| Project/Area Number |
17K15768
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Chiba University |
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Principal Investigator |
Ishige Takayuki 千葉大学, 医学部附属病院, 臨床検査技師 (30757315)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | ビタミンD代謝物 / 質量分析 / 遺伝子多型 / 融解曲線分析 / ビタミンD代謝 / 遺伝子多型解析 / Immunoextraction / 1α,25-dihydroxyvitamin D / LC/MS/MS / Immunoextraction; / GC genotype / melting analysis / ビタミンD代謝動態 |
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| Outline of Final Research Achievements |
Vitamin D has various roles related to bone metabolism and immune regulation. To elucidation of vitamin D metabolism, the accurate quantification method of its metabolites is required. In the circulating blood, vitamin D and its metabolites bind to vitamin D-binding protein (DBP), encoded by GC gene. In this study, we developed accurate quantification methods of vitamin D metabolites using LC/MS/MS. We also developed a high-throughput GC genotyping method using melting analysis with locked nucleic acid-incorporating dual hybridization probe for improving mismatch discrimination.
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| Academic Significance and Societal Importance of the Research Achievements |
健常者におけるビタミンD代謝物の血清濃度とGC遺伝子多型の詳細な関連が明らかになり、各種疾患とビタミンDの関連を理解するのに有用になることと思われる。LC/MS/MSを用いた高感度かつ高精度な分析法の構築により、ビタミンD代謝動態がより精確に把握できるようになる。臨床検査としても利用することが可能と思われ、LC/MS/MSを用いることで測定費用を低く抑えることができ、放射性廃棄物の問題も軽減できる。
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