Comm

      Volume: 501 Issue: 3 Pages: 668-673

    • DOI

      10.1016/j.bbrc.2018.05.041

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Expression of DNA damage response proteins in gastric cancer: Comprehensive protein profiling and histological analysis2018

    • Author(s)
      Arai Hiroki、Wada Ryuichi、Ishino Kousuke、Kudo Mitsuhiro、Uchida Eiji、Naito Zenya
    • Journal Title

      International Journal of Oncology

      Volume: 52 Pages: 978-988

    • DOI

      10.3892/ijo.2018.4238

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] 質量分析データに基づくDNA付加体データベースの整備2018

    • Author(s)
      石野孔祐、前迫侑也、内藤善哉、戸塚ゆ加里
    • Organizer
      日本環境変異原学会 第47回大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 膵癌細胞株において2-デオキシ-D-グルコースはGFAT1リン酸化を介して小胞体ストレス関連細胞死を誘発する2018

    • Author(s)
      石野孔祐、工藤光洋、彭為霞、呉壮香、和田龍一、内藤善哉
    • Organizer
      第77回日本癌学会学術総会
    • Related Report
      2018 Research-status Report
  • [Presentation] 有棘細胞癌におけるTLR4の発現と機能についての検討2018

    • Author(s)
      三神絵理奈、工藤光洋、大橋隆治、川原清子、河本陽子、手塚潔、藤井雄文、呉壮香、石野孔祐、坂谷貴司、和田龍一、佐伯秀久、内藤善哉
    • Organizer
      第77回日本癌学会学術総会
    • Related Report
      2018 Research-status Report
  • [Presentation] 肝細胞癌におけるSTAT3経路を介したPDIA3による細胞増殖とアポトーシスの制御2018

    • Author(s)
      近藤亮太、石野孔祐、和田龍一、工藤光洋、内藤善哉
    • Organizer
      第77回日本癌学会学術総会
    • Related Report
      2018 Research-status Report
  • [Presentation] 40歳未満の乳頭癌におけるBRAF遺伝子変異と臨床病理学的因子の関連についての検討2018

    • Author(s)
      呉壮香、和田龍一、彭為霞、石野孔祐、工藤光洋、杉谷巌、内藤善哉
    • Organizer
      第6回日本甲状腺病理学会総会・学術総会
    • Related Report
      2018 Research-status Report
  • [Presentation] 肝細胞癌においてPDIA3はJak/STAT経路の活性化を介し細胞増殖能を制御する2018

    • Author(s)
      近藤亮太、石野孔祐、金谷洋平、高田英志、彭為霞、工藤光洋、和田龍一、谷合信彦、内田英二、内藤善哉
    • Organizer
      第107回日本病理学会総会
    • Related Report
      2018 Research-status Report
  • [Presentation] Proteomic analysis reveals protein A as a potential prognostic biomarker for endometrioid carcinoma2018

    • Author(s)
      Wei-Xia Peng、Kousuke Ishino、Shoko Kure、Takenori Fujii、Kiyoshi Teduka、Mitsuhiro Kudo、Ryuichi Wada、Zenya Naito
    • Organizer
      United States and Canadian Academy of Pathology 2018 Annual Meeting
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 液状検体で利用可能な新規膵がん腫瘍マーカーのタンパク質網羅的探索2017

    • Author(s)
      石野孔祐、工藤光洋、彭為霞、呉壮香、河本陽子、手塚潔、藤井雄文、恩田宗彦、和田龍一、内藤善哉
    • Organizer
      第106回日本病理学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] 肝細胞癌においてPDIA3はJak/STAT経路の活性化を介し細胞増殖能を制御する2017

    • Author(s)
      近藤亮太、石野孔祐、金谷洋平、高田英志、彭為霞、工藤光洋、和田龍一、谷合信彦、内田英二、内藤善哉
    • Organizer
      第106回日本病理学会総会
    • Related Report
      2017 Research-status Report
  • [Presentation] 胃癌におけるDNA損傷応答関連分子の発現:網羅的蛋白発現解析と病理組織学的検討2017

    • Author(s)
      新井洋紀、和田龍一、石野孔祐、工藤光洋、内藤善哉
    • Organizer
      第63回日本病理学会秋期特別総会
    • Related Report
      2017 Research-status Report
  • [Remarks] リサーチマップ 石野孔祐

    • URL

      https://researchmap.jp/60584878

    • Related Report
      2019 Annual Research Report
  • [Remarks] researchmap-石野孔祐

    • URL

      https://researchmap.jp/60584878

    • Related Report
      2018 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2021-02-19  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi