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MicroRNA profiling in stress-resilient and stress-susceptible mice

Research Project

Project/Area Number 17K16380
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Psychiatric science
Research InstitutionYamaguchi University

Principal Investigator

HIGUCHI Fumihiro  山口大学, 医学部附属病院, 講師 (60711249)

Project Period (FY) 2017-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2017: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywordsストレス / うつ病 / マイクロRNA / 動物モデル / 遺伝子発現 / ストレスレジリエンス / うつ
Outline of Final Research Achievements

MicroRNAs (miRNAs) are small noncoding RNA molecules that regulate gene expression post-transcriptionally. In this study, we measured miRNAs levels in the medial prefrontal cortex of two strains of mice, each of which demonstrate different behavioral responses to stress. We found that stressed B6 mice show normal sociality, whereas DBA mice exposed to smSDS exhibit a significantly less social interaction than non-defeated DBA mice. Thus, we developed B6 and DBA mice as stress-resilient and stress-susceptible strains, respectively. We then performed small RNA-seq analysis to measure miRNA levels within the medial prefrontal cortex in stressed and non-stressed B6 and DBA mice. Small RNA-seq data revealed a unique change in miRNA expression between stress-resilient B6 and stress-susceptible DBA mice. This study suggests that miRNA expression, influenced by genetic and environmental factors, may contribute to behavioral responses to stress.

Academic Significance and Societal Importance of the Research Achievements

本研究によってストレス適応・不適応マウスの脳内マイクロRNA発現を網羅的に検証したことで、ストレス感受性・レジリエンスに関与する候補マイクロRNAを抽出することができた。これは、気分障害や不安障害などを含むストレス性精神疾患の発症機序解明につながる可能性がある。また、これら精神疾患の治療・再発予防に対する新たな治療法の開発が期待できる。

Report

(3 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • Research Products

    (2 results)

All 2018 2017

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Altered plasma protein glycosylation in a mouse model of depression and in patients with major depression2018

    • Author(s)
      Yamagata Hirotaka、Uchida Shusaku、Matsuo Koji、Harada Kenichiro、Kobayashi Ayumi、Nakashima Mami、Higuchi Fumihiro、Watanuki Toshio、Matsubara Toshio、Watanabe Yoshifumi
    • Journal Title

      Journal of Affective Disorders

      Volume: 233 Pages: 79-85

    • DOI

      10.1016/j.jad.2017.08.057

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Presentation] ストレス適応機構に対するヒストン脱アセチル化酵素の役割の解析2017

    • Author(s)
      樋口文宏
    • Organizer
      日本生物学的精神医学会
    • Related Report
      2017 Research-status Report

URL: 

Published: 2017-04-28   Modified: 2020-03-30  

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