Research Project
Grant-in-Aid for Young Scientists (B)
Tumors and primary cultured cells show phenotypic heterogeneity, and two-dimensional (2D) cultured cells are useful for various assays used to study these cells. In this study, we developed a primary cultured organoid in 2D culture (2D organoid; 2DO) that maintained the clinical phenotypic heterogeneity of tumor samples. The parental tumor differentiation was preserved in our system, with gene expression similar to that in the parental tumor. In vitro drug sensitivity analysis can be conducted using the 2DO to reflect individual clinical courses. The 2DO developed in this study is an in vitro personal cancer model. This approach may be useful for producing clinical chemotherapy agents as personalized medicines.
我々の樹立した初代培養細胞は基本的な培養方法を2次元培養としており(two-dimensional (2D) cultured organoid, 2DO)、非常に汎用性がある手法で、薬剤感受性試験を含む様々な研究手法において簡便に用いることができるマテリアルである。2DOを用いることで、より臨床の腫瘍に近い細胞挙動の解析が行え、多くのがん研究や個別化医療における有用なソースとなり得る。
All 2019 2018
All Journal Article (3 results) (of which Int'l Joint Research: 3 results, Peer Reviewed: 3 results, Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 3 results)
Biochemical and Biophysical Research Communications
Volume: in press Issue: 2 Pages: 332-339
10.1016/j.bbrc.2019.03.173
Stem Cells International
Volume: in press
Surg Today
Volume: in press Issue: 7 Pages: 709-715
10.1007/s00595-018-1644-9