Comprehensive assessment of serum vitamin D metabolism by LC/MS/MS
Project/Area Number |
17K15768
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Multi-year Fund |
Research Field |
Laboratory medicine
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Research Institution | Chiba University |
Principal Investigator |
Ishige Takayuki 千葉大学, 医学部附属病院, 臨床検査技師 (30757315)
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Project Period (FY) |
2017-04-01 – 2020-03-31
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Project Status |
Completed (Fiscal Year 2019)
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Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2019: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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Keywords | ビタミンD代謝物 / 質量分析 / 遺伝子多型 / 融解曲線分析 / ビタミンD代謝 / 遺伝子多型解析 / Immunoextraction / 1α,25-dihydroxyvitamin D / LC/MS/MS / Immunoextraction; / GC genotype / melting analysis / ビタミンD代謝動態 |
Outline of Final Research Achievements |
Vitamin D has various roles related to bone metabolism and immune regulation. To elucidation of vitamin D metabolism, the accurate quantification method of its metabolites is required. In the circulating blood, vitamin D and its metabolites bind to vitamin D-binding protein (DBP), encoded by GC gene. In this study, we developed accurate quantification methods of vitamin D metabolites using LC/MS/MS. We also developed a high-throughput GC genotyping method using melting analysis with locked nucleic acid-incorporating dual hybridization probe for improving mismatch discrimination.
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Academic Significance and Societal Importance of the Research Achievements |
健常者におけるビタミンD代謝物の血清濃度とGC遺伝子多型の詳細な関連が明らかになり、各種疾患とビタミンDの関連を理解するのに有用になることと思われる。LC/MS/MSを用いた高感度かつ高精度な分析法の構築により、ビタミンD代謝動態がより精確に把握できるようになる。臨床検査としても利用することが可能と思われ、LC/MS/MSを用いることで測定費用を低く抑えることができ、放射性廃棄物の問題も軽減できる。
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Report
(4 results)
Research Products
(26 results)
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[Journal Article] Anti-FIRΔexon2, a splicing variant form of PUF60, auto-antibody is detected in the sera of esophageal squamous cell carcinoma.2019
Author(s)
Kobayashi S, Hiwasa T, Ishige T, Rahmutulla B, Kano M, Hoshino T, Minamoto T, Shimada H, Nomura F, Matsubara H, Matsushita K.
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Journal Title
Cancer Sci.
Volume: 4
Issue: 6
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Assessment by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry of the Effects of Preanalytical Variables on Serum Peptidome Profiles Following Long-Term Sample Storage.2018
Author(s)
Tsuchida S, Satoh M, Umemura H, Sogawa K, Takiwaki M, Ishige T, Miyabayashi Y, Iwasawa Y, Kobayashi S, Beppu M, Nishimura M, Kodera Y, Matsushita K, Nomura F
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Journal Title
Proteomics. Clinical applications
Volume: 15
Issue: 3
Pages: 47-53
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Surveillance evaluation of the standardization of assay values for serum total 25-hydroxyvitamin D concentration in Japan.2018
Author(s)
Ihara H, Kiuchi S, Ishige T, Nishimura M, Matsushita K, Satoh M, Nomura F, Yamashita M, Kitajima I, Tsugawa N, Okano T, Hirota K, Miura M, Totani M, Hashizume N; Nutrition Division, Japan Society of Clinical Chemistry.
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Journal Title
Ann Clin Biochem.
Volume: Epub ahead of print
Issue: 6
Pages: 0-0
DOI
Related Report
Peer Reviewed / Open Access
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