Analysis of virulence factors of non-tuberculous mycobacteria focus on alveolar macrophages
| Project/Area Number |
17K16226
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Infectious disease medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
IDE Shotaro 長崎大学, 医歯薬学総合研究科(医学系), 客員研究員 (20773336)
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| Project Period (FY) |
2017-04-01 – 2021-03-31
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| Project Status |
Completed (Fiscal Year 2020)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 非結核性抗酸菌 / 抗酸菌 / マクロファージ / NF-κB / Mycobacterium / NTM / MAC / 気管支拡張症 / 感染免疫 / 感染症 / 細菌 / 免疫学 / 微生物 |
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| Outline of Final Research Achievements |
The aim of this research was to analyze virulence factors of non-tuberculous mycobacteria (NTM) focusing on immune responses to alveolar macrophages (AM) . We collected 64 strains of NTM from patients with pulmonary non-tuberculous mycobacteriosis (pNTM). The NF-κB eGFP reporter lentiviral-tranceduced murine AM were stimulated with these clinical NTM strains in vitro, then activity of NF-κB expressed by luciferase was detected and inflammatory cytokines in supernatants were measured by ELISA. Distinct AM NF-κB activities was observed depending on NTM strains, e.g. M. kansasii and M. abscessus rough morphotype induced high activity of NF-κB. TNF-α production from AM was dependent on NF-κB, although IL-1β was independent. AM NF-κB activity and bronchial dilatation in pNTM showed an inverse correlation. These results suggested that the AM NF-κB response may relates to bronchial inflammation distraction in pNTM.
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| Academic Significance and Societal Importance of the Research Achievements |
pNTMは近年本邦で増加しているが、難治性であり長期間の治療を必要とする疾患である。本研究では、肺の重要な免疫細胞であるマクロファージの転写因子NF-κB誘導能が、pNTMの病型を規定しているか明らかにすることを目的とした。NTMの菌種ごとにマクロファージの免疫反応の違いが見られ、それがpNTMの病型に関連している可能性が示唆された。原因微生物と宿主との免疫反応を明らかにすることで、pNTMの病態および増悪因子の理解につながることが期待される。
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Report
(5 results)
Research Products
(10 results)
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[Presentation] Mycobacterium intracellulareによる骨・軟部組織感染症を繰り返した抗IFN-γ抗体陽性自己免疫疾患の一例2020
Author(s)
北御門孝, 山本和子, 芦澤信之, 平山達朗, 田代将人, 田中健之, 高園貴弘, 今村圭文, 宮崎泰可, 泉川公一, 柳原克紀, 迎寛
Organizer
第90回 日本感染症学会西日本地方会学術集会
Related Report
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