Non-invasive diagnostic methods using digital PCR for childhood cancers
| Project/Area Number |
17K17002
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pediatric surgery
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| Research Institution | Hiroshima University |
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Principal Investigator |
Kurihara Sho 広島大学, 医系科学研究科(医), 助教 (40724894)
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| Project Period (FY) |
2017-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2018: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2017: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | リキッドバイオプシー / デジタルPCR / 遊離DNA / マイクロRNA / 小児がん / 診断 / 治療効果判定 / 再発マーカー / 小児腫瘍学 / 遊離ＤＮＡ / 神経芽腫 / 肝芽腫 |
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| Outline of Final Research Achievements |
The MYCN amplification and ALK mutations in neuroblastoma, β-catenin gene aberrations in hepatoblastoma, and chimeric genes in alveolar rhabdomyosarcoma and Ewing family sarcomas were detectable by digital PCR methods using cell-free DNA in the serum samples of approximately 100 cases of childhood solid malignant tumors. In neuroblastoma, specific miRNAs correlated with malignant grade were also detectable. In particular, the sensitivity and specificity of MYCN amplification and β-catenin gene mutations using digital PCR were high, and these might be extremely useful not only for diagnosis but also for evaluation of therapeutic efficiency and monitoring of recurrence.
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| Academic Significance and Societal Importance of the Research Achievements |
小児腫瘍では、腫瘍特性の診断や悪性度判定は治療に不可欠であり、従来は、腫瘍生検にて腫瘍細胞を採取して検討されてきた。しかし、小児に対する腫瘍生検は全身麻酔が必要でさらに、部位により必ずしも容易でない。そのために、今回の研究によって血中や体液中遊離DNAを用いた非侵襲的検査によるリキッドバイオプシーにて診断や予後予測が可能となった重要性は大きい。こうしたリキッドバイオプシーは、今後臨床応用に向けて臨床試験などへの導入が望まれる。
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Report
(4 results)
Research Products
(22 results)
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[Journal Article] 「術後3年5ヵ月で再手術を要した巨大水腎症の1例 遅発性再発の要因についての一考察」2018
Author(s)
Tanaka M, Kohashi K, Kushitani K, Yoshida M, Kurihara S, Kawashima M, Ueda Y, Souzaki R, Kinoshita Y, Oda Y, Takeshima Y, Hiyama E, Taguchi T, Tanaka Y.
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Journal Title
日本小児泌尿器科学会雑誌
Volume: 27
Pages: 62-87
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[Presentation] Late toxicities, especially second malignancies, with GWAS analysis in hepatoblastoma cases treated in the Japanese Study Group for Pediatric Liver Tumor protocol-22019
Author(s)
Hiyama E, Kurihara S, Ueda Y, Hishiki T, Ida K, Iehara T, Oue T, Yano M, Watanabe K, Hoshino K, Ko K, Tanaka Y
Organizer
ASCO Annual Meeting.
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[Presentation] Survival and delayed effects of risk-stratified hepatoblastoma patients treated in the JPLT-2 trial2018
Author(s)
Hiyama E, Kurihara S, Ueda Y, Hishiki T, Ida K, Iehara T, Oue T, Yano M, Watanabe K, Hoshino K, Ko K, Tanaka Y
Organizer
2018 ASCO Annual Meeting
Related Report
Int'l Joint Research
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[Presentation] Decline in serum A-fetoprotein level before tumor resection as a prognostic factor for Hepatoblastoma2018
Author(s)
Hiyama E, Ueda Y, Kurihara S, Aoki Y, Iehara T, Ida K, Kazama T, Kihira K, Takama Y, taguchi T, Hishiki T, Fujimura J, Hoshino K, Honda S, Matsumoto K, Mori M, Yano M, Yokoi A, watanabe K, Tanaka Y
Organizer
50th Congress of the International Society of Paediatric Oncology (SIOP)
Related Report
Int'l Joint Research
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[Presentation] Surgical and Interventional Treatment for ruptured hepatic Tumors in Japanese Study Group Experience2017
Author(s)
15.Hiyama E, Hishiki T, Hoshino K, Yokoi A, Tamata T, Kazama T, Honda S, Taguchi T, Kurihara S, Kawashima M, Ueda Y
Organizer
The 49th Congress of the International Society of Paediatric Oncology (SIOP2017)
Related Report
Int'l Joint Research
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