| Project/Area Number |
17K15975
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Osaka Medical College |
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Principal Investigator |
Ogura Takeshi 大阪医科大学, 医学部, 講師 (10633331)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | メタボローム解析 / 胆道癌 / 胆汁 / バイオマーカー |
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| Outline of Final Research Achievements |
50 uL of plasma (or serum) were added to 450 uL 181;L of methanol containing internal standards (Solution ID: H3304-1002, Human Metabolome Technologies, Inc., Tsuruoka, Japan) at 0℃186;C in order to inactivate enzymes. The extract solution was thoroughly mixed with 500 uL 181;L of chloroform and 200 uL 181;L of Milli-Q water and centrifuged at 2,300 ×g and 4uL 186;C for 5 min. The 350 uL 81;L of upper aqueous layer was centrifugally filtered through a Millipore 5-kDa cutoff filter to remove proteins. The filtrate was centrifugally concentrated and re-suspended in 50 uL 181;L of Milli-Q water for CE-MS analysis.
Finally, A was significant factor associated with malignancy.
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| Academic Significance and Societal Importance of the Research Achievements |
物質Aが同定された。本結果は、胆道癌の診断の際、胆汁細胞診を行うが、感度が低いこと、偽陽性が多いことが問題であった。しかし、胆汁中の物質Aを測定することで癌の補助診断に有用である可能性が示唆された。さらに前向き試験として行い、感度、特異度を算出することで、より確立された手法になる可能性が示唆される。
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