| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
|
|---|
| Outline of Final Research Achievements |
We examined the role of MTNR1A, 1B and DUSP22 in brain development and the pathophysiological significance of these gene abnormalities. Information of tissue and subcellular distribution of Dusp22 is largely unknown while information on the quality of commercially available antibodies is very limited. Therefore, we here produced a rabbit polyclonal antibody specific for Dusp22 and performed expression analyses of the molecule. While western blotting analyses clarified that Dusp22 was dominantly expressed in heart and skeletal muscle. Dusp22 was diffusely distributed in the cytoplasm and partially colocalized with actin cytoskeleton. Silencing of DUSP22 prevented dendrite development in cortical pyramidal cells in vivo. Knockdown of MTNR1A and 1B did not affected cell morphology.
|
