| Project/Area Number |
17K16302
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Embryonic/Neonatal medicine
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| Research Institution | Nagasaki University |
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Principal Investigator |
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
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| Keywords | 胎児特異的 / 胎児機能 / microRNA / 間葉系幹細胞 / 羊水 / mRNA / 羊膜 / 胎児 / 分子マーカー |
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| Outline of Final Research Achievements |
We selected 15 microRNAs(miRNAs) which shows significantlly higher expression in maternal blood cells than foetal and neonatal blood cells. We separated mesenchymal stem cells(MSCs) from the chorionic plate(CP-MSCs), chorionic villi(CV-MSCs) and decidula basalis (DB-MSCs) of human term placental tissue. miR-518b and miR-517a (placental-specific miRNAs) were clearly expressed in CP-MSCs and CV-MSCs of foetal origin, but were barely expressed in DB-MSCs of maternal origin. Futhermore, expression levels of placenta-specific miRNAs in CV-MSCs remained stable across different pregnancy phases. miR-518b was transfected in CV-MSCs and microarray analysis was used to screen for miR-518b target genes. placenta-derived MSCs, especially CV-MSCs, are a potential tool for investigating the role of placental miRNAs in pregnancy-related disorders.
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| Academic Significance and Societal Importance of the Research Achievements |
産科医療における出生前の胎児機能評価には限界が知られており、新たな検査法の開発が望まれている。母体血漿中には胎児・胎盤由来のcell-free mRNA (cff-mRNA)が流入している。その流入源のひとつは胎盤であることが知られているが、胎児由来のものも存在していると考えられる。母体血漿中における胎盤特異的cff-mRNA流入量の変化と胎盤機能との関連が示唆されていることより、胎児特異的miRNAは胎児機能を推定する分子マーカーとして利用できる可能性がある。また、胎盤由来間葉系幹細胞は、妊娠関連疾患の分子メカニズムの解明や、細胞治療の有用なツールとなりうることが期待される。
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