Molecular mechanism of APOBEC-mediated HPV carcinogenesis and its application as prognosis factor
| Project/Area Number |
17K16839
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | National Institute of Infectious Diseases (2018)
Kanazawa University (2017) |
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Principal Investigator |
Wakae Kousho 国立感染症研究所, ウイルス第二部, 主任研究官 (70638303)
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| Project Period (FY) |
2017-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | APOBEC / HPV / 子宮頸癌 / 遺伝子変異 / hypermutation |
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| Outline of Final Research Achievements |
We clarified the mechanism how oncogenic host factors, APOBECs, are induced in HPV infected cells. We demonstrated that, differentiation of an HPV16(+) cervical keratinocyte induces APOBEC3A and 3B, as well as mutates host genome (Wakae, Sci. Rep. 2018). In addition, host gene mutations were detected from HPV-associated cancers, including cervical and oropharyngeal cancers, and were correlated with APOBEC3B expression. These results suggested that differentiation of HPV-infected host cells induces APOBECs and mutates their own genome.
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| Academic Significance and Societal Importance of the Research Achievements |
子宮頸がんや中咽頭癌の原因ウイルスであるヒトパピローマウイルス(HPV)が、どのような状況で発癌因子APOBECを発現し、遺伝情報を改変するのか、機序の一端を解明した。ウイルス学的・分子生物学的に重要な発見であると共に、HPV-APOBECによる発現機構を解明する事で、HPV関連癌の新しい予防・治療戦略の創出に貢献すると考えられる。
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Report
(3 results)
Research Products
(12 results)
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[Journal Article] Keratinocyte differentiation induces APOBEC3A, 3B, and mitochondrial DNA hypermutation2018
Author(s)
Wakae Kousho、Nishiyama Tomoaki、Kondo Satoru、Izuka Takashi、Que Lusheng、Chen Cong、Kase Kina、Kitamura Kouichi、Mohiuddin Md、Wang Zhe、Ahasan Md Monjurul、Nakamura Mitsuhiro、Fujiwara Hiroshi、Yoshizaki Tomokazu、Hosomochi Kazuyoshi、Tajima Atsushi、Nakahara Tomomi、Kiyono Tohru、Muramatsu Masamichi
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 9745-9745
DOI
NAID
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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